@article{oai:niigata-u.repo.nii.ac.jp:00028521,
 author = {後藤, 若奈 and 山田, 峻也 and 橋本, 誠雄 and 増子, 正義 and 小林, 果歩 and 小川, 彩空 and 柴崎, 康彦 and 曽根, 博仁 and 高橋, 益廣 and 成田, 美和子},
 issue = {1},
 journal = {新潟大学保健学雑誌, 新潟大学保健学雑誌},
 month = {Mar},
 note = {本研究室では，樹立した白血病性形質細胞様樹状細胞株PMDC05へCD80遺伝子を導入することにより強力な抗原提示能をもつPMDC11を作成している。腫瘍細胞とその腫瘍に対する抗原特異的細胞傷害性T細胞 (CTL)との間の免疫チェックポイントの強度に関する検査法開発を目指した基礎的研究としてPMDC11のCpG-Bによる機能の変化の有無と腫瘍抗原特異的CTLの増幅効果を検討した。CpG-B刺激は共抑制因子PD-L1及びPD-L2の発現を増加させることなく共刺激因子CD40及びCD83をわずかに高めた。WT1ペプチドパルスに加えてCpG-B刺激したPMDC11とWT1/MHC-tetramer^+細胞との共培養を行ったところ， WT1ペプチド抗原特異的CTLの著しい増加が認められた。この培養法は，腫瘍抗原特異的CTLの機能解析において応用可能と考えられた。, The leukemic plasmacytoid dendritic cell line (PMDC05) transduced with CD80 gene, which was previously established in our laboratory, was named PMDC11. PMDC11 cells have potent antigen-presenting ability. In this study, for the purpose to develop methods to examine the appearance of the immune checkpoints between tumor cells and antigen-specific cytotoxic T cells (CTLs) and to analyze those CTL function, we tried the expansion of WT1 specific CTL using PMDC11 as APCs. PMDC11 stimulated with CpG-B for 24 hours slightly enhanced the co-stimulatory factors CD40 and CD83 without increasing the expression of co-inhibitory factor PD-L1 and PD-L2. PMDC11 cells, which were pulsed WT1 peptide and stimulated with CpG-B, were co-cultured with WT1 / MHC-tetramer+ cells for 24 hours. As a result, the amplification of WT1 peptide antigen specific CTL was observed remarkably. Therefore, this amplification method is useful for development of an examination method on the strength of the immunity checkpoint, and it can be applied in the functional analysis of the anti-tumor antigen-specific CTL.},
 pages = {1--8},
 title = {CpG-Bを用いた白血病性形質細胞様樹状細胞（PMDC11）における抗原特異的CTL誘導能の増強},
 volume = {15},
 year = {2018}
}