{"created":"2021-03-01T06:34:24.371589+00:00","id":28579,"links":{},"metadata":{"_buckets":{"deposit":"4d30cdde-70c0-4049-acb0-8e4fe5c80ae6"},"_deposit":{"id":"28579","owners":[],"pid":{"revision_id":0,"type":"depid","value":"28579"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00028579","sets":["453:456","506:1624:1625:1630"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Analysis of human T cell receptor Vβ repertoire of antigen specific cytotoxic T lymphocytes"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"74","bibliographicPageStart":"67","bibliographicVolumeNumber":"11","bibliographic_titles":[{"bibliographic_title":"新潟大学保健学雑誌"},{"bibliographic_title":"新潟大学保健学雑誌","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"抗原特異的細胞傷害性T細胞（CTL）のT細胞受容体（TCR）β鎖可変領域（Vβ）レパトワの同定は，より効果的なCTLを用いた免疫療法の確立に有効であると考える。そこで本研究では，混合リンパ球ペプチド培養（MLPC）法により誘導した抗原特異的CTLのTCR Vβレパトワ解析を行った。培養前の健常人末梢血単核球を用いたTCR Vβレパトワ解析では，CD8+T細胞は個々に異なる使用頻度で24種類のTCR Vβを使用していた。培養後，CD8+T細胞中のCTLの割合が高いウェルの多くでは，単一のTCR Vβの使用が確認された。いくつかのウェルにおいては，oligoclonalなTCR Vβの使用が見られた。ヒト白血球抗原（HLA）-A*24:02に拘束性を有する９残基のペプチドを用いた本検討において，誘導CTLは，moloclonalではなく，oligoclonalなTCR Vβを使用していることが示唆された。","subitem_description_type":"Abstract"},{"subitem_description":"Identification of T-cell receptor （TCR） gene element usage of antigen specific cytotoxic T lymphocytes （CTLs） could be beneficial in terms of establishing the efficient CTL-based immunotherapy. In this study, we tried to elucidate the usage of TCR β chain variable regions （Vβ） of WT1 or CMVpp65 specific CTLs by using TCR Vβ gene family-specific monoclonal antibodies and flow cytometric analysis. In pre-culture analysis, the usage of TCR Vβ was diverse in CD8+ T cells of all samples. Predominant samples from MLPC with high proportion of CD8+MHC tetramer+ cells within CD8+ T cells showed the usage of a single TCR Vβ gene. However, oligoclonal usage of the TCR Vβ genes was observed in cells from some wells of MLPC containing CD8+MHC tetramer+ cells. The type of TCR Vβ repertoire in MLPC cells from each well was not identical and showed oligoclonal feature from well to well. In the present study using WT1 or CMVpp65 peptides, it is suggested that TCR Vβ genes used by peptide-induced CTLs are oligoclonal features even with a certain HLA-A24:02 restricted 9 mer peptide.","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"164548","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Uchiyama, Takayoshi"}]},{"nameIdentifiers":[{"nameIdentifier":"164549","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Narita, Miwako"}]},{"nameIdentifiers":[{"nameIdentifier":"164550","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Kominami, Aki"}]},{"nameIdentifiers":[{"nameIdentifier":"164551","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Oiwa, Eri"}]},{"nameIdentifiers":[{"nameIdentifier":"164552","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Iwabuchi, Minami"}]},{"nameIdentifiers":[{"nameIdentifier":"164553","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Iwaya, Shunpei"}]},{"nameIdentifiers":[{"nameIdentifier":"164554","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Nishizawa, Yoshinori"}]},{"nameIdentifiers":[{"nameIdentifier":"164555","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Takahashi, Masuhiro"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟大学医学部保健学科"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12680484","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"21884617","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"内山, 孝由"}],"nameIdentifiers":[{"nameIdentifier":"164540","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"成田, 美和子"}],"nameIdentifiers":[{"nameIdentifier":"164541","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小南, 亜紀"}],"nameIdentifiers":[{"nameIdentifier":"164542","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大岩, 恵理"}],"nameIdentifiers":[{"nameIdentifier":"164543","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岩渕, 南"}],"nameIdentifiers":[{"nameIdentifier":"164544","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岩谷, 俊平"}],"nameIdentifiers":[{"nameIdentifier":"164545","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"西澤, 幹則"}],"nameIdentifiers":[{"nameIdentifier":"164546","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高橋, 益廣"}],"nameIdentifiers":[{"nameIdentifier":"164547","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-20"}],"displaytype":"detail","filename":"11(1)_67-74.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"11(1)_67-74.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/28579/files/11(1)_67-74.pdf"},"version_id":"e72a9641-8f21-4796-9a46-637ca231756d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"T細胞受容体（TCR）","subitem_subject_scheme":"Other"},{"subitem_subject":"β鎖可変領域（Vβ）レパトワ","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞傷害性T細胞（CTL）","subitem_subject_scheme":"Other"},{"subitem_subject":"CD8陽性（CD8+）T細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"T cell receptor（TCR）","subitem_subject_scheme":"Other"},{"subitem_subject":"TCR V βrepertoire","subitem_subject_scheme":"Other"},{"subitem_subject":"cytotoxic T lymphocyte （CTL）","subitem_subject_scheme":"Other"},{"subitem_subject":"CD8+ T cell","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"抗原特異的細胞傷害性T細胞のT細胞受容体β鎖可変領域レパトワの解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"抗原特異的細胞傷害性T細胞のT細胞受容体β鎖可変領域レパトワの解析"},{"subitem_title":"抗原特異的細胞傷害性T細胞のT細胞受容体β鎖可変領域レパトワの解析","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","1630"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-02-29"},"publish_date":"2016-02-29","publish_status":"0","recid":"28579","relation_version_is_last":true,"title":["抗原特異的細胞傷害性T細胞のT細胞受容体β鎖可変領域レパトワの解析"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:58:45.857976+00:00"}