{"created":"2021-03-01T06:34:10.066683+00:00","id":28366,"links":{},"metadata":{"_buckets":{"deposit":"1542ba07-2118-493d-8ff5-de6ee2666e04"},"_deposit":{"id":"28366","owners":[],"pid":{"revision_id":0,"type":"depid","value":"28366"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00028366","sets":["453:456","485:872:1583:1597"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"サイトカラシンB 処置したマウス胚盤胞におけるハッチングとアクチンフィラメントの分布"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"79","bibliographicPageStart":"75","bibliographicVolumeNumber":"62","bibliographic_titles":[{"bibliographic_title":"新潟大学農学部研究報告"},{"bibliographic_title":"新潟大学農学部研究報告","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The hatching rate and the distribution of actin filaments were observed in mouse blastocysts whose ability of actin polymerization had been suppressed by cytochalasin B (CB), in order to examine the role of actin filaments in blastocyst hatching. The hatching rate of blastocysts developed from morulae in a medium containing 0.4μg/ml CB was 9.7%, which was significantly lower than the 78.1% of control blastocysts developed from morulae in a medium without CB. The fluorescence showing the presence of actin filaments was observed in the cytoplasm of trophectoderm and inner-cell-mass cells in blastocysts, and was especially strong in the peripheral cytoplasm where two trophectoderm cells adhering to each other. Also actin fluorescence was much brighter in protruded trophectoderm cells at the region of small hole or slit in the zona pellucida of hatching blastocysts. Such the distribution of actin filaments was similar in CB-treated and control blastocysts in each hatching period. On the other hand, the rate of CB-treated blastocysts in which most trophectoderm cells possess the fluorescence of actin filaments did not differ from that of control blastocysts in the post-hatching period, whereas such the rates of CB-treated blastocysts in the pre-hatching and hatching periods (34.1 and 46.5%) were significantly lower than the 95.0 and 82.9% of control blastocysts, respectively. The CB-treated blastocysts in the pre-hatching and hatching periods had many trophectoderm cells devoid of the fluorescence of actin filaments. From these findings, it was suggested that the polymerization of actin is essential for blastocyst hatching, and that actin filament-mediated movements of trophectoderm cells contribute to the hatching by facilitating the protrusion of trophectoderm cells from a small hole in the zona pellucida and also by enlarging the protrusion for slit formation in the zona pellucida.","subitem_description_type":"Abstract"},{"subitem_description":"サイトカラシンB（CB）で処置してアクチンの重合を抑制したマウス胚盤胞について、ハッチング能とアクチンフィラメントの分布を調べ、胚盤胞ハッチングに果たすアクチンフィラメントの役割を検討した。0.4μg/mlのCBを含む培養液で桑実胚から発生した胚盤胞のハッチング率は9.7％であり、対照のCBを含まない培養液で発生した胚盤胞の78.1％に比べ、有意に低かった。アクチンフィラメントの存在を示す特異蛍光は、栄養膜細胞と内細胞塊細胞の細胞質にみられ、栄養膜細胞同士が接する部位の細胞膜直下の細胞質で特に強かった。また、ハッチング中の胚盤胞において、この蛍光は透明帯の小孔から突出した栄養膜細胞および透明帯の裂け目付近の栄養膜細胞で特に強かった。このようなアクチンフィラメントの分布は、CB処置した胚盤胞と対照の胚盤胞の間で相違なかった。また、ほとんどの栄養膜細胞がアクチンフィラメントの存在を示す特異蛍光を有している胚盤胞の割合は、ハッチング後では、CB処置したものと対照のものとの間で相違なく、それぞれ75.0％と92.0％であった。一方、ハッチング前とハッチング中の期間において、ほとんどの栄養膜細胞がアクチンフィラメントの存在を示す特異蛍光を有しているCB処置胚盤胞の割合は、それぞれ34.1および46.5％であり、対照の胚盤胞の95.0および82.9％に比べて有意に低かった。以上のように、CBで処置してアクチンの重合を抑制したマウス胚盤胞では、ハッチング前とハッチング中にアクチンフィラメントを欠く栄養膜細胞が多数出現したために、このような胚盤胞ではハッチングの開始と完了に不可欠な透明帯での小孔形成と裂け目形成に必要な栄養膜細胞の運動が阻害され、結果としてハッチング能が低下したものと思われた。従って、アクチンフィラメントを介した栄養膜細胞の運動は、胚盤胞のハッチング過程の進行に重要な役割を果たしていることが考えられた。","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"163156","nameIdentifierScheme":"WEKO"}],"names":[{"name":"鈴木, 亮祐"}]},{"nameIdentifiers":[{"nameIdentifier":"163157","nameIdentifierScheme":"WEKO"}],"names":[{"name":"新村, 末雄"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟大学農学部"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00183393","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03858634","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Suzuki, Ryosuke"}],"nameIdentifiers":[{"nameIdentifier":"163154","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Niimura, Sueo"}],"nameIdentifiers":[{"nameIdentifier":"163155","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-20"}],"displaytype":"detail","filename":"62(2)_75-79.pdf","filesize":[{"value":"719.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"62(2)_75-79.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/28366/files/62(2)_75-79.pdf"},"version_id":"f5f19131-1586-4449-996d-a26ec1e7768d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"actin filament","subitem_subject_scheme":"Other"},{"subitem_subject":"blastocyst hatching","subitem_subject_scheme":"Other"},{"subitem_subject":"cytochalasin B","subitem_subject_scheme":"Other"},{"subitem_subject":"phallacidin","subitem_subject_scheme":"Other"},{"subitem_subject":"アクチンフィラメント","subitem_subject_scheme":"Other"},{"subitem_subject":"胚盤胞ハッチング","subitem_subject_scheme":"Other"},{"subitem_subject":"サイトカラシンB","subitem_subject_scheme":"Other"},{"subitem_subject":"ファラシジン","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Hatching and Distribution of Actin Filaments in Mouse Blastocysts Treated with Cytochalasin B","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Hatching and Distribution of Actin Filaments in Mouse Blastocysts Treated with Cytochalasin B"},{"subitem_title":"Hatching and Distribution of Actin Filaments in Mouse Blastocysts Treated with Cytochalasin B","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","1597"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-07-14"},"publish_date":"2010-07-14","publish_status":"0","recid":"28366","relation_version_is_last":true,"title":["Hatching and Distribution of Actin Filaments in Mouse Blastocysts Treated with Cytochalasin B"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:58:13.947232+00:00"}