{"_buckets": {"deposit": "58474733-8fb8-4277-befb-47eb09713ca0"}, "_deposit": {"id": "33927", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "33927"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00033927", "sets": ["455", "562"]}, "item_6_alternative_title_1": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "アルツハイマー病の進行に関連する血清マイクロRNAバイオマーカーmiR-501-3p"}]}, "item_6_date_granted_51": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "2019-09-12"}]}, "item_6_degree_grantor_49": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "新潟大学"}]}]}, "item_6_degree_name_48": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士(医学)"}]}, "item_6_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "MicroRNAs (miRNAs) are attractive molecules to utilize as one of the blood-based biomarkers for neurodegenerative disorders such as Alzheimer’s disease (AD) because miRNAs are relatively stable in biofluid, including serum or plasma. To determine blood miRNA biomarkers for AD with next-generation sequencing genome-wide, we first surveyed 45 serum samples. These came from 27 AD patients and 18 controls (discovery set) that underwent autopsy within two weeks after their serum sampling and were neuropathologically diagnosed. We found that three miRNAs, hsa-miR-501-3p, hsa-let-7f-5p, and hsa-miR-26b-5p, were significantly deregulated between the AD samples and the controls. The deregulation for hsa-miR-501-3p was further confirmed by quantitative reverse transcription polymerase chain reaction (PCR) in a validation set composed of 36 clinically diagnosed AD patients and 22 age-matched cognitively normal controls with a sensitivity and specificity of 53% and 100%, respectively (area under the curve = 0.82). Serum hsa-miR-501-3p levels were downregulated in AD patients, and its lower levels significantly correlated with lower Mini-Mental State Examination scores. Contrary to its serum levels, we found that hsa-miR-501-3p was remarkably upregulated in the same donors’ AD brains obtained at autopsy from the discovery set. The hsa-miR-501-3p overexpression in cultured cells, which mimicked the hsa-miR-501-3p upregulation in the AD brains, induced significant downregulation of 128 genes that overrepresented the Gene Ontology terms, DNA replication, and the mitotic cell cycle. Our results suggest that hsa-miR-501-3p is a novel serum biomarker that presumably corresponds to pathological events occurring in AD brains.", "subitem_description_type": "Abstract"}]}, "item_6_description_5": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "Acta Neuropathologica Communications. 2017, 5(1), 10.", "subitem_description_type": "Other"}]}, "item_6_description_53": {"attribute_name": "学位記番号", "attribute_value_mlt": [{"subitem_description": "新大博(医)乙第1810号", "subitem_description_type": "Other"}]}, "item_6_dissertation_number_52": {"attribute_name": "学位授与番号", "attribute_value_mlt": [{"subitem_dissertationnumber": "13101乙第2238号"}]}, "item_6_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "177896", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "原, 範和"}]}]}, "item_6_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "info:doi/10.1186/s40478-017-0414-z", "subitem_relation_type_select": "DOI"}}]}, "item_6_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "【○!C】 The Author(s). 2017"}]}, "item_6_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "ETD"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Hara, Norikazu"}], "nameIdentifiers": [{"nameIdentifier": "177895", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-07-08"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "r1nmo1810.pdf", "filesize": [{"value": "751.6 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 751600.0, "url": {"label": "本文", "url": "https://niigata-u.repo.nii.ac.jp/record/33927/files/r1nmo1810.pdf"}, "version_id": "06c54ce1-7fea-4fdd-90da-db5603f0bb7b"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-07-08"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "r1nmo1810_a.pdf", "filesize": [{"value": "372.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 372300.0, "url": {"label": "要旨", "url": "https://niigata-u.repo.nii.ac.jp/record/33927/files/r1nmo1810_a.pdf"}, "version_id": "45a5db46-511e-4930-b253-d1465feffb02"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Alzheimer’s disease", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Blood-based biomarker", "subitem_subject_scheme": "Other"}, {"subitem_subject": "microRNA", "subitem_subject_scheme": "Other"}, {"subitem_subject": "miR-501-3p", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Next-generation sequencing", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Autopsied brain", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Braak staging", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "thesis", "resourceuri": "http://purl.org/coar/resource_type/c_46ec"}]}, "item_title": "Serum microRNA miR-501-3p as a potential biomarker related to the progression of Alzheimer’s disease", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Serum microRNA miR-501-3p as a potential biomarker related to the progression of Alzheimer’s disease"}]}, "item_type_id": "6", "owner": "1", "path": ["455", "562"], "permalink_uri": "http://hdl.handle.net/10191/00051725", "pubdate": {"attribute_name": "公開日", "attribute_value": "2020-07-08"}, "publish_date": "2020-07-08", "publish_status": "0", "recid": "33927", "relation": {}, "relation_version_is_last": true, "title": ["Serum microRNA miR-501-3p as a potential biomarker related to the progression of Alzheimer’s disease"], "weko_shared_id": null}