{"created":"2021-03-01T06:38:24.121921+00:00","id":32143,"links":{},"metadata":{"_buckets":{"deposit":"04fb8bb6-e39f-440f-9dcd-e4e0d630b6d6"},"_deposit":{"id":"32143","owners":[],"pid":{"revision_id":0,"type":"depid","value":"32143"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00032143","sets":["453:455","468:563:564"]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-03-25","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"72","bibliographicPageStart":"1","bibliographic_titles":[{}]}]},"item_6_date_granted_51":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2019-03-25"}]},"item_6_degree_grantor_49":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"新潟大学"}]}]},"item_6_degree_name_48":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（理学）"}]},"item_6_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"　After translation termination, the ribosome should be dissociated into large and small subunits for the next round of translation. In eukaryote and archaea, an ATP-binding cassette protein ABCE1 plays a crucial role in dissociation of the post-terminated ribosomal complex to ribosomal subunits in ATP dependent manner. However, the detailed mechanochemistry of its recruitment to the ribosome, ATPase activation, and subunit dissociation remain to be elucidated. Here I show that the ribosomal stalk protein, which is known to participate in the actions of translational GTPase factors, plays an important role in these events. Biochemical and crystal structural data indicate that the intrinsically disordered region at the C-terminus of the archaeal stalk protein aP1 binds to the nucleotide-binding domain 1 of aABCE1, and that this binding is crucial for ATPase activation of aABCE1 on the ribosome. The functional role of the ribosomal stalk•ABCE1 interaction in ATPase activation and the subunit dissociation is also investigated using mutagenesis in a yeast system. These results strongly suggested that the ribosomal stalk protein participates in efficient actions of eukaryotic and archaeal ribosome recycling. In conclusions, I first demonstrate that 1) the eukaryotic and archaeal ribosomal stalks participate in the ABCE1-dependent ribosomal subunit dissociation, and 2) the ribosomal stalk functions with not only with GTPase, but also with the ATPase translation factors.","subitem_description_type":"Abstract"},{"subitem_description":"　タンパク質を合成し終えたリボソームは、2つのリボソームサブユニットに解離し次の翻訳反応に再利用される。ATP加水分解酵素ABCE1は真核生物と古細菌に高度に保存されたリボソーム解離因子であり、ATP依存的にリボソームを解離する。しかしながら、ABCE1のリボソームへのリクルート機構、ATP加水分解の活性化機構、ATP加水分解とサブユニット解離の関連性は不明である。本研究では、GTP結合性翻訳因子のリボソームへのリクルートとそのGTP 加水分解の活性化を担うリボソームストークが、ATP加水分解酵素ABCE1の機能促進に重要であることが明らかとなった。生化学・構造生物学解析により、リボソームストークタンパク質aP1のC末端天然変性領域がaABCE1のヌクレオチド結合ドメイン1（NBD1）に結合することが示された。さらに、リボソームストーク・ABCE1間相互作用は、ABCE1のATP加水分解とそれに伴うリボソームサブユニットの解離反応に重要であることが生化学的解析および分子遺伝学的解析により明らかとなった。これらの結果から、（1）真核生物と古細菌のリボソームストークはABCE1依存のリボソーム解離反応に寄与すること、（2）リボソームストークはGTP結合性翻訳因子のみならずATP結合性翻訳因子の機能を促進することが明らかになった。","subitem_description_type":"Abstract"}]},"item_6_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"学位の種類: 博士（理学）. 報告番号: 甲第4608号. 学位記番号: 新大院博（理）甲第441号. 学位授与年月日: 平成31年3月25日","subitem_description_type":"Other"}]},"item_6_description_53":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_description":"新大院博（理）甲第441号","subitem_description_type":"Other"}]},"item_6_dissertation_number_52":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"13101甲第4608号"}]},"item_6_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟大学"}]},"item_6_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"ETD"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"今井, 大達"}],"nameIdentifiers":[{"nameIdentifier":"175283","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-09-10"}],"displaytype":"detail","filename":"h30fsk441.pdf","filesize":[{"value":"7.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文","url":"https://niigata-u.repo.nii.ac.jp/record/32143/files/h30fsk441.pdf"},"version_id":"137b095e-ef82-431a-a16e-0467b4ea409a"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-09-10"}],"displaytype":"detail","filename":"h30fsk441_a.pdf","filesize":[{"value":"169.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要旨","url":"https://niigata-u.repo.nii.ac.jp/record/32143/files/h30fsk441_a.pdf"},"version_id":"59de55e4-fc71-417c-86a6-0676201ac614"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"リボソームストークとATP加水分解酵素ABCE1の機能的相互作用","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"リボソームストークとATP加水分解酵素ABCE1の機能的相互作用"},{"subitem_title":"リボソームストークとATP加水分解酵素ABCE1の機能的相互作用","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["455","564"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-07-09"},"publish_date":"2019-07-09","publish_status":"0","recid":"32143","relation_version_is_last":true,"title":["リボソームストークとATP加水分解酵素ABCE1の機能的相互作用"],"weko_creator_id":"1","weko_shared_id":2},"updated":"2022-12-15T04:02:32.447132+00:00"}