{"created":"2021-03-01T06:34:18.336759+00:00","id":28488,"links":{},"metadata":{"_buckets":{"deposit":"caf57c16-1d19-4b0f-a4c0-7855e326d4eb"},"_deposit":{"id":"28488","owners":[],"pid":{"revision_id":0,"type":"depid","value":"28488"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00028488","sets":["453:456","485:872:1583:1609"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"ブタ卵母細胞の核成熟に及ぼす各種ステロイドの影響"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2004-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"73","bibliographicPageStart":"67","bibliographicVolumeNumber":"56","bibliographic_titles":[{"bibliographic_title":"新潟大学農学部研究報告"},{"bibliographic_title":"新潟大学農学部研究報告","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The state of nuclear maturation was observed in porcine oocytes cultured with various steroids, in order to identify which steroids have a role of meiosis inducing substance (MIS) in porcine oocytes. In the oocytes cultured for 22 hrs, the percentage of oocytes with germinal vesicles (GVs) was significantly higher in those cultured with progesterone or 17α-hydroxyprogesterone (56.8 and 57.1 %) than in control oocytes (14.8 %), whereas the percentages of GV stage-oocytes cultured with cholesterol, pregnenolone, 17α-hydroxypregnenolone, 20α-hydroxyprogesterone or estradiol-17β (10.7 to 40.0 %) were similar to those of each control oocyte (14.8 to 67.9 %). At 44 hrs after maturation culture with various steroids, the percentages of oocytcs with the M Ⅱ stage nuclei were 42.9 to 91.7 % , showing no significant differences from those of each control oocyte (51.6 to 89.3 %). The developmental rates to 2-cell embryos of oocytes matured in the medium containing steroids also did not differ from those of each control oocyte, even in the use of either steroid examined. From the present findings, it was suggested that pregnenolone, progesterone, 17α-hydroxypregnenolone, 17α-hydroxyprogesterone, 20α-hydroxyprogesterone, estradiol-17β and cholesterol have no roles as MIS for porcine oocytes.","subitem_description_type":"Abstract"},{"subitem_description":"卵核抱期のブタ卵母細胞を各種ステロイドを含む培養液で培養し、核の成熟状態を観察した。22時間培養したブタ卵母細胞において、卵核胞期のものの割合は、プロゲステロンあるいは17α-ヒドロキシプロゲステロンと共に培養したものでは56.8および57.1%であり、対照の卵母細胞の14.8%に比べて有意に高かったが、コレステロール、プレグネノロン、 17α-ヒドロキシプレグネノロン、20α-ヒドロキシプロゲステロンあるいはエストラジオール-17βを含む培養液で培養したものでは10.7ないし40.0%であり、いずれもそれぞれの対照の卵母細胞の割合(14.8ないし67.9%)と相違なかった。また、各種ステロイドを含む培養液で44時間培養した卵母細胞において、第二成熟分裂中期に達したものの割合は、42.9ないし91.7%であり、いずれもそれぞれの対照の卵母細胞の割合(51.6ないし89.3%)と相違なかった。さらに、各種ステロイドを含む培養液で44時間培養した卵母細胞を媒精して48時間培養したところ、63.6ないし79.1%が2細胞胚に発生し、 2細胞胚への発生率は、いずれもそれぞれの対照の卵母細胞の発生率(72.5ないし86.2%)と相違なかった。以上の結果から、コレステロール、プレグネノロン、 17α-ヒドロキシプレグネノロン、プロゲステロン、 17α-ヒドロキシプロゲステロン、 20α-ヒドロキシプロゲステロンおよびエストラジオール-17βは、いずれもブタ卵母細胞の成熟分裂を誘起・促進する作用を持っていないことが推察された。","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"163875","nameIdentifierScheme":"WEKO"}],"names":[{"name":"高野, 裕子"}]},{"nameIdentifiers":[{"nameIdentifier":"163876","nameIdentifierScheme":"WEKO"}],"names":[{"name":"新村, 末雄"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟大学農学部"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00183393","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03858634","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Takano, Hiroko"}],"nameIdentifiers":[{"nameIdentifier":"163873","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Niimura, Sueo"}],"nameIdentifiers":[{"nameIdentifier":"163874","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-20"}],"displaytype":"detail","filename":"56_2_67-73.pdf","filesize":[{"value":"533.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"56_2_67-73.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/28488/files/56_2_67-73.pdf"},"version_id":"4f78c2cb-a07f-40cb-bcfc-a31fa8d2814a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"porcine oocyte","subitem_subject_scheme":"Other"},{"subitem_subject":"nuclear maturation","subitem_subject_scheme":"Other"},{"subitem_subject":"meiosis inducing substance","subitem_subject_scheme":"Other"},{"subitem_subject":"steroid","subitem_subject_scheme":"Other"},{"subitem_subject":"ブタ卵母細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"核成熟","subitem_subject_scheme":"Other"},{"subitem_subject":"成熟分裂誘起物質","subitem_subject_scheme":"Other"},{"subitem_subject":"ステロイド","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Effects of Steroids on Nuclear Maturation of Porcine Oocytes","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effects of Steroids on Nuclear Maturation of Porcine Oocytes"},{"subitem_title":"Effects of Steroids on Nuclear Maturation of Porcine Oocytes","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","1609"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-02-12"},"publish_date":"2014-02-12","publish_status":"0","recid":"28488","relation_version_is_last":true,"title":["Effects of Steroids on Nuclear Maturation of Porcine Oocytes"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:58:40.592466+00:00"}