{"created":"2021-03-01T06:34:11.054935+00:00","id":28381,"links":{},"metadata":{"_buckets":{"deposit":"ea2b2751-d599-40c0-ba5c-830297af6cbd"},"_deposit":{"id":"28381","owners":[],"pid":{"revision_id":0,"type":"depid","value":"28381"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00028381"},"item_7_alternative_title_1":{"attribute_name":"\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb","attribute_value_mlt":[{"subitem_alternative_title":"\u57f9\u990a\u3057\u305f\u30de\u30a6\u30b9\u306e\u88f8\u5316\u5375\u6bcd\u7d30\u80de\u306b\u304a\u3051\u308b\u56f2\u5375\u8154\u306e\u5927\u304d\u3055\u3068\u30d2\u30a2\u30eb\u30ed\u30ca\u30f3\u5408\u6210\u306e\u53ef\u80fd\u6027"}]},"item_7_biblio_info_6":{"attribute_name":"\u66f8\u8a8c\u60c5\u5831","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"133","bibliographicPageStart":"127","bibliographicVolumeNumber":"61","bibliographic_titles":[{"bibliographic_title":"\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a"},{"bibliographic_title":"\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"\u6284\u9332","attribute_value_mlt":[{"subitem_description":"The size of perivitelline space was measured in mouse oocytes cultured without cumulus cells (DOs) and compared with that in oocytes cultured with cumulus cells. In addition, the size of perivitelline space of DOs cultured in the medium containing 4-methylumbelliferone (MU), an inhibitor of hyaluronan synthase, was observed to examine whether oocytes are involved in the synthesis and secretion of hyaluronan which plays a role in the enlargement of perivitelline space. Of oocytes cultured with and without cumulus cells for 14hrs, 96.7% (29/30) and 93.5% (29/31) held 1st polar bodies in their perivitelline space, respectively. The percentages of oocytes with 1st polar bodies in the perivitelline space (maturation rates) did not differ between those cultured with and without cumulus cells. The mean size of perivitelline space in oocytes cultured with cumulus cells (5.40\u03bcm) did not differ from that (5.08\u03bcm) of DOs. When DOs were cultured in the medium containing 0.25mM MU, 88.2% of the oocytes held 1st polar bodies in the perivitelline space, showing no difference from the percentage (85.7%) of control DOs cultured in the medium without MU. The mean size of perivitelline space in DOs treated with 0.25mM MU was 3.58\u03bcm, which was significantly smaller than 4.65\u03bcm in the control DOs. When DOs were cultured in the media containing 0.25mM MU and hyaluronan at 0.05, 0.25 or 0.50 mg/ml, 86.9 to 92.8% of the DOs held 1st polar bodies in the perivitelline space, showing no difference from the percentage (88.2%) of the DOs cultured in the medium with only 0.25mM MU. The mean sizes of perivitelline space in DOs cultured in the media containing 0.25mM MU and hyaluronan at 0.05 or 0.50mg/ml were 4.24 and 4.29\u03bcm, respectively, showing no difference from the size (3.85\u03bcm) of the DOs cultured in the medium containing only MU. However, the mean size of perivitelline space in DOs cultured in the medium containing 0.25mM MU and 0.25mg/ml hyaluronan was 4.67\u03bcm, which was significantly larger than that of the DOs cultured in the medium containing only MU. These findings strongly suggested that hyaluronan involved in the enlargement of perivitelline space of oocytes is synthesized and secreted by the oocytes themselves.","subitem_description_type":"Abstract"},{"subitem_description":"\u57f9\u990a\u3057\u305f\u30de\u30a6\u30b9\u306e\u88f8\u5316\u5375\u6bcd\u7d30\u80de\uff08DO\uff09\u306b\u3064\u3044\u3066\u3001\u56f2\u5375\u8154\u306e\u5927\u304d\u3055\u3092\u8a08\u6e2c\u3057\u3066\u5375\u4e18\u5375\u6bcd\u7d30\u80de\u8907\u5408\u4f53\uff08COC\uff09\u306e\u5375\u6bcd\u7d30\u80de\u306e\u3082\u306e\u3068\u6bd4\u8f03\u3059\u308b\u3068\u3068\u3082\u306b\u3001\u30d2\u30a2\u30eb\u30ed\u30ca\u30f3\u5408\u6210\u3092\u963b\u5bb3\u3059\u308b\u4f5c\u7528\u306e\u3042\u308b4-\u30e1\u30c1\u30eb\u30a6\u30f3\u30d9\u30ea\u30d5\u30a7\u30ed\u30f3\uff08MU\uff09\u3067\u51e6\u7f6e\u3057\u305fDO\u306e\u56f2\u5375\u8154\u306e\u5927\u304d\u3055\u3092\u89b3\u5bdf\u3057\u3001\u56f2\u5375\u8154\u306e\u62e1\u5927\u306b\u5f79\u5272\u3092\u679c\u305f\u3059\u30d2\u30a2\u30eb\u30ed\u30ca\u30f3\u306e\u5408\u6210\u3068\u5206\u6ccc\u306b\u5375\u6bcd\u7d30\u80de\u304c\u95a2\u4e0e\u3057\u3066\u3044\u308b\u306e\u304b\u3069\u3046\u304b\u3092\u691c\u8a0e\u3057\u305f\u3002\u63a1\u53d6\u3057\u305fDO\u3068COC\u309214\u6642\u9593\u57f9\u990a\u3057\u305f\u3068\u3053\u308d\u3001\u5375\u6bcd\u7d30\u80de\u306e\u6210\u719f\u7387\u306f93.5\u304a\u3088\u307396.7\uff05\u3067\u3042\u308a\u3001\u4e21\u8005\u3067\u6709\u610f\u306a\u5dee\u306f\u306a\u304b\u3063\u305f\u3002DO\u306e\u56f2\u5375\u8154\u306f5.08\u03bcm 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production","subitem_subject_scheme":"Other"},{"subitem_subject":"mouse","subitem_subject_scheme":"Other"},{"subitem_subject":"oocyte with cumulus cell","subitem_subject_scheme":"Other"},{"subitem_subject":"size of perivitelline space","subitem_subject_scheme":"Other"},{"subitem_subject":"\u56f2\u5375\u8154\u306e\u5927\u304d\u3055","subitem_subject_scheme":"Other"},{"subitem_subject":"\u30d2\u30a2\u30eb\u30ed\u30ca\u30f3\u5408\u6210","subitem_subject_scheme":"Other"},{"subitem_subject":"\u30de\u30a6\u30b9","subitem_subject_scheme":"Other"},{"subitem_subject":"\u88f8\u5316\u5375\u6bcd\u7d30\u80de","subitem_subject_scheme":"Other"},{"subitem_subject":"\u5375\u4e18\u3092\u6301\u3064\u5375\u6bcd\u7d30\u80de","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"\u8a00\u8a9e","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"\u8cc7\u6e90\u30bf\u30a4\u30d7","attribute_value_mlt":[{"resourcetype":"departmental bulletin 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Oocytes Cultured with and without Cumulus Cells and Possible Hyaluronan Production in Oocytes"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2021-03-01T11:01:59.936481+00:00"}