{"created":"2021-03-01T06:38:16.463926+00:00","id":32029,"links":{},"metadata":{"_buckets":{"deposit":"7033995c-6fca-4f26-b884-fa6567052f84"},"_deposit":{"id":"32029","owners":[],"pid":{"revision_id":0,"type":"depid","value":"32029"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00032029","sets":["453:455","471:561:562"]},"item_6_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"カルボキシターミナルの天然変性領域に依存した抗酸菌ヒストン様蛋白質による抗酸菌ゲノムの構造形成"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-09-20","bibliographicIssueDateType":"Issued"},"bibliographic_titles":[{}]}]},"item_6_date_granted_51":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2018-09-20"}]},"item_6_degree_grantor_49":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"新潟大学"}]}]},"item_6_degree_name_48":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）"}]},"item_6_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The architecture of the genome influences the functions of DNA from bacteria to eukaryotes. Intrinsically disordered regions (IDR) of eukaryotic histones have pivotal roles in various processes of gene expression. IDR is rare in bacteria, but interestingly, mycobacteria produce a unique histone-like protein, MDP1 that contains a long C-terminal IDR. Here we analyzed the role of IDR in MDP1 function. By employing Mycobacterium smegmatis that inducibly expresses MDP1 or its IDR-deficient mutant, we observed that MDP1 induces IDR-dependent DNA compaction. MDP1-IDR is also responsible for the induction of growth arrest and tolerance to isoniazid, a front line tuberculosis drug that kills growing but not growth-retardated mycobacteria. We demonstrated that MDP1-deficiency and conditional knock out of MDP1 cause spreading of the M. smegmatis genome in the stationary phase. This study thus demonstrates for the first time a C-terminal region-dependent organization of the genome architecture by MDP1, implying the significance of IDR in the function of bacterial histone-like protein.","subitem_description_type":"Abstract"}]},"item_6_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"学位の種類: 博士（医学）. 報告番号: 甲第4503号. 学位記番号: 新大院博（医）甲第840号. 学位授与年月日: 平成30年9月20日","subitem_description_type":"Other"},{"subitem_description":"Scientific Reports. 2018, 8, 8197.","subitem_description_type":"Other"}]},"item_6_description_53":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_description":"新大院博（医）甲第840号","subitem_description_type":"Other"}]},"item_6_dissertation_number_52":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"13101甲第4503号"}]},"item_6_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟大学"}]},"item_6_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1038/s41598-018-26463-9","subitem_relation_type_select":"DOI"}}]},"item_6_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"ETD"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Savitskaya, Anna"}],"nameIdentifiers":[{"nameIdentifier":"175091","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-09-10"}],"displaytype":"detail","filename":"h30nmk840.pdf","filesize":[{"value":"5.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文","url":"https://niigata-u.repo.nii.ac.jp/record/32029/files/h30nmk840.pdf"},"version_id":"feb3fb5a-5927-46b8-b7b2-6f73c20b5967"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-09-10"}],"displaytype":"detail","filename":"h30nmk840_a.pdf","filesize":[{"value":"521.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要旨","url":"https://niigata-u.repo.nii.ac.jp/record/32029/files/h30nmk840_a.pdf"},"version_id":"c7144b95-3fc0-4eeb-a9e8-b46f9783e195"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"C-terminal intrinsically disordered region-dependent organization of the mycobacterial genome by a histone-like protein","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"C-terminal intrinsically disordered region-dependent organization of the mycobacterial genome by a histone-like protein"},{"subitem_title":"C-terminal intrinsically disordered region-dependent organization of the mycobacterial genome by a histone-like protein","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["455","562"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-11-29"},"publish_date":"2018-11-29","publish_status":"0","recid":"32029","relation_version_is_last":true,"title":["C-terminal intrinsically disordered region-dependent organization of the mycobacterial genome by a histone-like protein"],"weko_creator_id":"1","weko_shared_id":2},"updated":"2022-12-15T04:02:22.071892+00:00"}