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First, a natural type of OLC was expressed in E.coli. However, it was insoluble in E.coli cell and had no enzymatic activity. Therefore, nine kind of altered OLCs were constructed on the basis of successful methods for other enzymes to increase the solubility or the suggestion from our experimental results for the squalene-hopene cyclase: a fused OLC with Trx or Dsb, a OLC deleted N-terminal domain and mutant OLCs having squalene-hopene cyclase types of QW motif. However, all types of OLC were insoluble and had no enzymatic activity. These results suggested that the expression host of OLC must be eucaryotic cells such as yeast and insect.","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"\u8457\u8005\u5225\u540d","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"163879","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Sato, Tsutomu"}]},{"nameIdentifiers":[{"nameIdentifier":"163880","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Hoshino, Tsutomu"}]}]},"item_7_publisher_7":{"attribute_name":"\u51fa\u7248\u8005","attribute_value_mlt":[{"subitem_publisher":"\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8"}]},"item_7_select_19":{"attribute_name":"\u8457\u8005\u7248\u30d5\u30e9\u30b0","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00183393","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03858634","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"\u8457\u8005","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"\u4f50\u85e4, \u52aa"}],"nameIdentifiers":[{"nameIdentifier":"163877","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"\u661f\u91ce, \u529b"}],"nameIdentifiers":[{"nameIdentifier":"163878","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"\u30d5\u30a1\u30a4\u30eb\u60c5\u5831","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-20"}],"displaytype":"detail","filename":"56_2_105-112.pdf","filesize":[{"value":"624.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"56_2_105-112.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/28489/files/56_2_105-112.pdf"},"version_id":"524b5e82-29da-43a0-956b-d298215b38ca"}]},"item_language":{"attribute_name":"\u8a00\u8a9e","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"\u8cc7\u6e90\u30bf\u30a4\u30d7","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Candida albicans\u7531\u6765\u306e\u30aa\u30ad\u30b7\u30c9\u30b9\u30af\u30a2\u30ec\u30f3-\u30e9\u30ce\u30b9\u30c6\u30ed\u30fc\u30eb\u74b0\u5316\u9175\u7d20\u306e\u5927\u8178\u83cc\u306b\u304a\u3051\u308b\u767a\u73fe\u306b\u3064\u3044\u3066","item_titles":{"attribute_name":"\u30bf\u30a4\u30c8\u30eb","attribute_value_mlt":[{"subitem_title":"Candida albicans\u7531\u6765\u306e\u30aa\u30ad\u30b7\u30c9\u30b9\u30af\u30a2\u30ec\u30f3-\u30e9\u30ce\u30b9\u30c6\u30ed\u30fc\u30eb\u74b0\u5316\u9175\u7d20\u306e\u5927\u8178\u83cc\u306b\u304a\u3051\u308b\u767a\u73fe\u306b\u3064\u3044\u3066"},{"subitem_title":"Candida albicans\u7531\u6765\u306e\u30aa\u30ad\u30b7\u30c9\u30b9\u30af\u30a2\u30ec\u30f3-\u30e9\u30ce\u30b9\u30c6\u30ed\u30fc\u30eb\u74b0\u5316\u9175\u7d20\u306e\u5927\u8178\u83cc\u306b\u304a\u3051\u308b\u767a\u73fe\u306b\u3064\u3044\u3066","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["453/456","485/872/1583/1609"],"pubdate":{"attribute_name":"\u516c\u958b\u65e5","attribute_value":"2014-02-07"},"publish_date":"2014-02-07","publish_status":"0","recid":"28489","relation_version_is_last":true,"title":["Candida albicans\u7531\u6765\u306e\u30aa\u30ad\u30b7\u30c9\u30b9\u30af\u30a2\u30ec\u30f3-\u30e9\u30ce\u30b9\u30c6\u30ed\u30fc\u30eb\u74b0\u5316\u9175\u7d20\u306e\u5927\u8178\u83cc\u306b\u304a\u3051\u308b\u767a\u73fe\u306b\u3064\u3044\u3066"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2021-03-01T10:59:05.428354+00:00"}