{"_buckets": {"deposit": "d11fa0af-62e1-4180-8ee1-da718563a5e2"}, "_deposit": {"id": "28406", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28406"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028406"}, "item_7_alternative_title_1": {"attribute_name": "\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "Effect of Methanol Concentration and Temperature on Retention Time of Abscisic Acid, Indole-3-acetic Acid and Zeatin"}]}, "item_7_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2008-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "122", "bibliographicPageStart": "119", "bibliographicVolumeNumber": "60", "bibliographic_titles": [{"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a"}, {"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "HPLC\u306b\u3088\u3063\u3066\u690d\u7269\u30db\u30eb\u30e2\u30f3\u3092\u52b9\u7387\u7684\u306b\u7cbe\u88fd\u3059\u308b\u305f\u3081\u306b\u3001ODS\u30bf\u30a4\u30d7\u306e\u30ab\u30e9\u30e0\u306b\u304a\u3051\u308b\u30a2\u30d6\u30b7\u30b8\u30f3\u9178\uff08ABA\uff09\u3001\u30c8\u30e9\u30f3\u30b9-\u30a2\u30d6\u30b7\u30b8\u30f3\u9178\uff08t-ABA\uff09\u30a4\u30f3\u30c9\u30fc\u30eb-3-\u9162\u9178\uff08IAA\uff09\u304a\u3088\u3073\u30bc\u30a2\u30c1\u30f3\uff08Z\uff09\u306e\u4fdd\u6301\u6642\u9593\u3092\u6e2c\u5b9a\u3057\u3001\u30e1\u30bf\u30ce\u30fc\u30eb\u6fc3\u5ea6\u307e\u305f\u306f\u30ab\u30e9\u30e0\u6e29\u5ea6\u304c\u3053\u308c\u3089\u306e\u4fdd\u6301\u6642\u9593\u306b\u304a\u3088\u307c\u3059\u5f71\u97ff\u3092\u793a\u3057\u305f\u300230\u2103\u306e\u30ab\u30e9\u30e0\u6e29\u5ea6\u306735\uff05MeOH\u306e\u6eb6\u96e2\u6db2\u3067\u306e\u5206\u6790\u6761\u4ef6\u306b\u304a\u3044\u3066\u30014\u3064\u306e\u6a19\u6e96\u7269\u8cea\u306fZ\uff088.0\u5206\uff09\u3001IAA\uff0844\u5206\uff09\u3001t-ABA\uff0860\u5206\uff09\u3001ABA\uff0893\u5206\uff09\u306e\u9806\u306b\u6eb6\u51fa\u3057\u305f\u300235\uff05MeOH\u3067\u30ab\u30e9\u30e0\u6e29\u5ea6\u309230\u2103\u304b\u308950\u2103\u306b\u4e0a\u6607\u3055\u305b\u305f\u3068\u304d\u30014\u3064\u306e\u6a19\u6e96\u7269\u8cea\u306e\u6eb6\u51fa\u306e\u9806\u5e8f\u306f\u5909\u5316\u3057\u306a\u304b\u3063\u305f\u304c\u3001\u6e29\u5ea6\u304c\u5897\u52a0\u3059\u308b\u306b\u3064\u308c\u3066ABA\u3001t-ABA\u304a\u3088\u3073IAA\u304c\u65e9\u304f\u6eb6\u96e2\u3057\u305f\u3002\u3057\u304b\u3057\u306a\u304c\u3089\u3001Z\u306e\u4fdd\u6301\u6642\u9593\u306f\u30ab\u30e9\u30e0\u6e29\u5ea6\u304c30\u2103\u304b\u308950\u2103\u306b\u5897\u52a0\u3057\u3066\u3082\u307b\u3068\u3093\u3069\u5909\u5316\u3057\u306a\u304b\u3063\u305f\u300245%MeOH\u304a\u3088\u307350\uff05MeOH\u306e\u6eb6\u96e2\u6db2\u306b\u304a\u3044\u3066\u3001\u6a19\u6e96\u7269\u8cea\u306e\u6eb6\u51fa\u306e\u9806\u5e8f\uff08Z\u2192IAA \u2192t-ABA\u2192ABA\uff09\u306f35\uff05MeOH\u3068\u540c\u69d8\u3067\u3042\u308a\u3001MeOH\u6fc3\u5ea6\u304c\u4e0a\u6607\u3059\u308b\u306b\u3064\u308c\u3066Z\u3001ABA\u3001t-ABA\u304a\u3088\u3073IAA\u304c\u65e9\u304f\u6eb6\u96e2\u3057\u305f\u3002\u6a19\u6e96\u7269\u8cea\u306e\u4fdd\u6301\u6642\u9593\u306f\u30ab\u30e9\u30e0\u6e29\u5ea6\u3068\u9ad8\u3044\u76f8\u95a2\u304c\u3042\u308a\u3001\u5168\u6a19\u6e96\u7269\u8cea\u306b\u304a\u3051\u308b\u56de\u5e30\u76f4\u7dda\u306eR2\u5024\u306f\u5168\u3066\u306e\u30e1\u30bf\u30ce\u30fc\u30eb\u6fc3\u5ea6\u3067\u9ad8\u304b\u3063\u305f\u3002\u5168\u6a19\u6e96\u7269\u8cea\u306b\u304a\u3044\u3066\u3001MeOH\u6fc3\u5ea6\u304c\u9ad8\u304f\u306a\u308b\u3068\u3001\u4fdd\u6301\u6642\u9593\u3068\u30ab\u30e9\u30e0\u6e29\u5ea6\u306e\u9593\u306e\u56de\u5e30\u76f4\u7dda\u306e\u50be\u304d\u306f\u6e1b\u5c11\u3057\u305f\u3002\u3053\u308c\u3089\u306e\u7d50\u679c\u304b\u3089\u3001HPLC\u30ab\u30e9\u30e0\u306b\u304a\u3051\u308b\u690d\u7269\u30db\u30eb\u30e2\u30f3\u306e\u4fdd\u6301\u6642\u9593\u3068\u30ab\u30e9\u30e0\u6e29\u5ea6\u304a\u3088\u3073MeOH\u6fc3\u5ea6\u306e\u7d44\u307f\u5408\u308f\u305b\u306e\u95a2\u4fc2\u304c\u793a\u3055\u308c\u305f\u3002These results showed the relationship between the retention times of plant hormones in a HPLC column and combination of column temperature and MeOH concentration. In all standard substances, as MeOH concentration was increased, inclination values of liner regression between retention time and column temperature were decreased. The retention times of standard substances have a correlation with column temperature, and R2 values of liner regression in all standard substances were high in all MeOH concentrations. In the eluent of 40% and 50% MeOH, the orders of retention times of authentic standards (Z\u2192IAA\u2192t-ABA\u2192ABA) were identical to 35% MeOH, as MeOH concentration increased, Z, ABA, t-ABA and IAA were eluted rapidly. However, the retention time of Z has hardly changed when the column temperature was increased from 30\u2103 to 50\u2103. When the column temperature was increased from 30\u2103 to 50\u2103 with 35% MeOH, the order of elution of four standard substances did not change, but as the column temperature increased, ABA, t-ABA and IAA were eluted rapidly. In the analysis condition with eluent of 35 % methanol (MeOH) and column temperature at 30\u2103, four authentic standards were eluted in order of Z (8.0 min), IAA (44 min), t-ABA (60 min), ABA (93 min). The retention times of Abscisic acid (ABA), trans-abscisic acid (t-ABA), indole-3-acetic acid (IAA) and zeatin (Z) in a column of ODS type were determined to refine plant hormones efficiently by a HPLC (high performance liquid chromatography), which showed the effects of methanol concentration or temperature on retention times of these standards.", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "163419", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Ohta, Yuuki"}]}, {"nameIdentifiers": [{"nameIdentifier": "163420", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Murata, Yoshiaki"}]}, {"nameIdentifiers": [{"nameIdentifier": "163421", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Chino, Shuji"}]}, {"nameIdentifiers": [{"nameIdentifier": "68", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Kojima, Kiyohide"}]}]}, 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