{"created":"2021-03-01T06:34:20.188995+00:00","id":28516,"links":{},"metadata":{"_buckets":{"deposit":"f4f5e3e4-f4dd-46e3-bb92-607c1afb54ef"},"_deposit":{"id":"28516","owners":[],"pid":{"revision_id":0,"type":"depid","value":"28516"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00028516","sets":["453:456","485:872:1583:1621"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Histochemical Observations of Glycogen Granules in Cultured Bovine Eggs and Embryos during the Early Development"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1996-03","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"42","bibliographicPageStart":"37","bibliographicVolumeNumber":"48","bibliographic_titles":[{"bibliographic_title":"新潟大学農学部研究報告"},{"bibliographic_title":"新潟大学農学部研究報告","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"卵核胞期のウシの卵胞卵子，卵胞卵子を体外で成熟させた未受精卵子とこれを体外で受精させたのち培養して発生させた精子侵入期の卵子から孵化胚盤胞期までの胚について，グリコゲソ含量とα-Glycerophosphate dehydrogenase（α-GDH）活性を組織化学的に調べた。グリコゲン穎粒は，1細胞期の卵子から拡張胚盤胞期までの胚では細胞質に常に多量含まれていたが，孵化胚盤胞では減少した。またα-GDH活性は，1細胞期の卵子から拡張胚盤胞期までの胚では中等度であったが，孵化胚盤胞では弱かった。","subitem_description_type":"Abstract"},{"subitem_description":"The amount of glycogen granules and the activity of α-glycerophosphate dehydrogenase (α-GDH) were histochemically examined in bovine eggs matured in vitro, and in embryos from the 2-cell to hatched blastocyst stages developed in vitro. For the demonstration of glycogen, eggs and embryos were treated with the periodic acid－Schiff with or without a preceding salivary test. The activity of α-GDH was demonstrated by the method of BARKA and ANDERSON. The amount of glycogen granules was abundant in 1-cell eggs at the germinal vesicle, unfertilized and sperm-penetrated stages, and in embryos from the 2-cell to expanded blastocyst stages, but less in hatched blastocysts. The activity ofα-GDH was moderate in eggs and embryos at the stages from 1-cell to expanded blastocyst, but weak in hatched blastocysts.","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"164027","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Niimura, Sueo"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟大学農学部"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00183393","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03858634","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"新村, 末雄"}],"nameIdentifiers":[{"nameIdentifier":"164026","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-20"}],"displaytype":"detail","filename":"KK_48_37-42.pdf","filesize":[{"value":"1.9 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KK_48_37-42.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/28516/files/KK_48_37-42.pdf"},"version_id":"473cb7c5-1079-4cf3-abe6-d1ca92ba3d08"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ウシ","subitem_subject_scheme":"Other"},{"subitem_subject":"培養卵子","subitem_subject_scheme":"Other"},{"subitem_subject":"培養初期胚","subitem_subject_scheme":"Other"},{"subitem_subject":"グリコゲン顆粒","subitem_subject_scheme":"Other"},{"subitem_subject":"組織化学","subitem_subject_scheme":"Other"},{"subitem_subject":"Cow","subitem_subject_scheme":"Other"},{"subitem_subject":"cultured egg","subitem_subject_scheme":"Other"},{"subitem_subject":"cultured embryo","subitem_subject_scheme":"Other"},{"subitem_subject":"glycogen granule","subitem_subject_scheme":"Other"},{"subitem_subject":"histochemistry","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"培養したウシの卵子と初期胚におけるグリコゲン穎粒の組織化学的観察","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"培養したウシの卵子と初期胚におけるグリコゲン穎粒の組織化学的観察"},{"subitem_title":"培養したウシの卵子と初期胚におけるグリコゲン穎粒の組織化学的観察","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","1621"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-01-31"},"publish_date":"2012-01-31","publish_status":"0","recid":"28516","relation_version_is_last":true,"title":["培養したウシの卵子と初期胚におけるグリコゲン穎粒の組織化学的観察"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:58:41.611817+00:00"}