{"created":"2021-03-01T06:34:18.927212+00:00","id":28497,"links":{},"metadata":{"_buckets":{"deposit":"dddda604-2d83-42f2-ad04-1fdef6b7c10d"},"_deposit":{"id":"28497","owners":[],"pid":{"revision_id":0,"type":"depid","value":"28497"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00028497","sets":["453:456","485:872:1583:1611"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Retention property and Mass Spectrum of Labeled Standards of Cytokinins"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2003-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"90","bibliographicPageStart":"83","bibliographicVolumeNumber":"55","bibliographic_titles":[{"bibliographic_title":"新潟大学農学部研究報告"},{"bibliographic_title":"新潟大学農学部研究報告","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"サイトカイニン(CKs)の定量において、精度と感度の向上のために、安定同位体標識とコールド(非標識の天然型)標品のHPLCでの保持と MSでの質量スペクトルの特性を試験Lた。ODSとPhタイプのカラムの両方で、ゼアフチン(Z)とゼアチンリボシド(ZR)の両者とも、重水素ラベルの標品がコールドよりも早く溶出した。negative modeにおいて、ZRの m/z133/134と218の消長のパターンはZ と同様で、2H5-ZRでは分子イオンの m/z355巴断片イオンの133/134と223が見られた。 positive modeにおいて、 Z では、 20Vから60V で分子イオンの m/z220と断片の136が見られた。2H5-Z では、 Z の質量スペクトルの変化と同様に、分子イオンの m/z225と断片の137/136が見られた。 ZR では、 60V までは分子イオンのm/z352が見られ、 m/z136/137と220の消長のパターンはZ と同様であった。しかし、negative modeと同様に、 ZR の方が高いドリフト電圧が必要であった。2H5-ZRでは、分子イオンの m/z357と断片の m/z136/137と225が見られ、これらはZR および2H5-Z のビークのm/z と矛盾なく対応していた。全般的にpositive modeの intensityの値はnegative modeの10倍以上であるので、 Z と ZR の定量にはpositive mode が適する。","subitem_description_type":"Abstract"},{"subitem_description":"Properties of retention by HPLC and mass spectrums by mass spectrometer (MS) of stable isotope labeled and cold (non-labeled, natural type) standards were analyzed in cytokinins (CKs) quantification to improve accuracy and sensitivity. Both in zeatin (Z) and zeatin-9-riboside (ZR), deuterium labeled standards eluted earlier than cold standards in both columns of ODS and Ph types. In a negative mode, in ZR, the change patterns of m/z 133/134 and 218 were similar in Z, in 2H5-ZR, molecular ion, m/z 355 and fragment ions, 133/134 and 223 were observed. In a positive mode, in Z, molecular ion, m/z 220 and fragment ion, 136 were observed from 20 to 60V. In 2H5-Z, molecular ion, m/z 225 and fragment ion, 137/136 were observed as the change of mass spectrum of Z. In ZR, molecular ion, m/z 352 were observed till 60V, the change patterns of m/z 136/137 and 220 were similar in Z. However, ZR required higher drift voltage in the same as in negative mode. In 2H5-ZR, molecular ion, m/z 357 and fragment ions, 136/137 and 225 were observed, showing the consistent correspondence of peak m/z in ZR and 2H5-Z. The positive mode is suitable for the quantification of Z and ZR, because the intensity values of the positive mode were generally more than ten times than those of the negative mode.","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"68","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Kojima, Kiyohide"}]},{"nameIdentifiers":[{"nameIdentifier":"163925","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Yazawa, Sadayuki"}]},{"nameIdentifiers":[{"nameIdentifier":"163926","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Takahashi, Miyako"}]},{"nameIdentifiers":[{"nameIdentifier":"163927","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Shiota, Nozomi"}]},{"nameIdentifiers":[{"nameIdentifier":"163928","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Baba, Tadashi"}]},{"nameIdentifiers":[{"nameIdentifier":"163929","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Ikeda, Fukio"}]},{"nameIdentifiers":[{"nameIdentifier":"163930","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Nakano, Masaru"}]},{"nameIdentifiers":[{"nameIdentifier":"163931","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Han, Dong-sheng"}]},{"nameIdentifiers":[{"nameIdentifier":"163932","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Niimi, Yoshiji"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟大学農学部"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00183393","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03858634","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"児島, 清秀"}],"nameIdentifiers":[{"nameIdentifier":"68","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"谷澤, 貞幸"}],"nameIdentifiers":[{"nameIdentifier":"163916","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高橋, みや子"}],"nameIdentifiers":[{"nameIdentifier":"163917","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"塩田, 望"}],"nameIdentifiers":[{"nameIdentifier":"163918","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"馬場, 正"}],"nameIdentifiers":[{"nameIdentifier":"163919","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"池田, 富喜夫"}],"nameIdentifiers":[{"nameIdentifier":"163920","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"中野, 優"}],"nameIdentifiers":[{"nameIdentifier":"163921","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"韓, 東生"}],"nameIdentifiers":[{"nameIdentifier":"163922","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"新美, 芳二"}],"nameIdentifiers":[{"nameIdentifier":"163923","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-20"}],"displaytype":"detail","filename":"55_2_83-90.pdf","filesize":[{"value":"528.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"55_2_83-90.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/28497/files/55_2_83-90.pdf"},"version_id":"532fbb54-18cd-44a3-9668-189ff8334425"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"サイトカイニン","subitem_subject_scheme":"Other"},{"subitem_subject":"ゼアチン","subitem_subject_scheme":"Other"},{"subitem_subject":"ゼアチンリポシド","subitem_subject_scheme":"Other"},{"subitem_subject":"マススペクトル","subitem_subject_scheme":"Other"},{"subitem_subject":"cytokinins","subitem_subject_scheme":"Other"},{"subitem_subject":"zeatin","subitem_subject_scheme":"Other"},{"subitem_subject":"zeatin-9-riboside","subitem_subject_scheme":"Other"},{"subitem_subject":"mass spectrum","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"サイトカイニンの標識標品の保持特性と質量スペクトル","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"サイトカイニンの標識標品の保持特性と質量スペクトル"},{"subitem_title":"サイトカイニンの標識標品の保持特性と質量スペクトル","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","1611"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-01-20"},"publish_date":"2014-01-20","publish_status":"0","recid":"28497","relation_version_is_last":true,"title":["サイトカイニンの標識標品の保持特性と質量スペクトル"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:58:39.601355+00:00"}