{"created":"2021-03-01T06:38:21.421502+00:00","id":32103,"links":{},"metadata":{"_buckets":{"deposit":"9c4494a9-3507-46f9-8a56-1b15fe16f347"},"_deposit":{"id":"32103","owners":[],"pid":{"revision_id":0,"type":"depid","value":"32103"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00032103","sets":["432:433:434","453:455"]},"item_6_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"自己溶菌に伴い漏出する肺炎球菌のDNA結合タンパク質は，TLR4を介して炎症性サイトカインの産生を促進する"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-03-25","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"22","bibliographicPageStart":"14","bibliographic_titles":[{}]}]},"item_6_date_granted_51":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2019-03-25"}]},"item_6_degree_grantor_49":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"新潟大学"}]}]},"item_6_degree_name_48":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）"}]},"item_6_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Streptococcus pneumoniae is a leading cause of bacterial pneumonia. Our previous study suggested that S.pneumoniae autolysis-dependently releases intracellular pneumolysin, which subsequently leads to lung injury. In this study, we hypothesized that pneumococcal autolysis induces the leakage of additional intracellular molecules that could increase the pathogenicity of S. pneumoniae. Liquid chromatography tandem-mass spectrometry analysis identified that chaperone protein DnaK, elongation factor Tu (EF-Tu), and glyceraldehyde-3- phosphate dehydrogenase (GAPDH) were released with pneumococcal DNA by autolysis. We demonstrated that recombinant (r) DnaK, rEF-Tu, and rGAPDH induced significantly higher levels of interleukin-6 and tumor necrosis factor production in peritoneal macrophages and THP-1-derived macrophage-like cells via toll-like receptor 4. Furthermore, the DNA-binding activity of these proteins was confirmed by surface plasmon resonance assay. We demonstrated that pneumococcal DnaK, EF-Tu, and GAPDH induced the production of proinflammatory cytokines in macrophages, and might cause host tissue damage and affect the development of pneumococcal diseases.","subitem_description_type":"Abstract"}]},"item_6_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"学位の種類: 博士（歯学）. 報告番号: 甲第4568号. 学位記番号: 新大院博（歯）甲第417号. 学位授与年月日: 平成31年3月25日","subitem_description_type":"Other"},{"subitem_description":"Cellular Immunology. 2018, 325, 14-22.","subitem_description_type":"Other"}]},"item_6_description_53":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_description":"新大院博（歯）甲第417号","subitem_description_type":"Other"}]},"item_6_dissertation_number_52":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"13101甲第4568号"}]},"item_6_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"175224","nameIdentifierScheme":"WEKO"}],"names":[{"name":"永井, 康介"}]}]},"item_6_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟大学"}]},"item_6_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1016/j.cellimm.2018.01.006","subitem_relation_type_select":"DOI"}}]},"item_6_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"ETD"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nagai, Kosuke"}],"nameIdentifiers":[{"nameIdentifier":"175223","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-09-10"}],"displaytype":"detail","filename":"h30ndk417.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文","url":"https://niigata-u.repo.nii.ac.jp/record/32103/files/h30ndk417.pdf"},"version_id":"e2b5a44b-0f59-440a-975e-135b1c606051"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-09-10"}],"displaytype":"detail","filename":"h30ndk417_a.pdf","filesize":[{"value":"263.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要旨","url":"https://niigata-u.repo.nii.ac.jp/record/32103/files/h30ndk417_a.pdf"},"version_id":"e9db8b00-a4fd-4f0a-bea8-2816290957b3"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Streptococcus pneumoniae","subitem_subject_scheme":"Other"},{"subitem_subject":"Autolysis","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA-binding protein","subitem_subject_scheme":"Other"},{"subitem_subject":"DnaK","subitem_subject_scheme":"Other"},{"subitem_subject":"EF-Tu","subitem_subject_scheme":"Other"},{"subitem_subject":"GAPDH","subitem_subject_scheme":"Other"},{"subitem_subject":"TLR4","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"Pneumococcal DNA-binding proteins released through autolysis induce the production of proinflammatory cytokines via toll-like receptor 4","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Pneumococcal DNA-binding proteins released through autolysis induce the production of proinflammatory cytokines via toll-like receptor 4"},{"subitem_title":"Pneumococcal DNA-binding proteins released through autolysis induce the production of proinflammatory cytokines via toll-like receptor 4","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["455","434"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-07-09"},"publish_date":"2019-07-09","publish_status":"0","recid":"32103","relation_version_is_last":true,"title":["Pneumococcal DNA-binding proteins released through autolysis induce the production of proinflammatory cytokines via toll-like receptor 4"],"weko_creator_id":"1","weko_shared_id":2},"updated":"2022-12-15T04:01:12.279578+00:00"}