{"created":"2021-03-01T06:40:21.214900+00:00","id":33920,"links":{},"metadata":{"_buckets":{"deposit":"092db39d-e378-436a-b98e-649fb2151ef0"},"_deposit":{"id":"33920","owners":[],"pid":{"revision_id":0,"type":"depid","value":"33920"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00033920","sets":["453:455","471:561:562"]},"item_6_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Monitoring of Mitophagy using mito-Keima system"}]},"item_6_date_granted_51":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2019-09-20"}]},"item_6_degree_grantor_49":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"新潟大学"}]}]},"item_6_degree_name_48":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)"}]},"item_6_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"マイトファジーは、不要となったミトコンドリアをオートファジーを介して分解除去することで、ミトコンドリアの恒常性維持に寄与している。これまでの研究から、マイトファジーにはParkin依存的経路とParkin非依存的経路が知られているが、簡便で高感度の検出法はなかった。近年pH感受性蛍光タンパク質Keimaを用いたマイトファジー検出システム（mito-Keima）が報告された。本研究では、mito-KeimaシステムのParkin依存的経路・非依存的経路それぞれに対する感受性・特異性について、mito-Keima安定発現細胞株を用いて検討した。その結果、mito-Keima細胞株を用いた検出システムは、従来法に比べ高感度であり、特異性も高いことが明らかとなった。","subitem_description_type":"Abstract"}]},"item_6_description_53":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_description":"新大院博(医)甲第897号","subitem_description_type":"Other"}]},"item_6_dissertation_number_52":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"13101甲第4637号"}]},"item_6_relation_32":{"attribute_name":"異版あり","attribute_value_mlt":[{"subitem_relation_type":"hasVersion","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://hdl.handle.net/10191/51871","subitem_relation_type_select":"URI"}}]},"item_6_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"ETD"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Chernyshova, Kseniia"}],"nameIdentifiers":[{"nameIdentifier":"177884","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-07-08"}],"displaytype":"detail","filename":"r1nmk897.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文","url":"https://niigata-u.repo.nii.ac.jp/record/33920/files/r1nmk897.pdf"},"version_id":"4072d9f6-391a-4245-842b-36839669e661"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-07-08"}],"displaytype":"detail","filename":"r1nmk897_a.pdf","filesize":[{"value":"481.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要旨","url":"https://niigata-u.repo.nii.ac.jp/record/33920/files/r1nmk897_a.pdf"},"version_id":"83c23e5b-0f39-41c9-9125-923ac6f5ea0a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"マイトファジー","subitem_subject_scheme":"Other"},{"subitem_subject":"Parkin","subitem_subject_scheme":"Other"},{"subitem_subject":"mito-Keima","subitem_subject_scheme":"Other"},{"subitem_subject":"ミトコンドリア","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"mito-Keimaを用いたマイトファジーモニタリングシステムの検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"mito-Keimaを用いたマイトファジーモニタリングシステムの検討"}]},"item_type_id":"6","owner":"1","path":["455","562"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-07-08"},"publish_date":"2020-07-08","publish_status":"0","recid":"33920","relation_version_is_last":true,"title":["mito-Keimaを用いたマイトファジーモニタリングシステムの検討"],"weko_creator_id":"1","weko_shared_id":2},"updated":"2022-12-15T04:02:13.096822+00:00"}