ログイン
言語:

WEKO3

  • トップ
  • ランキング
To
lat lon distance
To

Field does not validate



インデックスリンク

インデックスツリー

メールアドレスを入力してください。

WEKO

One fine body…

WEKO

One fine body…

アイテム

{"_buckets": {"deposit": "58474733-8fb8-4277-befb-47eb09713ca0"}, "_deposit": {"id": "33927", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "33927"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00033927", "sets": ["455", "562"]}, "item_6_alternative_title_1": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "アルツハイマー病の進行に関連する血清マイクロRNAバイオマーカーmiR-501-3p"}]}, "item_6_date_granted_51": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "2019-09-12"}]}, "item_6_degree_grantor_49": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "新潟大学"}]}]}, "item_6_degree_name_48": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士(医学)"}]}, "item_6_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "MicroRNAs (miRNAs) are attractive molecules to utilize as one of the blood-based biomarkers for neurodegenerative disorders such as Alzheimer’s disease (AD) because miRNAs are relatively stable in biofluid, including serum or plasma. To determine blood miRNA biomarkers for AD with next-generation sequencing genome-wide, we first surveyed 45 serum samples. These came from 27 AD patients and 18 controls (discovery set) that underwent autopsy within two weeks after their serum sampling and were neuropathologically diagnosed. We found that three miRNAs, hsa-miR-501-3p, hsa-let-7f-5p, and hsa-miR-26b-5p, were significantly deregulated between the AD samples and the controls. The deregulation for hsa-miR-501-3p was further confirmed by quantitative reverse transcription polymerase chain reaction (PCR) in a validation set composed of 36 clinically diagnosed AD patients and 22 age-matched cognitively normal controls with a sensitivity and specificity of 53% and 100%, respectively (area under the curve = 0.82). Serum hsa-miR-501-3p levels were downregulated in AD patients, and its lower levels significantly correlated with lower Mini-Mental State Examination scores. Contrary to its serum levels, we found that hsa-miR-501-3p was remarkably upregulated in the same donors’ AD brains obtained at autopsy from the discovery set. The hsa-miR-501-3p overexpression in cultured cells, which mimicked the hsa-miR-501-3p upregulation in the AD brains, induced significant downregulation of 128 genes that overrepresented the Gene Ontology terms, DNA replication, and the mitotic cell cycle. Our results suggest that hsa-miR-501-3p is a novel serum biomarker that presumably corresponds to pathological events occurring in AD brains.", "subitem_description_type": "Abstract"}]}, "item_6_description_5": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "Acta Neuropathologica Communications. 2017, 5(1), 10.", "subitem_description_type": "Other"}]}, "item_6_description_53": {"attribute_name": "学位記番号", "attribute_value_mlt": [{"subitem_description": "新大博(医)乙第1810号", "subitem_description_type": "Other"}]}, "item_6_dissertation_number_52": {"attribute_name": "学位授与番号", "attribute_value_mlt": [{"subitem_dissertationnumber": "13101乙第2238号"}]}, "item_6_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "177896", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "原, 範和"}]}]}, "item_6_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "info:doi/10.1186/s40478-017-0414-z", "subitem_relation_type_select": "DOI"}}]}, "item_6_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "【○!C】 The Author(s). 2017"}]}, "item_6_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "ETD"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Hara, Norikazu"}], "nameIdentifiers": [{"nameIdentifier": "177895", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-07-08"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "r1nmo1810.pdf", "filesize": [{"value": "751.6 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 751600.0, "url": {"label": "本文", "url": "https://niigata-u.repo.nii.ac.jp/record/33927/files/r1nmo1810.pdf"}, "version_id": "06c54ce1-7fea-4fdd-90da-db5603f0bb7b"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-07-08"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "r1nmo1810_a.pdf", "filesize": [{"value": "372.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 372300.0, "url": {"label": "要旨", "url": "https://niigata-u.repo.nii.ac.jp/record/33927/files/r1nmo1810_a.pdf"}, "version_id": "45a5db46-511e-4930-b253-d1465feffb02"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Alzheimer’s disease", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Blood-based biomarker", "subitem_subject_scheme": "Other"}, {"subitem_subject": "microRNA", "subitem_subject_scheme": "Other"}, {"subitem_subject": "miR-501-3p", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Next-generation sequencing", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Autopsied brain", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Braak staging", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "thesis", "resourceuri": "http://purl.org/coar/resource_type/c_46ec"}]}, "item_title": "Serum microRNA miR-501-3p as a potential biomarker related to the progression of Alzheimer’s disease", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Serum microRNA miR-501-3p as a potential biomarker related to the progression of Alzheimer’s disease"}]}, "item_type_id": "6", "owner": "1", "path": ["455", "562"], "permalink_uri": "http://hdl.handle.net/10191/00051725", "pubdate": {"attribute_name": "公開日", "attribute_value": "2020-07-08"}, "publish_date": "2020-07-08", "publish_status": "0", "recid": "33927", "relation": {}, "relation_version_is_last": true, "title": ["Serum microRNA miR-501-3p as a potential biomarker related to the progression of Alzheimer’s disease"], "weko_shared_id": null}
  1. 0 資料タイプ別
  2. 02 学位論文
  1. 250 大学院医歯学総合研究科(医)
  2. 60 博士学位論文
  3. 10 博士学位論文

Serum microRNA miR-501-3p as a potential biomarker related to the progression of Alzheimer’s disease

http://hdl.handle.net/10191/00051725
http://hdl.handle.net/10191/00051725
59ef2039-7e37-4363-8239-1a6a9e8b7140
名前 / ファイル ライセンス アクション
r1nmo1810.pdf 本文 (751.6 kB)
r1nmo1810_a.pdf 要旨 (372.3 kB)
Item type 学位論文 / Thesis or Dissertation(1)
公開日 2020-07-08
タイトル
タイトル Serum microRNA miR-501-3p as a potential biomarker related to the progression of Alzheimer’s disease
言語
言語 eng
キーワード
主題Scheme Other
主題 Alzheimer’s disease
キーワード
主題Scheme Other
主題 Blood-based biomarker
キーワード
主題Scheme Other
主題 microRNA
キーワード
主題Scheme Other
主題 miR-501-3p
キーワード
主題Scheme Other
主題 Next-generation sequencing
キーワード
主題Scheme Other
主題 Autopsied brain
キーワード
主題Scheme Other
主題 Braak staging
資源タイプ
資源 http://purl.org/coar/resource_type/c_46ec
タイプ thesis
その他のタイトル
その他のタイトル アルツハイマー病の進行に関連する血清マイクロRNAバイオマーカーmiR-501-3p
著者 Hara, Norikazu

× Hara, Norikazu

WEKO 177895

Hara, Norikazu

Search repository
著者別名
識別子 177896
識別子Scheme WEKO
姓名 原, 範和
抄録
内容記述タイプ Abstract
内容記述 MicroRNAs (miRNAs) are attractive molecules to utilize as one of the blood-based biomarkers for neurodegenerative disorders such as Alzheimer’s disease (AD) because miRNAs are relatively stable in biofluid, including serum or plasma. To determine blood miRNA biomarkers for AD with next-generation sequencing genome-wide, we first surveyed 45 serum samples. These came from 27 AD patients and 18 controls (discovery set) that underwent autopsy within two weeks after their serum sampling and were neuropathologically diagnosed. We found that three miRNAs, hsa-miR-501-3p, hsa-let-7f-5p, and hsa-miR-26b-5p, were significantly deregulated between the AD samples and the controls. The deregulation for hsa-miR-501-3p was further confirmed by quantitative reverse transcription polymerase chain reaction (PCR) in a validation set composed of 36 clinically diagnosed AD patients and 22 age-matched cognitively normal controls with a sensitivity and specificity of 53% and 100%, respectively (area under the curve = 0.82). Serum hsa-miR-501-3p levels were downregulated in AD patients, and its lower levels significantly correlated with lower Mini-Mental State Examination scores. Contrary to its serum levels, we found that hsa-miR-501-3p was remarkably upregulated in the same donors’ AD brains obtained at autopsy from the discovery set. The hsa-miR-501-3p overexpression in cultured cells, which mimicked the hsa-miR-501-3p upregulation in the AD brains, induced significant downregulation of 128 genes that overrepresented the Gene Ontology terms, DNA replication, and the mitotic cell cycle. Our results suggest that hsa-miR-501-3p is a novel serum biomarker that presumably corresponds to pathological events occurring in AD brains.
内容記述
内容記述タイプ Other
内容記述 Acta Neuropathologica Communications. 2017, 5(1), 10.
DOI
識別子タイプ DOI
関連識別子 info:doi/10.1186/s40478-017-0414-z
権利
権利情報 【○!C】 The Author(s). 2017
著者版フラグ
値 ETD
学位名
学位名 博士(医学)
学位授与機関
学位授与機関名 新潟大学
学位授与年月日
学位授与年月日 2019-09-12
学位授与番号
学位授与番号 13101乙第2238号
学位記番号
内容記述タイプ Other
内容記述 新大博(医)乙第1810号
戻る
0
views
See details
Views

Versions

Ver.1 2021-03-01 08:08:43.422688
Show All versions

Share

Mendeley Twitter Facebook Print Addthis

Cite as

エクスポート

OAI-PMH
  • OAI-PMH JPCOAR
  • OAI-PMH DublinCore
  • OAI-PMH DDI
Other Formats
  • JSON
  • BIBTEX

Confirm


Powered by WEKO3


Powered by WEKO3