{"created":"2021-03-01T06:34:20.121150+00:00","id":28515,"links":{},"metadata":{"_buckets":{"deposit":"b6bdce31-4687-4527-9ce2-7791b8c392a6"},"_deposit":{"id":"28515","owners":[],"pid":{"revision_id":0,"type":"depid","value":"28515"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00028515"},"item_7_alternative_title_1":{"attribute_name":"\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb","attribute_value_mlt":[{"subitem_alternative_title":"Callus Formation and Plant Regeneration in Immature Barley Embryos with Different Seed Age"}]},"item_7_biblio_info_6":{"attribute_name":"\u66f8\u8a8c\u60c5\u5831","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1997-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"110","bibliographicPageStart":"103","bibliographicVolumeNumber":"49","bibliographic_titles":[{"bibliographic_title":"\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a"},{"bibliographic_title":"\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"\u6284\u9332","attribute_value_mlt":[{"subitem_description":"\u30aa\u30aa\u30e0\u30ae\u672a\u719f\u80da\u304b\u3089\u306e\u30ab\u30eb\u30b9\u5f62\u6210\u80fd\u304a\u3088\u3073\u518d\u5206\u5316\u80fd\u306e\u54c1\u7a2e\u554f\u5dee\u7570\u3092,\u3068\u304f\u306b\u7a2e\u5b50\u4ee4\u306b\u7740\u76ee\u3057\u3066\u8abf\u67fb\u3057\u305f\u3002\u4f9b\u8a66\u54c1\u7a2e\u306f\u4e16\u754c\u5404\u5730\u7523\u306e\u683d\u57f9\u30aa\u30aa\u30e0\u30ae\u3067, 1990\u5e74\u306f11, 91\u5e74\u306f23\u54c1\u7a2e\u3067\u3042\u308b\u3002\u958b\u82b1\u5f8c, 12, 14, 16\u304a\u3088\u307318\u65e5\u76ee\u306e\u672a\u719f\u80da\u3092\u57f9\u990a\u3057\u305f\u3068\u3053\u308d, 16, 18\u65e5\u76ee\u306e\u80da\u304b\u3089\u306f,\u307b\u3068\u3093\u3069\u306e\u54c1\u7a2e\u3067\u30ab\u30eb\u30b9\u304c\u8a98\u5c0e\u3055\u308c\u305f\u3002\u4e00\u65b9, 12\u304a\u3088\u307314\u65e5\u76ee\u306e\u80da\u304b\u3089\u306e\u30ab\u30eb\u30b9\u5f62\u6210\u7387\u306b\u306f, 0 -100%\u306e\u8457\u3057\u3044\u54c1\u7a2e\u9593\u5dee\u7570\u304c\u8a8d\u3081\u3089\u308c\u305f\u3002\u54c1\u7a2e\u3068\u7a2e\u5b50\u4ee4\u3068\u306e\u4ea4\u4e92\u4f5c\u7528\u306f\u7d71\u8a08\u7684\u306b\u6709\u610f\u3067\u3042\u308a,\u3053\u306e\u6642\u671f\u306e\u672a\u719f\u80da\u3067\u306f\u30ab\u30eb\u30b9\u5f62\u6210\u80fd\u304c\u5927\u304d\u304f\u5909\u5316\u3057,\u305d\u306e\u7a0b\u5ea6\u304c\u54c1\u7a2e\u306b\u3088\u3063\u3066\u7570\u306a\u308b\u3053\u3068\u304c\u78ba\u8a8d\u3055\u308c\u305f\u3002\u518d\u5206\u5316\u80fd\u306b\u3064\u3044\u3066\u3082,\u30000 -17.2%\u306e\u54c1\u7a2e\u9593\u5dee\u7570\u304c\u898b\u3089\u308c, \u7a2e\u5b50\u4ee4\u306e\u82e5\u3044\u80da\u306b\u7531\u6765\u3057\u305f\u30ab\u30eb\u30b9\u3067\u518d\u5206\u5316\u80fd\u304c\u9ad8\u304b\u3063\u305f\u3002\u30ab\u30eb\u30b9\u5f62\u6210\u80fd, \u518d\u5206\u5316\u80fd\u3068\u3082\u306b\u54c1\u7a2e\u306e\u4ed6\u306b,\u7a2e\u5b50\u4ee4\u306e\u5f71\u97ff\u306e\u5927\u304d\u3044\u3053\u3068\u304c\u78ba\u8a8d\u3055\u308c,\u3053\u308c\u3089\u3068\u80da\u306e\u5185\u90e8\u7d44\u6210, \u3068\u304f\u306b\u690d\u7269\u30db\u30eb\u30e2\u30f3\u3068\u306e\u95a2\u9023\u304c\u8b70\u8ad6\u3055\u308c\u305f\u3002","subitem_description_type":"Abstract"},{"subitem_description":"The ability of callus formation and plant regeneration in immature barley embryos with different seed ages was investigated. In 1990 and 1991, 11 and 23 cultivars with different origins were used for the callus induction, respectively (Tab. 1). The immature embryos were sampled 12, 14, 16 and 18 days after anthesis, and cultured on MS-nledium supplemented with sucrose (20g/1), agar (8.5g/1) and 2, 4-D (2mg/1) and adjusted to pH 5.7. Twenty days after incubation under dark and 25\u00b11\u2103 condition, the rate of callus formation was recorded. Almost all of the embryos with seed age of 16 or 18 days induced calli, while those of 12- or 14- seed age showed quite different rates of callus formation among the cultivars; ranging from 0% to 82.2% in 12-day old embryos, alld from 53.6% to 100% in 14-day old embryos in 1990 (Tab. 2). The variation of calli induction was little in 12-day old embryos in 1991, but was marked in those with seed age of 14 days (3.3% to 92.6%) (Tab. 2). According to the analysis of variance, the differences in callus formation were significant among cultivars and seed ages (Tab. 3). It should be noted here that the interaction between cultivars and seed ages was significant both in 1990 and 1991. This implies that the rates of increase of callus induction differed among the genotypes in the period from 12 to 14 days after anthesis. The calli obtained were placed onto the illedium excluded 2, 4-D, and cultured under 14 hours day-length at 25\u00b11\u2103 for the plant regeneration. After incubation for 56 days, the rate of regeneration was studied. Calli derived from the younger embryos resulted in the higher rate of regeneration; 4.0, 2.4 and 1.6/6 were observed in embryos with 14, 16 and 18 days of seed age, respectively. The difference among the cultivars was remarkable in regeneration, railging from ll.9% to 0%. It was concluded that the ability of callus formation and regeneration was affected by not only the genotype but also the seed age of the embryo, and finally discussed on the relation of phytohormones to callus formation and regeneration.","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"\u8457\u8005\u5225\u540d","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"164024","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Fukuyama, Toshinori"}]},{"nameIdentifiers":[{"nameIdentifier":"164025","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Sato, Shingo"}]}]},"item_7_publisher_7":{"attribute_name":"\u51fa\u7248\u8005","attribute_value_mlt":[{"subitem_publisher":"\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8"}]},"item_7_select_19":{"attribute_name":"\u8457\u8005\u7248\u30d5\u30e9\u30b0","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00183393","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03858634","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"\u8457\u8005","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"\u798f\u5c71, \u5229\u7bc4"}],"nameIdentifiers":[{"nameIdentifier":"164022","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"\u4f50\u85e4, \u65b0\u543e"}],"nameIdentifiers":[{"nameIdentifier":"164023","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"\u30d5\u30a1\u30a4\u30eb\u60c5\u5831","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-20"}],"displaytype":"detail","filename":"49_2_103-110.pdf","filesize":[{"value":"614.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"49_2_103-110.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/28515/files/49_2_103-110.pdf"},"version_id":"9f813980-f9e4-4e75-879b-97e7a4040c49"}]},"item_keyword":{"attribute_name":"\u30ad\u30fc\u30ef\u30fc\u30c9","attribute_value_mlt":[{"subitem_subject":"\u30aa\u30aa\u30e0\u30ae","subitem_subject_scheme":"Other"},{"subitem_subject":"\u30ab\u30eb\u30b9\u5f62\u6210\u80fd","subitem_subject_scheme":"Other"},{"subitem_subject":"\u518d\u5206\u5316\u80fd","subitem_subject_scheme":"Other"},{"subitem_subject":"\u672a\u719f\u80da","subitem_subject_scheme":"Other"},{"subitem_subject":"\u7a2e\u5b50\u4ee4","subitem_subject_scheme":"Other"},{"subitem_subject":"barley","subitem_subject_scheme":"Other"},{"subitem_subject":"callus formation","subitem_subject_scheme":"Other"},{"subitem_subject":"regeneration","subitem_subject_scheme":"Other"},{"subitem_subject":"immature embryo","subitem_subject_scheme":"Other"},{"subitem_subject":"seed age","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"\u8a00\u8a9e","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"\u8cc7\u6e90\u30bf\u30a4\u30d7","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"\u7570\u306a\u308b\u7a2e\u5b50\u4ee4\u306e\u30aa\u30aa\u30e0\u30ae\u672a\u719f\u80da\u306b\u304a\u3051\u308b\u30ab\u30eb\u30b9\u5f62\u6210\u80fd\u304a\u3088\u3073\u518d\u5206\u5316\u80fd\u306e\u6bd4\u8f03","item_titles":{"attribute_name":"\u30bf\u30a4\u30c8\u30eb","attribute_value_mlt":[{"subitem_title":"\u7570\u306a\u308b\u7a2e\u5b50\u4ee4\u306e\u30aa\u30aa\u30e0\u30ae\u672a\u719f\u80da\u306b\u304a\u3051\u308b\u30ab\u30eb\u30b9\u5f62\u6210\u80fd\u304a\u3088\u3073\u518d\u5206\u5316\u80fd\u306e\u6bd4\u8f03"},{"subitem_title":"\u7570\u306a\u308b\u7a2e\u5b50\u4ee4\u306e\u30aa\u30aa\u30e0\u30ae\u672a\u719f\u80da\u306b\u304a\u3051\u308b\u30ab\u30eb\u30b9\u5f62\u6210\u80fd\u304a\u3088\u3073\u518d\u5206\u5316\u80fd\u306e\u6bd4\u8f03","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["453/456","485/872/1583/1620"],"pubdate":{"attribute_name":"\u516c\u958b\u65e5","attribute_value":"2018-09-11"},"publish_date":"2018-09-11","publish_status":"0","recid":"28515","relation_version_is_last":true,"title":["\u7570\u306a\u308b\u7a2e\u5b50\u4ee4\u306e\u30aa\u30aa\u30e0\u30ae\u672a\u719f\u80da\u306b\u304a\u3051\u308b\u30ab\u30eb\u30b9\u5f62\u6210\u80fd\u304a\u3088\u3073\u518d\u5206\u5316\u80fd\u306e\u6bd4\u8f03"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2021-03-01T10:58:23.191732+00:00"}