{"created":"2021-03-01T06:34:11.657232+00:00","id":28390,"links":{},"metadata":{"_buckets":{"deposit":"239b2cc6-9d70-4d36-a125-af807c8c2799"},"_deposit":{"id":"28390","owners":[],"pid":{"revision_id":0,"type":"depid","value":"28390"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00028390","sets":["453:456","485:872:1583:1600"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Polar Transport and Metabolism of Indole-3-acetic Acid in Tomato Stem"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"15","bibliographicPageStart":"11","bibliographicVolumeNumber":"61","bibliographic_titles":[{"bibliographic_title":"新潟大学農学部研究報告"},{"bibliographic_title":"新潟大学農学部研究報告","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"トマトの茎におけるインドール-3- 酢酸（Indole-3-acetic acid; IAA）の極性輸送および代謝を、[2-14C] IAA を用いて調査した。極性輸送は、茎の上部および下部の長さ2.5cm の切片を用いて測定した。IAA の輸送量は茎の下部（0.8 pmol/ 寒天/cm2）に比べ、上部では約4-7倍（3.0-5.8 pmol/ 寒天/cm2）であり、さらに上部では4.5時間以降、急激に増加した（1.5-2倍）。維管束などの輸送経路と考えられる量を除いて計算した輸速速度は、茎の上部切片で、1時間当たり0.67cm となり、一般的な輸送速度の範囲内であった。しかし、下部切片の輸送速度は算出できなかった。代謝は、茎の中央部の長さ10cm の切片を用いて調査した。[2-14C]IAAを24時間施与した切片の先端部、上部、中央部および下部の中で、放射能量は、先端部が最も多く、下方に輸送するにしたがって減少した。しかし、下部ではわずかに増加した。この放射能量の増加の原因は、極性輸送の結果として蓄積した、もしくは維管束などのほかの輸送経路の存在が考えられる。液体シンチレーションカウンターの結果から、IAAに相当する保持時間にピークは検出されなかったが、15分以内に多くの代謝物と推定されるピークが検出された。しかし、トマトの茎切片を用いた抽出・精製過程中のIAAの分解量を調べる実験では、代謝物と推定されるピークがIAAに相当するピークよりも多く検出された。したがって、濃縮中の加熱などの影響で分解が起こり、すべての[2-14C] IAAが代謝したと考えられる。","subitem_description_type":"Abstract"},{"subitem_description":"Polar transport and metabolism of indole-3-acetic acid in tomato stems were investigated using [2-14C] IAA. Polar transport was measured using the 2.5 cm sections of the upper part and the lower part of stems. The contents of IAA transport were about 4-7 times in the upper sections (3.0-5.8 pmol/ager/cm2) than in the lower sections (0.8 pmol/ager/cm2) in the stems and moreover increased rapidly after 4.5 hours. (1.5-2 times). The transport speed which was calculated except for the content through the traffi c channel of the vascular bundle was 0.67 cm per hour in the upper section of the stem. It was within the transport speed in general. However, the transport speed of the ower section could not be calculated. Metabolism was investigated using the 10 cm section of the middle of the stem. In the tip part, the upper part, the middle part and the lower part of the section treated with [2-14C] IAA for 24 hour, the radioactivity content was highest in the tip part and decreased to the lower parts. However, it increased a little in the lower part. As the cause of the increase of the radioactivity content, it was thought of the accumulated as the result of the polar transport or the existence of the other traffi c channel (e.g. the vascular bundle). As the result from the liquid scintillation counter, the peak which corresponded to retention time of IAA (IAA correspond peak) wasn't detected, but a lot of peaks which were assumed as the metabolites of IAA (Metabolite peaks) were detected within 15 minutes. However, in the experiment to examine the decomposition content of IAA in the process of the extraction and purifi cation using the tomato section, the content of Metabolite peaks was higher than IAA correspond peak. Therefore, it was thought that the infl uences such as the heating in the concentration process caused the resolution and that [2-14C] IAA changed to metabolite completely.","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"163323","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Ohta, Yuuki"}]},{"nameIdentifiers":[{"nameIdentifier":"163324","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Ikarashi, Ikumi"}]},{"nameIdentifiers":[{"nameIdentifier":"163325","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Chino, Shuji"}]},{"nameIdentifiers":[{"nameIdentifier":"68","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Kojima, Kiyohide"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟大学農学部"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00183393","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03858634","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"太田, 祐樹"}],"nameIdentifiers":[{"nameIdentifier":"163319","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"五十嵐, 育美"}],"nameIdentifiers":[{"nameIdentifier":"163320","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"知野, 秀次"}],"nameIdentifiers":[{"nameIdentifier":"163321","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"児島, 清秀"}],"nameIdentifiers":[{"nameIdentifier":"68","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-20"}],"displaytype":"detail","filename":"61(1)_11-15.pdf","filesize":[{"value":"659.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"61(1)_11-15.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/28390/files/61(1)_11-15.pdf"},"version_id":"fa4f1bf7-c7be-4fb7-b97f-2e754c583526"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"トマト","subitem_subject_scheme":"Other"},{"subitem_subject":"極性輸送","subitem_subject_scheme":"Other"},{"subitem_subject":"代謝","subitem_subject_scheme":"Other"},{"subitem_subject":"インドール-3-酢酸","subitem_subject_scheme":"Other"},{"subitem_subject":"[2-14C]IAA","subitem_subject_scheme":"Other"},{"subitem_subject":"Metabolism","subitem_subject_scheme":"Other"},{"subitem_subject":"Polar transport","subitem_subject_scheme":"Other"},{"subitem_subject":"Tomato","subitem_subject_scheme":"Other"},{"subitem_subject":"Indole-3-acetic acid","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"トマトの茎におけるインドール-3-酢酸の極性輸送と代謝","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"トマトの茎におけるインドール-3-酢酸の極性輸送と代謝"},{"subitem_title":"トマトの茎におけるインドール-3-酢酸の極性輸送と代謝","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","1600"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-07-02"},"publish_date":"2010-07-02","publish_status":"0","recid":"28390","relation_version_is_last":true,"title":["トマトの茎におけるインドール-3-酢酸の極性輸送と代謝"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:58:25.486784+00:00"}