{"created":"2021-03-01T06:34:20.584648+00:00","id":28522,"links":{},"metadata":{"_buckets":{"deposit":"95620afb-30ac-4748-8ada-35e9d2f1f223"},"_deposit":{"id":"28522","owners":[],"pid":{"revision_id":0,"type":"depid","value":"28522"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00028522","sets":["453:456","506:1624:1625:1626"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Antigen specific INF-γproduction of the amplified CTL using plasmacytoid dendritic cell line (PMDC11)"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"17","bibliographicPageStart":"9","bibliographicVolumeNumber":"15","bibliographic_titles":[{"bibliographic_title":"新潟大学保健学雑誌"},{"bibliographic_title":"新潟大学保健学雑誌","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"免疫チェックポイント阻害薬の臨床的効果により，抗原特異的細胞傷害性T細胞（CTL）が抗腫瘍作用において重要な役割を果たしていることが明らかとなった。腫瘍に対するCTLの臨床効果を予測する解析のためには，個別のヒトCTLの簡便なクローン性の増幅方法の開発が必要である。我々は，MLPC法と強力な抗原提示能を有する樹状細胞株PMDC11を用いてWT1特異的CTLのクローン性増幅を試みた。数個のCTLから４週間の培養によって増幅した細胞群にはWT1特異的IFN-γ産生が確認された。この抗原特異的CTLの簡便で効率的な増幅方法は，免疫チェックポイント阻害因子，T細胞受容体（TCR）や腫瘍特異的表面抗原の解析に応用可能と思われた。","subitem_description_type":"Abstract"},{"subitem_description":"It is becoming clear that antigen-specific cytotoxic T cells (CTLs) play an important role in anti-tumor immune system due to the recent confirmation of clinical effects of immune-checkpoint inhibitors. We tried to amplify efficiently WT1 specific oligoclonal CTLs from the peripheral blood of the case treated with WT1 peptide vaccine using dendritic cell line PMDC 11 which was established in our laboratory. Antigen - specific IFN - γ - producing ability was confirmed in the amplified WT1- specific CTLs. This result means that the antigen - specific CTLs amplified by PMDC 11 maintain cytotoxic ability against to tumor specific antigen. This method of amplifying antigen-specific CTLs using MLPC and PMDC 11 will lead to application of CTL to clinical application and TCR and surface antigen analysis.","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"164089","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Yamada, Syunya"}]},{"nameIdentifiers":[{"nameIdentifier":"164090","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Goto, Wakana"}]},{"nameIdentifiers":[{"nameIdentifier":"164091","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Ogawa, Sara"}]},{"nameIdentifiers":[{"nameIdentifier":"164092","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Masuko, Masayoshi"}]},{"nameIdentifiers":[{"nameIdentifier":"164093","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Kobayashi, Kaho"}]},{"nameIdentifiers":[{"nameIdentifier":"164094","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Shibasaki, Yasuhiko"}]},{"nameIdentifiers":[{"nameIdentifier":"164095","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Uchiyama, Takayoshi"}]},{"nameIdentifiers":[{"nameIdentifier":"164096","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Takizawa, Jun"}]},{"nameIdentifiers":[{"nameIdentifier":"164097","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Sone, Hirohito"}]},{"nameIdentifiers":[{"nameIdentifier":"164098","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Takahashi, Masuhiro"}]},{"nameIdentifiers":[{"nameIdentifier":"164099","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Narita, Miwako"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟大学医学部保健学科"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12680484","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"21884617","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"山田, 峻也"}],"nameIdentifiers":[{"nameIdentifier":"164078","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"後藤, 若奈"}],"nameIdentifiers":[{"nameIdentifier":"164079","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小川, 彩空"}],"nameIdentifiers":[{"nameIdentifier":"164080","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"増子, 正義"}],"nameIdentifiers":[{"nameIdentifier":"164081","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小林, 果歩"}],"nameIdentifiers":[{"nameIdentifier":"164082","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"柴崎, 康彦"}],"nameIdentifiers":[{"nameIdentifier":"164083","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"内山, 孝由"}],"nameIdentifiers":[{"nameIdentifier":"164084","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"瀧澤, 惇"}],"nameIdentifiers":[{"nameIdentifier":"164085","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"曽根, 博仁"}],"nameIdentifiers":[{"nameIdentifier":"164086","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高橋, 益廣"}],"nameIdentifiers":[{"nameIdentifier":"164087","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"成田, 美和子"}],"nameIdentifiers":[{"nameIdentifier":"164088","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-20"}],"displaytype":"detail","filename":"15(1)_9-17.pdf","filesize":[{"value":"2.9 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"15(1)_9-17.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/28522/files/15(1)_9-17.pdf"},"version_id":"9702dd0b-f070-4cf8-a8a4-ee3faa8ded71"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"WT1","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞傷害性T細胞（CTL）","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞内インターフェロンγ","subitem_subject_scheme":"Other"},{"subitem_subject":"PMDC11","subitem_subject_scheme":"Other"},{"subitem_subject":"antigen specific CTL","subitem_subject_scheme":"Other"},{"subitem_subject":"INF-γ","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"樹状細胞由来細胞株を用いて増幅したWT1特異的細胞傷害性T細胞（CTL）のINF-γ産生能の検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"樹状細胞由来細胞株を用いて増幅したWT1特異的細胞傷害性T細胞（CTL）のINF-γ産生能の検討"},{"subitem_title":"樹状細胞由来細胞株を用いて増幅したWT1特異的細胞傷害性T細胞（CTL）のINF-γ産生能の検討","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","1626"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-04-05"},"publish_date":"2018-04-05","publish_status":"0","recid":"28522","relation_version_is_last":true,"title":["樹状細胞由来細胞株を用いて増幅したWT1特異的細胞傷害性T細胞（CTL）のINF-γ産生能の検討"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:58:41.582148+00:00"}