{"_buckets": {"deposit": "53218d59-2d9a-420c-86c9-9b55ce0a7c16"}, "_deposit": {"id": "28394", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28394"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028394", "sets": ["456", "1600"]}, "item_7_alternative_title_1": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "形質転換法による高アミロース日本稲の確立"}]}, "item_7_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2008-09", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "45", "bibliographicPageStart": "41", "bibliographicVolumeNumber": "61", "bibliographic_titles": [{"bibliographic_title": "新潟大学農学部研究報告"}, {"bibliographic_title": "新潟大学農学部研究報告", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "The Wx gene encodes a granule-bound starch synthase I (GBSSI) that plays a key role in the amylose synthesis. Previous study showed that introduction of Wx transgene into null-mutant wx rice (WxR/wx) by direct gene transfer leads to both high- and low- amylose rice. In later generations of several transgenic WxR/wx lines, unstable and silenced endosperm phenotypes appeared. The unstable and silenced phenotypes are repeat-induced gene silencing caused by integration of multicopy transgenes (~11 copies per haploid genome). In order to solve the unstable phenotype emergence, binary vector pWAB was constructed and transferred into null-mutant wx by Agrobacterium-mediated gene transfer. Resultant transgenic rice, WAB/wx lines, carrying low copy number of the transgenes (~3 copies per haploid), showed high-amylose phenotype at the endosperm, and the phenotypes are stably transmitted to next generations.", "subitem_description_type": "Abstract"}, {"subitem_description": "デンプン顆粒結合型デンプン合成酵素をコードするWx遺伝子はアミロース合成を司る鍵酵素である。これまでの研究から、直接導入法によりwx欠損変異体に外来Wx遺伝子を導入したイネ（WxR/wx）では、高アミロース型と低アミロース型双方の表現型を示した。そのうち複数の系統の後代において、不安定かつ不活性化された表現型が現れた。この原因は、外来遺伝子が多コピー導入されたことにより、反復配列誘導性ジーンサイレンシングが起きたためである。この不安定な表現型の出現を解決するために、バイナリーベクターpWABを構築し、アグロバクテリウム法によりwx欠損変異体に導入した。得られた形質転換イネ、WAB/wx系統では低コピー数の外来遺伝子が確認され、胚乳において高アミロース型であり、その胚乳形質は安定に次世代に遺伝した。", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "163343", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "長谷川, 真理"}]}, {"nameIdentifiers": [{"nameIdentifier": "163344", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "関川, 晶子"}]}, {"nameIdentifiers": [{"nameIdentifier": "163345", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "丹野, 史典"}]}, {"nameIdentifiers": [{"nameIdentifier": "163346", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "伊藤, 紀美子"}]}]}, "item_7_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "新潟大学農学部"}]}, "item_7_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00183393", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "03858634", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Hasegawa, Mari"}], "nameIdentifiers": [{"nameIdentifier": "163339", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sekikawa, Akiko"}], "nameIdentifiers": [{"nameIdentifier": "163340", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tanno, Fuminori"}], "nameIdentifiers": [{"nameIdentifier": "163341", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Itoh, Kimiko"}], "nameIdentifiers": [{"nameIdentifier": "163342", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "61(1)_41-45.pdf", "filesize": [{"value": "195.9 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 195900.0, "url": {"label": "61(1)_41-45.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/28394/files/61(1)_41-45.pdf"}, "version_id": "108de772-e9c3-4074-8f90-d18b9040b67e"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Wx", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Granule-bound starch synthase I", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Amylose", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Japonica rice", "subitem_subject_scheme": "Other"}, {"subitem_subject": "デンプン結合型デンプン合成酵素I", "subitem_subject_scheme": "Other"}, {"subitem_subject": "アミロース", "subitem_subject_scheme": "Other"}, {"subitem_subject": "日本稲", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Establishment of High-amylose Type Japonica Rice by Agrobacterium-mediated Gene Transfer", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Establishment of High-amylose Type Japonica Rice by Agrobacterium-mediated Gene Transfer"}, {"subitem_title": "Establishment of High-amylose Type Japonica Rice by Agrobacterium-mediated Gene Transfer", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["456", "1600"], "permalink_uri": "http://hdl.handle.net/10191/13068", "pubdate": {"attribute_name": "公開日", "attribute_value": "2010-07-02"}, "publish_date": "2010-07-02", "publish_status": "0", "recid": "28394", "relation": {}, "relation_version_is_last": true, "title": ["Establishment of High-amylose Type Japonica Rice by Agrobacterium-mediated Gene Transfer"], "weko_shared_id": null}