@article{oai:niigata-u.repo.nii.ac.jp:00028518, author = {Ohyama, Takuji and Ooomote, Hitoshi}, journal = {新潟大学農学部研究報告, 新潟大学農学部研究報告}, month = {Mar}, note = {The fate of 15N in rice plants was investigated after 15N03- was applied in surface water as a top dressing fertilizer. From 15N analysis, it was suggested that NO3- was absorbed mainly by leaf sheaths for the first one or two days, then roots began to absorb NO3- thereafter. The absorbed 15N was more rapidly assimilated into the amino acids and protein of leaf blades and leaf sheaths than those of the roots. The labelling pattern of glutamine, glutamic acid, aspartic acid and alanine was rapid and very similar in each organ. Asparagine, a major temporary storage amino compound in rice plants gradually incorporated 15N, but the initial incorporation rate was relatively slow. Before NO3- addition, no NO3- was detected in all plant parts. Immediately, NO3- became detectable in the leaf sheaths within 4 hours after NO3- addition. However, NO3- could not be detected in the roots for one day after NO3- application. From the results obtained it was concluded that top-dressed NO3- could be absorbed by leaf sheaths of rice plants, and the N was rapidly transported to the leaf blades and assimilated there., 水稲を栽培した土耕ポットの田面水に15N標識硝酸カルシウムを追肥し,水稲植物体内での15Nの挙動を調べた。硝酸追肥直後,約1-2日目までは,15N3-は田面水に接している葉鞘部分から主に吸収された。その後,根からも硝酸吸収が行われた。葉鞘で吸収された硝酸は,そのまま葉身へ移行し,葉身で還元を受け急速に遊離のアミノ酸やタンパク質等に同化された。その一部は,篩管を通って葉鞘や根へ再移動している事が示唆された。どの器官でもグル夕ミソ,グル夕ミン酸,アスパラギン酸,アラニンの15Nの取り込みは急速で同じような継時変化を示した。稲の一時的な窒素の貯蔵形態と考えられているアスパラギンは徐々に15Nを取り込んだ。硝酸追肥直前に採取した植物では,どの部位でも硝酸は検出されなかった。硝酸を供与すると,葉鞘では4時間以内に硝酸の集積が検出されたが,根では一日後まで硝酸は検出できなかった。以上の結果から,水稲の葉鞘は田面水に追肥として施された硝酸を吸収することができ,その硝酸は主に葉身で還元同化されることが示された。}, pages = {101--110}, title = {Absorption and Assimilation of Top Dressed Nitrate in Rice Plants Cultivated in Paddy Soil}, volume = {43}, year = {1991} }