{"created":"2021-03-01T06:34:18.467814+00:00","id":28490,"links":{},"metadata":{"_buckets":{"deposit":"48177839-608f-4fae-af34-3adeabfb90fa"},"_deposit":{"id":"28490","owners":[],"pid":{"revision_id":0,"type":"depid","value":"28490"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00028490","sets":["453:456","485:872:1583:1609"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Acquisition of Yeast Oxidosqualene-Lanosterol Cyclase Deficient Mutant"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2004-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"118","bibliographicPageStart":"113","bibliographicVolumeNumber":"56","bibliographic_titles":[{"bibliographic_title":"新潟大学農学部研究報告"},{"bibliographic_title":"新潟大学農学部研究報告","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"我々はオキシドスクアレン-ラノステロール環化酵素(OLC)の触媒機構を解明するため、酵母の発現糸を構築することを計画した。しかし、野生型の酵母は自身のOLCを持っていることから、そのまま発現宿主として用いることはできない。したがって、 OLC欠損型の酵母変異株を取得することを目的に実験を行った。酵母OLC欠損型二倍体から胞子形成を経て一倍体を分離した。得られた株(3-5-1)について、抗生物質に対する抵抗性の調査や脂質成分の分析そしてOLCの酵素活性の検定を行った。全ての結果は、 3-5-1株がOLC欠損型一倍体であることを示唆していた。今後、 3-5-1株は外来OLCの発現宿主として利用することができると考えられる。","subitem_description_type":"Abstract"},{"subitem_description":"In order to investigate the catalytic mechanism of oxidosqualene-lanosterol cyclase (OLC), we have planed to construct the expression systenl of OLC in yeast Saccharomyces cerevtsiae. However, wild-type yeast can not be used as the expression host of OLC from o山er organism, because yeasts naturally have its own OLC. Therefore, we made experiments to obtain the yeast OLC deficient mutant. The OLC deficient haploid was separated from the OLC deficient diploid after the sporulation. The obtained microorganism, named 3-5-1, was confirmed to be OLC deficient haploid by checking the resistance for antibiotics, analyzing the components of lipid and assaying the OLC activity. The strain, named 3-5-1, may be able to be used as the expression host of OLC from other organism in future.","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"163883","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Sato, Tsutomu"}]},{"nameIdentifiers":[{"nameIdentifier":"163884","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Hoshino, Tsutomu"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟大学農学部"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00183393","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03858634","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"佐藤, 努"}],"nameIdentifiers":[{"nameIdentifier":"163881","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"星野, 力"}],"nameIdentifiers":[{"nameIdentifier":"163882","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-20"}],"displaytype":"detail","filename":"56_2_113-118.pdf","filesize":[{"value":"404.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"56_2_113-118.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/28490/files/56_2_113-118.pdf"},"version_id":"00bdb36d-23e6-4530-9df6-115d90b3de54"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"オキシドスクアレン-ラノステロール環化酵素欠損型の酵母変異株の取得","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"オキシドスクアレン-ラノステロール環化酵素欠損型の酵母変異株の取得"},{"subitem_title":"オキシドスクアレン-ラノステロール環化酵素欠損型の酵母変異株の取得","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","1609"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-02-07"},"publish_date":"2014-02-07","publish_status":"0","recid":"28490","relation_version_is_last":true,"title":["オキシドスクアレン-ラノステロール環化酵素欠損型の酵母変異株の取得"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:58:40.383821+00:00"}