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  1. 0 資料タイプ別
  2. 03 紀要論文
  1. 070 農学部
  2. 20 紀要
  3. 01 新潟大学農学部研究報告
  4. 第54巻第2号

植物ホルモン分析のためのHPLCカラムの半値幅などの特性

http://hdl.handle.net/10191/24764
339dedb7-3e52-4b0d-924e-d5d68e9ced4b
名前 / ファイル ライセンス アクション
54_2_109-114.pdf 54_2_109-114.pdf (441.6 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2014-01-20
タイトル
タイトル 植物ホルモン分析のためのHPLCカラムの半値幅などの特性
タイトル
言語 en
タイトル 植物ホルモン分析のためのHPLCカラムの半値幅などの特性
言語
言語 jpn
キーワード
主題Scheme Other
主題 インドール酢酸
キーワード
主題Scheme Other
主題 アプシジン酸
キーワード
主題Scheme Other
主題 サイトカイニン
キーワード
主題Scheme Other
主題 ODS
キーワード
主題Scheme Other
主題 Phカラム
キーワード
主題Scheme Other
主題 indole-acetic acid
キーワード
主題Scheme Other
主題 abscisic acid
キーワード
主題Scheme Other
主題 cytokinin
キーワード
主題Scheme Other
主題 gibberellin
キーワード
主題Scheme Other
主題 PH column
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
その他のタイトル
その他のタイトル Various Properties such as Half-Band Width of HPLC Columns for Plant Hormone Analysis
著者 児島, 清秀

× 児島, 清秀

WEKO 68

児島, 清秀

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村田, 憲昭

× 村田, 憲昭

WEKO 163970

村田, 憲昭

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Wei, Min

× Wei, Min

WEKO 163971

Wei, Min

Search repository
千田, 佑子

× 千田, 佑子

WEKO 163972

千田, 佑子

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付, 立新

× 付, 立新

WEKO 163973

付, 立新

Search repository
著者別名
識別子
識別子 68
識別子Scheme WEKO
姓名
姓名 Kojima, Kiyohide
著者別名
識別子
識別子 163975
識別子Scheme WEKO
姓名
姓名 Murata, Yoshiaki
著者別名
識別子
識別子 163976
識別子Scheme WEKO
姓名
姓名 Chida, Yuuko
著者別名
識別子
識別子 163977
識別子Scheme WEKO
姓名
姓名 Fu, Lixin
抄録
内容記述タイプ Abstract
内容記述 植物ホルモンの分析のためにHPLCカラムの半値幅などの特牲を試験した。 ODSタイプのカラム(長さ25cm)で溶離液(0.5mL/分)のメタノール濃度の変更時(±20%)に、 15分間で保持時間は安定した。 6種類の植物ホルモンの標品に関して、溶離液のメタノール濃度と保持時間の関係をカラム毎に調ベた。ODSタイプのカラムでは溶出パターンの共通の傾向が確認できた。カラムを2本接続しても、カラム1本と同じ溶出パターンで、問題は生じなかった。この溶出パターンをメタノール濃度と保持時間ともに対数軸にプロットすると、曲線の力ープがゆるやかになり、両者の関係の傾向が確認できた。注入IAA量(溶質量)とビーク面積をプロットすると高い相関でほぽ比例していた。注入IAA量に関わらず、注入メタノール量が一定ならぱ半値幅は一定であった。半値幅と溶離液のメタノール濃度を対数軸にプロットすると、両者はほぼ直線関係になった。さらに、標品の半値幅と保持時間を対数軸にプロットすると、植物ホルモンの4つの標品は直線的な狭い帯状に存在した。
抄録
内容記述タイプ Abstract
内容記述 Various properties such as half-band width of HPLC columns for phytohormone analysis were examined. As methanol concentration (±20%) in eluent (0.5 mL/min) was changed using octadecylsilane (ODS) type column in 25 cm length, retention time became stable after 15 min. As to 6 standards of plant hormones, the relationship between methanol concentration in eluent and retention time was examined in each column. In ODS type column, the general tendency of elution pattern was confirmed. As two columns were connected, elution pattern was same as that of one column and no problem occurred. As in the elution pattern both MeOH concentration and retention time were plotted in log scale, extent of curve was gentle and the tendency of relationship between the two was confirmed. As injected indole-acetic acid (IAA) amount (solute amount) and peak area were plotted. correlation was high and there was proportional relationship. Regardless of injected IAA amount, as amount of injected MeOH was constant, value of half-band width was constant. As half-band width and MeOH concentration of eluent were plotted in log scale, they had almost straight relationship. Moreover, as half-band width and retention time were plotted in log scale, 4 standards of phytohormone exist in straight narrow belt.
書誌情報 新潟大学農学部研究報告
en : 新潟大学農学部研究報告

巻 54, 号 2, p. 109-114, 発行日 2002-03
出版者
出版者 新潟大学農学部
ISSN
収録物識別子タイプ ISSN
収録物識別子 03858634
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AN00183393
著者版フラグ
値 publisher
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