{"created":"2021-03-01T06:34:13.179914+00:00","id":28413,"links":{},"metadata":{"_buckets":{"deposit":"53c993f6-e4c7-4160-ac48-685cb3ff687a"},"_deposit":{"id":"28413","owners":[],"pid":{"revision_id":0,"type":"depid","value":"28413"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00028413","sets":["453:456","485:872:1583:1602"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"土壌微生物によるs －トリアジン系除草剤の分解とその原位置バイオレメディエーションへの応用"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"7","bibliographicPageStart":"1","bibliographicVolumeNumber":"60","bibliographic_titles":[{"bibliographic_title":"新潟大学農学部研究報告"},{"bibliographic_title":"新潟大学農学部研究報告","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Moderately persistent s-triazine compounds are widely used in agriculture for the control of various weeds. In thisreview, microbial degradation of chloro- and　methylthio-s-triazines and a recent attempt to utilize isolated simazine-degrading bacteria for in situ bioremediation are described. As microorganisms capable of degrading chloro-s-triazines, atrazinedegrading Pseudomonas sp. strain ADP has been studied most extensively. Dechlorination of the s-triazine ring by the strain is mediated by atrazine chlorohydrolase (AtzA) and the subsequent degradation is associated with AtzBCDEF. A novel simazine-degrading β-proteobacterium strain CDB21 isolated by the author's group also possessed entire-set of genes of these enzymes (atzABCDEF). However, while atzA is located between ORF30 and atzB in strain ADP, ORF30 fused with atzB in strain CDB21. This indicates that localization of atzA in CDB21 differs from that in the pseudomonad, meaning that genetic variations regarding the degradation genes exist among microorganisms capable of degrading chloro-s-triazines. It is known that methylthio-s-triazines can be transformed to the corresponding hydroxy analogues via sulfur oxidation in aerobic and flooded soil. Recently, two types of bacteria that engage the process were isolated. One is Bacillus cereus strain JUN7 that can degrade methylthio-s-triazines to give the methylsulfinyl and hydroxy analogues under nutrient-rich conditions such as those with the Luria-Bertani medium. The other is Rhodococcus sp. strain FJ1117YT that is able to degrade methylthios-triazines supplied as the sole sulfur source. These strains were unable to degrade chloro-s-triazines. No gene encoding AtzA or TrzN (triazine hydrolase) was found in these strains, suggesting that they may have another metabolic system. Using a special charcoal material enriched with a simazine-degrading bacterial consortium involving strain CDB21, in situ bioremediation study was conducted in a golf course, where simazine is routinely applied for preservation of turf. As a result, the material was effective for preventing penetration of simazine into subsoils and aquatic environments nearby for approximately 2 years. This result suggests that use of adequate habitat materials is preferable to maintain population and activity of inoculant bacteria applied to soil environment.","subitem_description_type":"Abstract"},{"subitem_description":"環境中で中度の残留性が認められているs-triazine 化合物は、除草剤として農業上広く利用されている。本総説では、塩素化及びメチルチオ化s-triazine 化合物の微生物分解に関するこれまでの知見を述べると共に、単離したsimazine 分解菌を利用した原位置バイオレメディエーションの実施例について紹介する。塩素化s-triazine 化合物の分解微生物としてはatrazine 分解菌として単離されたPseudomonas sp. ADP 株が著名で、最も仔細に研究されている。ADP 株では、まずatrazine chlorohydrolase（AtzA）による脱塩素を初発反応として、その後AtzBCDEF が順に関与して無機化に至る。これらの酵素遺伝子群の全部あるいは一部を有する塩素化s-triazine 分解菌の単離例は多く、我々のグループが単離した新規simazine 分解菌CDB21株でもatzABCDEF 遺伝子のすべてが見つかった。しかし、ADP 株ではatzA がORF30とatzB の間に位置しているのに対して、CDB21株ではORF30がatzB とフューズしており、塩素化s-triazine 分解遺伝子群の構造に多型があることが明らかになった。メチルチオ化s-triazine 化合物では、好気及び湛水のいずれの条件においてもメチルチオ基のS 酸化を解して分解が進むことが古くから知られていたが、最近ようやくそのプロセスを担う微生物、Bacillus cereus JUN7株とRhodococcus sp. FJ1117YT 株が単離された。JUN7株はLuria-Bertani 培地のような富栄養培地中で、またFJ1117YT 株はメチルチオ化s-triazine 化合物を唯一のS 源として与えた無機培地中でメチルチオ基をS 酸化し、最終的に脱メチルチオ化物（水酸化物）を生成した。両株とも塩素化s-triazine 化合物を分解せず、またatzA やtrzN（triazine hydrolase 遺伝子）を持たないことから、未知の分解機構の存在が示唆される。また、CDB21株を含むsimazine 分解複合微生物系を集積させた特殊な木質炭化素材を用いて、芝草保全の為に定期的にsimazine が散布されるゴルフ場において原位置バイオレメディエーション実証実験を行ったところ、本資材は約２年間に渡ってsimazine の下方浸透防止に有効であった。この結果は、土壌環境へ外部から微生物を導入する際にはその住処となりうる適当な資材の併用が好ましいことを示している。","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"163454","nameIdentifierScheme":"WEKO"}],"names":[{"name":"原田, 直樹"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟大学農学部"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00183393","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03858634","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Harada, Naoki"}],"nameIdentifiers":[{"nameIdentifier":"163453","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-20"}],"displaytype":"detail","filename":"1_0001.pdf","filesize":[{"value":"944.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"1_0001.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/28413/files/1_0001.pdf"},"version_id":"94290799-3e5c-4563-879f-f76406ff7223"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"biodegradation","subitem_subject_scheme":"Other"},{"subitem_subject":"in situ bioremediation","subitem_subject_scheme":"Other"},{"subitem_subject":"simazine","subitem_subject_scheme":"Other"},{"subitem_subject":"simetryn","subitem_subject_scheme":"Other"},{"subitem_subject":"s-triazine","subitem_subject_scheme":"Other"},{"subitem_subject":"微生物分解","subitem_subject_scheme":"Other"},{"subitem_subject":"原位置バイオレメディエーション","subitem_subject_scheme":"Other"},{"subitem_subject":"シマジン","subitem_subject_scheme":"Other"},{"subitem_subject":"シメトリン","subitem_subject_scheme":"Other"},{"subitem_subject":"s －トリアジン","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Biodegradation of s-Triazine Herbicides by Soil Microorganisms and Their Application for In Situ Bioremediation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Biodegradation of s-Triazine Herbicides by Soil Microorganisms and Their Application for In Situ Bioremediation"},{"subitem_title":"Biodegradation of s-Triazine Herbicides by Soil Microorganisms and Their Application for In Situ Bioremediation","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","1602"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-10-12"},"publish_date":"2007-10-12","publish_status":"0","recid":"28413","relation_version_is_last":true,"title":["Biodegradation of s-Triazine Herbicides by Soil Microorganisms and Their Application for In Situ Bioremediation"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:58:29.119482+00:00"}