{"created":"2021-03-01T06:39:55.430760+00:00","id":33486,"links":{},"metadata":{"_buckets":{"deposit":"5d387cff-c03d-4ad2-aee6-be19c5cb0505"},"_deposit":{"id":"33486","owners":[],"pid":{"revision_id":0,"type":"depid","value":"33486"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00033486","sets":["453:456","485:872:1583:1940"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Leaf color modification in Torenia concolor by ectopic expression of R2R3-MYB genes"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-02","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"42","bibliographicPageStart":"35","bibliographicVolumeNumber":"71","bibliographic_titles":[{"bibliographic_title":"新潟大学農学部研究報告"},{"bibliographic_title":"新潟大学農学部研究報告","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"黄葉・銅葉・赤葉などカラフルな色彩の葉をもつ植物はカラーリーフプランツと呼ばれ、近年その人気が高まっている。多くの場合、それらの葉の細胞ではアントシアニン類が多量に生合成・蓄積されている。アントシアニン類の生合成にはフラボノイド生合成酵素遺伝子の発現制御が関わっており、その転写因子としてR2R3-MYBが知られている。本研究では、宿根トレニア(Torenia concolor)におけるカラーリーフプランツの育成を目的として、ホトトギス(Tricyrtis sp.)およびシロイヌナズナ(Arabidopsis thaliana)由来R2R3-MYB遺伝子(TrMYB1およびPAP1)を異所発現する形質転換体の作出を試みた。導入したR2R3-MYB遺伝子の種類に関わらず、形質転換体は葉色の変化の程度により3タイプ(ほとんど変化なし、薄い赤紫色に変化、濃い赤紫色に変化)に分けることができた。アントシアニンの定量分析および外来R2R3-MYB遺伝子の発現解析を行ったところ、形質転換体における葉色の変化の程度と葉の総アントシアニン含量および外来遺伝子の発現レベルには相関がみられた。これらの結果から、宿根トレニアへのTrMYB1またはPAP1の導入および異所発現により、葉においてアントシアニン類の生合成・蓄積が誘導され、葉色が改変されることが示唆された。今後は、同様の手法を用いて、他の花き園芸植物におけるカラーリーフプランツの作出を検討する予定である。","subitem_description_type":"Abstract"},{"subitem_description":"Ornamental plants with red, purple and/or yellow leaves are generally called colored-leaf plants. Colored leaves are resulted from biosynthesis and accumulation of pigments in leaf cells. Among such pigments, anthocyanins are the most major ones, and they are synthesized through the flavonoid biosynthetic pathway. R2R3-MYB, a member of the MYB transcription factor family, is known to activate the flavonoid biosynthetic pathway. In the present study, we examined genetic transformation of Torenia concolor with R2R3-MYB genes from Tricyrtis sp. (TrMYB1) or Arabidopsis thaliana (PAP1) for producing novel colored-leaf plants. Totally ten and eight independent transgenic plants have so far been obtained by transformation with TrMYB1 and PAP1, respectively. Transgenic plants could be classified into three types according to the leaf color phenotype: green leaves as the wild-type plants (Type I), light red-purple leaves (Type II), and deep red-purple leaves (Type III). Three Type I, two Type II and five Type III transgenic plants were obtained for TrMYB1, whereas two Type I, three Type II and three Type III transgenic plants were obtained for PAP1. Total anthocyanin contents in leaves of Type III transgenic plants were significantly higher than the vector control. Real-time RT-PCR analysis showed that TrMYB1 and PAP1 expression levels generally correlated with the degree of leaf color alteration. These results indicate that ectopic expression of the heterogeneous R2R3-MYB genes in T. concolor may activate the flavonoid biosynthetic pathway in leaves leading to anthocyanin accumulation and leaf color alteration.","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"176471","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Fujimoto, Takuo"}]},{"nameIdentifiers":[{"nameIdentifier":"176472","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Takano, Miho"}]},{"nameIdentifiers":[{"nameIdentifier":"176473","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Otani, Masahiro"}]},{"nameIdentifiers":[{"nameIdentifier":"176474","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Nakano, Masaru"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟大学農学部"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00183393","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03858634","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"藤本, 卓生"}],"nameIdentifiers":[{"nameIdentifier":"176467","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高野, 美穂"}],"nameIdentifiers":[{"nameIdentifier":"176468","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大谷, 真広"}],"nameIdentifiers":[{"nameIdentifier":"176469","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"中野, 優"}],"nameIdentifiers":[{"nameIdentifier":"176470","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-09-19"}],"displaytype":"detail","filename":"71_35-42.pdf","filesize":[{"value":"789.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"71_35-42.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/33486/files/71_35-42.pdf"},"version_id":"966d2808-eef0-4e3b-850d-6daa8e1f4a3e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"R2R3-MYB 転写因子","subitem_subject_scheme":"Other"},{"subitem_subject":"アントシアニン","subitem_subject_scheme":"Other"},{"subitem_subject":"花き園芸植物","subitem_subject_scheme":"Other"},{"subitem_subject":"カラーリーフプランツ","subitem_subject_scheme":"Other"},{"subitem_subject":"フラボノイド生合成経路","subitem_subject_scheme":"Other"},{"subitem_subject":"anthocyanin","subitem_subject_scheme":"Other"},{"subitem_subject":"colored-leaf plant","subitem_subject_scheme":"Other"},{"subitem_subject":"flavonoid biosynthetic pathway","subitem_subject_scheme":"Other"},{"subitem_subject":"ornamental plant","subitem_subject_scheme":"Other"},{"subitem_subject":"R2R3-MYB transcription factor","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"宿根トレニアにおけるR2R3-MYB遺伝子を用いた形質転換による葉色の改変","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"宿根トレニアにおけるR2R3-MYB遺伝子を用いた形質転換による葉色の改変"},{"subitem_title":"宿根トレニアにおけるR2R3-MYB遺伝子を用いた形質転換による葉色の改変","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","1940"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-04-02"},"publish_date":"2019-04-02","publish_status":"0","recid":"33486","relation_version_is_last":true,"title":["宿根トレニアにおけるR2R3-MYB遺伝子を用いた形質転換による葉色の改変"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T04:03:39.459306+00:00"}