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PMDC11 cells have potent antigen-presenting ability. In this study, for the purpose to develop methods to examine the appearance of the immune checkpoints between tumor cells and antigen-specific cytotoxic T cells (CTLs) and to analyze those CTL function, we tried the expansion of WT1 specific CTL using PMDC11 as APCs. PMDC11 stimulated with CpG-B for 24 hours slightly enhanced the co-stimulatory factors CD40 and CD83 without increasing the expression of co-inhibitory factor PD-L1 and PD-L2. PMDC11 cells, which were pulsed WT1 peptide and stimulated with CpG-B, were co-cultured with WT1 / MHC-tetramer+ cells for 24 hours. As a result, the amplification of WT1 peptide antigen specific CTL was observed remarkably. Therefore, this amplification method is useful for development of an examination method on the strength of the immunity checkpoint, and it can be applied in the functional analysis of the anti-tumor antigen-specific CTL.", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "164068", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Goto, Wakana"}]}, {"nameIdentifiers": [{"nameIdentifier": "164069", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Yamada, Syunya"}]}, {"nameIdentifiers": [{"nameIdentifier": "164070", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Hashimoto, Shigeo"}]}, {"nameIdentifiers": [{"nameIdentifier": "164071", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Masuko, Masayoshi"}]}, {"nameIdentifiers": [{"nameIdentifier": "164072", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Kobayashi, Kaho"}]}, 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