{"created":"2021-03-01T06:34:11.919539+00:00","id":28394,"links":{},"metadata":{"_buckets":{"deposit":"53218d59-2d9a-420c-86c9-9b55ce0a7c16"},"_deposit":{"id":"28394","owners":[],"pid":{"revision_id":0,"type":"depid","value":"28394"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00028394","sets":["453:456","485:872:1583:1600"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"形質転換法による高アミロース日本稲の確立"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"45","bibliographicPageStart":"41","bibliographicVolumeNumber":"61","bibliographic_titles":[{"bibliographic_title":"新潟大学農学部研究報告"},{"bibliographic_title":"新潟大学農学部研究報告","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The Wx gene encodes a granule-bound starch synthase I (GBSSI) that plays a key role in the amylose synthesis. Previous study showed that introduction of Wx transgene into null-mutant wx rice (WxR/wx) by direct gene transfer leads to both high- and low- amylose rice. In later generations of several transgenic WxR/wx lines, unstable and silenced endosperm phenotypes appeared. The unstable and silenced phenotypes are repeat-induced gene silencing caused by integration of multicopy transgenes (~11 copies per haploid genome). In order to solve the unstable phenotype emergence, binary vector pWAB was constructed and transferred into null-mutant wx by Agrobacterium-mediated gene transfer. Resultant transgenic rice, WAB/wx lines, carrying low copy number of the transgenes (~3 copies per haploid), showed high-amylose phenotype at the endosperm, and the phenotypes are stably transmitted to next generations.","subitem_description_type":"Abstract"},{"subitem_description":"デンプン顆粒結合型デンプン合成酵素をコードするWx遺伝子はアミロース合成を司る鍵酵素である。これまでの研究から、直接導入法によりwx欠損変異体に外来Wx遺伝子を導入したイネ（WxR/wx）では、高アミロース型と低アミロース型双方の表現型を示した。そのうち複数の系統の後代において、不安定かつ不活性化された表現型が現れた。この原因は、外来遺伝子が多コピー導入されたことにより、反復配列誘導性ジーンサイレンシングが起きたためである。この不安定な表現型の出現を解決するために、バイナリーベクターpWABを構築し、アグロバクテリウム法によりwx欠損変異体に導入した。得られた形質転換イネ、WAB/wx系統では低コピー数の外来遺伝子が確認され、胚乳において高アミロース型であり、その胚乳形質は安定に次世代に遺伝した。","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"163343","nameIdentifierScheme":"WEKO"}],"names":[{"name":"長谷川, 真理"}]},{"nameIdentifiers":[{"nameIdentifier":"163344","nameIdentifierScheme":"WEKO"}],"names":[{"name":"関川, 晶子"}]},{"nameIdentifiers":[{"nameIdentifier":"163345","nameIdentifierScheme":"WEKO"}],"names":[{"name":"丹野, 史典"}]},{"nameIdentifiers":[{"nameIdentifier":"163346","nameIdentifierScheme":"WEKO"}],"names":[{"name":"伊藤, 紀美子"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟大学農学部"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00183393","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03858634","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hasegawa, Mari"}],"nameIdentifiers":[{"nameIdentifier":"163339","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sekikawa, Akiko"}],"nameIdentifiers":[{"nameIdentifier":"163340","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tanno, Fuminori"}],"nameIdentifiers":[{"nameIdentifier":"163341","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Itoh, Kimiko"}],"nameIdentifiers":[{"nameIdentifier":"163342","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-20"}],"displaytype":"detail","filename":"61(1)_41-45.pdf","filesize":[{"value":"195.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"61(1)_41-45.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/28394/files/61(1)_41-45.pdf"},"version_id":"108de772-e9c3-4074-8f90-d18b9040b67e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Wx","subitem_subject_scheme":"Other"},{"subitem_subject":"Granule-bound starch synthase I","subitem_subject_scheme":"Other"},{"subitem_subject":"Amylose","subitem_subject_scheme":"Other"},{"subitem_subject":"Japonica rice","subitem_subject_scheme":"Other"},{"subitem_subject":"デンプン結合型デンプン合成酵素I","subitem_subject_scheme":"Other"},{"subitem_subject":"アミロース","subitem_subject_scheme":"Other"},{"subitem_subject":"日本稲","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Establishment of High-amylose Type Japonica Rice by Agrobacterium-mediated Gene Transfer","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Establishment of High-amylose Type Japonica Rice by Agrobacterium-mediated Gene Transfer"},{"subitem_title":"Establishment of High-amylose Type Japonica Rice by Agrobacterium-mediated Gene Transfer","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","1600"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-07-02"},"publish_date":"2010-07-02","publish_status":"0","recid":"28394","relation_version_is_last":true,"title":["Establishment of High-amylose Type Japonica Rice by Agrobacterium-mediated Gene Transfer"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:58:25.610015+00:00"}