{"created":"2021-03-01T06:34:11.985615+00:00","id":28395,"links":{},"metadata":{"_buckets":{"deposit":"48c9f8a3-4843-4d31-9d01-0f085b9b4c73"},"_deposit":{"id":"28395","owners":[],"pid":{"revision_id":0,"type":"depid","value":"28395"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00028395","sets":["453:456","485:872:1583:1600"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"ラット好塩基球様RBL-2H3細胞におけるγ- アミノ酪酸（GABA）のヒスタミン遊離抑制作用"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"51","bibliographicPageStart":"47","bibliographicVolumeNumber":"61","bibliographic_titles":[{"bibliographic_title":"新潟大学農学部研究報告"},{"bibliographic_title":"新潟大学農学部研究報告","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Basophils and mast cells play a crucial role in immediate allergic reactions. These cells release various infl ammatory mediators including histamine in response to binding of antigen to its specifi c IgE bound to the high affi nity IgE receptor on the cell surface. In the present study, we examined the influence of γ-aminobutyric acid (GABA) on histamine release in rat basophilic leukemia RBL-2H3 cells. After sensitization with anti-dinitrophenyl (DNP) IgE, challenge of RBL-2H3 cells with DNP-conjugated bovine serum albumin (DNP-BSA) caused a release of histamine in the cell suspension. The IgEmediated histamine release was suppressed by GABA, with about 60% inhibition at 100 μM. In the same condition, baclofen, a GABA_B receptor agonist, also inhibited histamine release. At a concentration of 100μM, the inhibition rate of baclofen was approximate 80% of histamine release induced by DNP-BSA stimulation. In addition, these inhibitory eff ects of GABA and baclofen were attenuated by the addition of CGP35348, a GABA_B receptor antagonist. These results suggest that GABA suppresses degranulation in basophils and mast cells via GABA_B receptor.","subitem_description_type":"Abstract"},{"subitem_description":"好塩基球やマスト細胞は、即時型アレルギー反応において重要な役割を担う。これらの細胞は、細胞表面上の高親和性IgEレセプターに結合した特異的IgE にアレルゲンが結合すると、脱顆粒によりヒスタミンなどの様々な炎症性メディエーターを遊離する。本研究では、ラット好塩基球様RBL-2H3細胞を用い、ヒスタミン遊離に対するγ- アミノ酪酸（GABA）の影響を検討した。RBL-2H3細胞は、抗ジニトロフェニルIgE（DNP-IgE）により感作した後、抗原としてジニトロフェニル化ウシ血清アルブミン（DNP-BSA）を添加することによりヒスタミンを遊離した。このIgE を介したヒスタミン遊離は、GABA により抑制され、濃度100μM において約60％の抑制率を示した。同様の実験条件において、GABA_Bレセプター作動薬であるバクロフェンもまた、ヒスタミン遊離を抑制した。濃度100 μM において、バクロフェンの抑制率はDNP-BSA によるヒスタミン遊離の約80％に達した。さらに、GABAおよびバクロフェンのヒスタミン遊離抑制作用は、GABA_Bレセプター阻害剤であるCGP35348により減弱した。これらの結果は、GABAがGABA_Bレセプターを介し、マスト細胞および好塩基球のヒスタミン遊離を抑制することを示唆する。","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"163351","nameIdentifierScheme":"WEKO"}],"names":[{"name":"堀, あゆみ"}]},{"nameIdentifiers":[{"nameIdentifier":"163352","nameIdentifierScheme":"WEKO"}],"names":[{"name":"原, 崇"}]},{"nameIdentifiers":[{"nameIdentifier":"163353","nameIdentifierScheme":"WEKO"}],"names":[{"name":"本間, 香織"}]},{"nameIdentifiers":[{"nameIdentifier":"163354","nameIdentifierScheme":"WEKO"}],"names":[{"name":"城, 斗志夫"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟大学農学部"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00183393","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03858634","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hori, Ayumi"}],"nameIdentifiers":[{"nameIdentifier":"163347","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hara, Takashi"}],"nameIdentifiers":[{"nameIdentifier":"163348","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Honma, Kaori"}],"nameIdentifiers":[{"nameIdentifier":"163349","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Joh, Toshio"}],"nameIdentifiers":[{"nameIdentifier":"163350","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-20"}],"displaytype":"detail","filename":"61(1)_47-51.pdf","filesize":[{"value":"205.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"61(1)_47-51.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/28395/files/61(1)_47-51.pdf"},"version_id":"b5f5c22a-97c8-4479-b242-e01132c4e981"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"degranulation","subitem_subject_scheme":"Other"},{"subitem_subject":"γ-aminobutyric acid (GABA)","subitem_subject_scheme":"Other"},{"subitem_subject":"GABAB receptor","subitem_subject_scheme":"Other"},{"subitem_subject":"histamine","subitem_subject_scheme":"Other"},{"subitem_subject":"RBL-2H3 cells","subitem_subject_scheme":"Other"},{"subitem_subject":"脱顆粒","subitem_subject_scheme":"Other"},{"subitem_subject":"γ- アミノ酪酸（GABA）","subitem_subject_scheme":"Other"},{"subitem_subject":"GABA_Bレセプター","subitem_subject_scheme":"Other"},{"subitem_subject":"ヒスタミン","subitem_subject_scheme":"Other"},{"subitem_subject":"RBL-2H3細胞","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Suppressive Effect of γ-Aminobutyric Acid (GABA) on Histamine Release in Rat Basophilic RBL-2H3 Cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Suppressive Effect of γ-Aminobutyric Acid (GABA) on Histamine Release in Rat Basophilic RBL-2H3 Cells"},{"subitem_title":"Suppressive Effect of γ-Aminobutyric Acid (GABA) on Histamine Release in Rat Basophilic RBL-2H3 Cells","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","1600"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-07-02"},"publish_date":"2010-07-02","publish_status":"0","recid":"28395","relation_version_is_last":true,"title":["Suppressive Effect of γ-Aminobutyric Acid (GABA) on Histamine Release in Rat Basophilic RBL-2H3 Cells"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:58:15.231412+00:00"}