{"_buckets": {"deposit": "559f6fec-e2ec-4ffb-9143-17e9c653fdf1"}, "_deposit": {"id": "28484", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28484"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028484"}, "item_7_alternative_title_1": {"attribute_name": "\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "Effect of Medium Composition on Soybean Seed Storage Protein, \u03b2Subunit of \u03b2 Conglycinin, mRNA Accumulation in In Vitro Immature Cotyledon Culture System"}]}, "item_7_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2004-08", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "45", "bibliographicPageStart": "41", "bibliographicVolumeNumber": "57", "bibliographic_titles": [{"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a"}, {"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "\u30c0\u30a4\u30ba\u7a2e\u5b50\u8caf\u8535\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u4e00\u3064\u3067\u3042\u308b\u03b2\u30b3\u30f3\u30b0\u30ea\u30b7\u30cb\u30f3\u306e\u03b2\u30b5\u30d6\u30e6\u30cb\u30c3\u30c8\u306e\u96c6\u7a4d\u8abf\u7bc0\u7269\u8cea\u3092\u691c\u7d22\u3059\u308b\u305f\u3081\u306b\u3001\u672a\u719f\u5b50\u8449\u306e\u8a66\u9a13\u7ba1\u57f9\u990a\u7cfb\u3092\u7528\u3044\u3066\u3044\u304f\u3064\u304b\u306e\u30a2\u30df\u30ce\u9178\u3001\u690d\u7269\u30db\u30eb\u30e2\u30f3\u53ca\u3073\u6709\u6a5f\u9178\u6dfb\u52a0\u306b\u5bfe\u3059\u308b\u5fdc\u7b54\u6027\u3092\u8abf\u67fb\u3057\u305f\u3002\u30b0\u30eb\u30bf\u30df\u30f3\u9178\u3001\u30a2\u30e9\u30cb\u30f3\u3092\u6dfb\u52a0\u3059\u308b\u3068\u3001\u672a\u719f\u5b50\u8449\u5185\u306e\u904a\u96e2\u30a2\u30df\u30ce\u9178\u304c\u4e0a\u6607\u3057\u3001\u3044\u304f\u3064\u304b\u306e\u30a2\u30df\u30ce\u9178\u304c\u5897\u52a0\u3057\u305f\u3053\u3068\u304b\u3089\u3001\u3053\u308c\u3089\u306e\u30a2\u30df\u30ce\u9178\u306f\u7a2e\u5b50\u306b\u53d6\u308a\u8fbc\u307e\u308c\u3001\u4ee3\u8b1d\u3055\u308c\u305f\u3053\u3068\u304c\u793a\u5506\u3055\u308c\u305f\u3002\u3057\u304b\u3057\u3001\u03b2\u30b5\u30d6\u30e6\u30cb\u30c3\u30c8mRNA\u306f\u30b0\u30eb\u30bf\u30df\u30f3\u6dfb\u52a0\u57f9\u5730\u4ee5\u5916\u3067\u306f\u691c\u51fa\u3067\u304d\u306a\u304b\u3063\u305f\u3002\u30a2\u30b9\u30d1\u30e9\u30ae\u30f3\u9178\u6dfb\u52a0\u3067\u306f\u672a\u719f\u5b50\u8449\u5185\u306e\u904a\u96e2\u30a2\u30df\u30ce\u9178\u6fc3\u5ea6\u306e\u4e0a\u6607\u53ca\u3073\u03b2\u30b5\u30d6\u30e6\u30cb\u30c3\u30c8mRNA\u306e\u96c6\u7a4d\u306f\u8a8d\u3081\u3089\u308c\u306a\u304b\u3063\u305f\u3002\u690d\u7269\u30db\u30eb\u30e2\u30f3\u306e\u4e00\u3064\u30b5\u30a4\u30c8\u30ab\u30a4\u30cb\u30f3(t-zeatin)\u306e\u6dfb\u52a0\u306b\u3088\u3063\u3066\u3082\u03b2\u30b5\u30d6\u30e6\u30cb\u30c3\u30c8\u306e\u96c6\u7a4d\u306f\u4fc3\u9032\u3055\u308c\u306a\u304b\u3063\u305f\u3002TCA\u56de\u8def\u306e\u03b1-\u30b1\u30c8\u30b0\u30eb\u30bf\u30eb\u9178\u3001\u30aa\u30ad\u30b5\u30ed\u9162\u9178\u3001\u30af\u30a8\u30f3\u9178\u306e\u6dfb\u52a0\u306b\u3088\u3063\u3066\u3082\u03b2\u30b5\u30d6\u30e6\u30cb\u30c3\u30c8mRNA\u306e\u96c6\u7a4d\u306f\u4fc3\u9032\u3055\u308c\u306a\u304b\u3063\u305f\u3002\u307e\u305f\u57f9\u990a\u5f8c\u306e\u672a\u719f\u5b50\u8449\u5185\u306b\u304a\u3051\u308b\u3053\u308c\u3089\u306e\u6709\u6a5f\u9178\u6fc3\u5ea6\u306b\u306f\u30b0\u30eb\u30bf\u30df\u30f3\u306e\u307f\u6dfb\u52a0\u306e\u5bfe\u7167\u533a\u3068\u6bd4\u8f03\u3057\u3066\u6709\u610f\u306a\u4e0a\u6607\u306f\u8a8d\u3081\u3089\u308c\u306a\u304b\u3063\u305f\u3002\u4ee5\u4e0a\u306e\u5b9f\u9a13\u7d50\u679c\u304b\u3089\u3001\u30c0\u30a4\u30ba\u672a\u719f\u5b50\u8449\u306b\u304a\u3044\u3066\u5c11\u306a\u304f\u3068\u3082\u4eca\u56de\u8a66\u9a13\u306b\u7528\u3044\u305f\u7269\u8cea\u306e\u5b50\u8449\u5916\u6fc3\u5ea6\u306e\u4e0a\u6607\u306b\u3088\u308a\u03b2\u30b5\u30d6\u30e6\u30cb\u30c3\u30c8mRNA\u306e\u96c6\u7a4d\u306f\u4fc3\u9032\u3055\u308c\u306a\u3044\u3053\u3068\u304c\u78ba\u8a8d\u3055\u308c\u305f\u3002It has been reported that the accumulation of soybean (Glycine max L.) storage protein, \u03b2 subunit of \u03b2 conglycinin, was suppressed by nitrogen (N)-deficiency. In this report we tried to evaluate what compound(s) may have a key role in regulating the accumulation of \u03b2 subunit mRNA in vitro cotyledon culture system. The cotyledons from non-nodulating soybean plants cultivated under N-deficient condition (0.5 mM NO 3 -) were transferred to in vitro culture system. The application of glutamine (Gln) enhanced \u03b2 subunit mRNA accumulation within twelve hours. The application of glutamic acid, alanine, or aspertic acid could not induced \u03b2 subunit mRNA accumulation. Application of cytokinin (t-zeatin) oxaloasetate, 2 -oxoglutarate and citrate could not enhance the accumulation of \u03b2 subunit mRNA under N free culture medium. These results suggested that at the least application to theses amino acids, orgamic acids and cytokinin could not be a direct signal to enhance \u03b2 subunit mRNA accumulation in invitro cotyledon culture system.", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "163857", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Ohtake, Norikuni"}]}, {"nameIdentifiers": [{"nameIdentifier": "163858", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Ohkano, Sayuri"}]}, {"nameIdentifiers": [{"nameIdentifier": "163859", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Sueyoshi, Kuni"}]}, {"nameIdentifiers": [{"nameIdentifier": "52", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Ohyama, Takuji"}]}]}, "item_7_publisher_7": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": 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