@article{oai:niigata-u.repo.nii.ac.jp:00028576,
 author = {Matsuda, Yasunobu and Wakai, Toshifumi and Hirose, Yuki and Sakata, Jun and Kobayashi, Takashi and Osawa, Mami and Fujimaki, Shun and Kubota, Masayuki},
 issue = {1},
 journal = {新潟大学保健学雑誌, 新潟大学保健学雑誌},
 month = {Mar},
 note = {Background & Aims: The therapeutic potential of marrow stem cells for liver disease is currently unclear. The aim of this study was to investigate whether potentiating the chemotaxis ability of stromal cell-derived factor 1 could augment the engraftment of marrow stem cells and ameliorate tissue damage. Methods: Female mice reconstructed with β-galactosidase-positive male marrow cells were repeatedly injected with carbon tetrachloride to induce liver cirrhosis. The statuses of stromal cell-derived factor 1 and its inhibitor CD26 were examined by immunohistochemical staining, enzyme-linked immunosorbent assays and colorimetric assays. Recipients were treated with an inhibitory peptide for CD26（Diprotin A）together with granulocyte colony-stimulating factor, and the marrow-derived cells were tracked through the β-galactosidase activity or sex-determining region on the Y chromosome. Results: Stromal cell-derived factor 1 expression and CD26 activity were both significantly increased and marrow-derived hepatocytes were not observed in liver cirrhosis, suggesting that the potential of stem cell homing is impaired by liver damage. Granulocyte colony-stimulating factor only induced a few cell clusters comprising 2-6 marrow-derived hepatocytes at a late stage of liver cirrhosis. In contrast, when animals with liver cirrhosis were treated with Diprotin A after granulocyte colonystimulating factor-induced stem cell mobilization, marrow-derived hepatocytes increased to 2-4% of the total hepatocytes and both the serum albumin and hepatic hydroxyproline contents were ameliorated（p < .05）. Conclusions: Maintaining the chemotaxis ability of stromal cell-derived factor 1 may represent an effective strategy for validating stem cell therapy.},
 pages = {39--49},
 title = {Blockade of CD26 Augments the Therapeutic Potential of Marrow Stem Cells in the Liver},
 volume = {11},
 year = {2014}
}