{"created":"2021-03-01T06:34:14.004305+00:00","id":28423,"links":{},"metadata":{"_buckets":{"deposit":"24b3a11c-40fc-4379-b860-a223b6397cc4"},"_deposit":{"id":"28423","owners":[],"pid":{"revision_id":0,"type":"depid","value":"28423"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00028423","sets":["453:456","485:872:1583:1602"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Expression of Bombyx mori Aminopeptidase N on Plasma Membrane of Cultured Insect Cells and Analysis of Its Receptor Function for Insecticidal Cry Toxins"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"81","bibliographicPageStart":"73","bibliographicVolumeNumber":"60","bibliographic_titles":[{"bibliographic_title":"新潟大学農学部研究報告"},{"bibliographic_title":"新潟大学農学部研究報告","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"カイコガ（Bombyx mori）幼虫の中腸上皮細胞アピカル膜上のブラシ状膜（Brush Border Membrane；BBM）に存在するタンパク質のアミノペプチダーゼN1（BmAPN1）は、鱗翅目昆虫を特異的に殺す毒素Cry1Aa の受容体と考えられているが、その確証は未だ示されていない。BmAPN1が毒性発揮へ関与するかを確認するために、BmAPN1を培養昆虫細胞膜に発現させCry1Aa との相互作用を調査した。昆虫培養細胞株のHigh Five 細胞を用いたバキュロウイルス発現系を構築した。組換ウイルスの感染により、感染処理から48時間後には110 kDa のBmAPN1が発現し細胞膜上に提示された。アミノペプチダーゼ活性は非感染細胞の10倍あり、BmAPN1の活性が保持されていることが示された。High Five 細胞に発現したBmAPN1の分子サイズは、BBM 上に存在するBmAPN1と同程度で、細胞膜上に存在した。組換BmAPN1が活性を持ちしかも細胞膜上に提示されたことで、この発現システムは、アミノペプチダーゼとCry1Aa との相互作用を解析する上で非常に役立つと期待された。 Aminopeptidase N(APN) localizing on the apical membrane of midgut epithelial cells is one of the plausible candidate of the receptor proteins for Bacillus thuringiensis Cry1Aa toxin which specifically kills lepidopteran insects. However, comprehensive evidences to address above possibility have not been established yet. We tried to express the APN1, BmAPN1, onto plasma membrane of cultured insect cells to investigate the interaction with Cry1Aa. Gene expression system was constructed with High five cultured insect cells and baculovirus expression system. On 48 h after the infection of the cells with gene modified baculovirus, 110 kDa BmAPN1 was expressed on the cell membranes. Aminopeptidase activity was ten times higher than that of non-infected cultured cells. The recombinant BmAPN1 was the same size as native one localizing on the brush border membrane of midgut epithelial cells. The transformed cells expressing BmAPN1 is a good system for the investigation of interaction between APN and Cry1Aa toxin.","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"163544","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Noguchi, Rieko"}]},{"nameIdentifiers":[{"nameIdentifier":"163545","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Ishikawa, Toshiki"}]},{"nameIdentifiers":[{"nameIdentifier":"163546","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Haginoya, Kohsuke"}]},{"nameIdentifiers":[{"nameIdentifier":"163547","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Shitomi, Yasuyuki"}]},{"nameIdentifiers":[{"nameIdentifier":"163548","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Sato, Ryoichi"}]},{"nameIdentifiers":[{"nameIdentifier":"163549","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Hayakawa, Tohru"}]},{"nameIdentifiers":[{"nameIdentifier":"163550","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Hori, Hidetaka"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟大学農学部"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00183393","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03858634","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"野口, 理恵子"}],"nameIdentifiers":[{"nameIdentifier":"163537","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"石川, 寿樹"}],"nameIdentifiers":[{"nameIdentifier":"163538","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"萩野谷, 功輔"}],"nameIdentifiers":[{"nameIdentifier":"163539","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"蔀, 泰幸"}],"nameIdentifiers":[{"nameIdentifier":"163540","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐藤, 令一"}],"nameIdentifiers":[{"nameIdentifier":"163541","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"早川, 徹"}],"nameIdentifiers":[{"nameIdentifier":"163542","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"堀, 秀隆"}],"nameIdentifiers":[{"nameIdentifier":"163543","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-20"}],"displaytype":"detail","filename":"1_0011.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"1_0011.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/28423/files/1_0011.pdf"},"version_id":"9a6d23a1-bd84-4781-acff-c7e38eb17ec7"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"アミノペプチダーゼN","subitem_subject_scheme":"Other"},{"subitem_subject":"Bacillus thuringiensis","subitem_subject_scheme":"Other"},{"subitem_subject":"バキュロウイルス","subitem_subject_scheme":"Other"},{"subitem_subject":"Bombyx mori","subitem_subject_scheme":"Other"},{"subitem_subject":"殺虫性タンパク","subitem_subject_scheme":"Other"},{"subitem_subject":"Aminopeptidase N","subitem_subject_scheme":"Other"},{"subitem_subject":"Bacillus thuringiensis","subitem_subject_scheme":"Other"},{"subitem_subject":"Baculovirus","subitem_subject_scheme":"Other"},{"subitem_subject":"Bombyx mori","subitem_subject_scheme":"Other"},{"subitem_subject":"insecticidal proteins","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"カイコアミノペプチダーゼNの培養昆虫細胞膜への発現提示と殺虫性毒素Cry トキシン受容体機能の解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"カイコアミノペプチダーゼNの培養昆虫細胞膜への発現提示と殺虫性毒素Cry トキシン受容体機能の解析"},{"subitem_title":"カイコアミノペプチダーゼNの培養昆虫細胞膜への発現提示と殺虫性毒素Cry トキシン受容体機能の解析","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","1602"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-10-12"},"publish_date":"2007-10-12","publish_status":"0","recid":"28423","relation_version_is_last":true,"title":["カイコアミノペプチダーゼNの培養昆虫細胞膜への発現提示と殺虫性毒素Cry トキシン受容体機能の解析"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:58:28.580796+00:00"}