{"_buckets": {"deposit": "ce1f9700-b1fe-42eb-9fa6-79e38d2ca4d3"}, "_deposit": {"id": "28363", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "28363"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00028363"}, "item_7_alternative_title_1": {"attribute_name": "\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "Synthesis of High Quality Probe Using Locked Nucleic Acid (LNA) and Exploitation for ISH"}]}, "item_7_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2010-08", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "39", "bibliographicPageStart": "35", "bibliographicVolumeNumber": "63", "bibliographic_titles": [{"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a"}, {"bibliographic_title": "\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8\u7814\u7a76\u5831\u544a", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "\u9ad8\u5ea6\u306b\u4fdd\u5b58\u3055\u308c\u305f\u5869\u57fa\u914d\u5217\u3092\u6709\u3059\u308bmRNA \u306ein situ hybridization\uff08ISH\uff09\u306b\u3088\u308b\u7279\u7570\u7684\u691c\u51fa\u3092\u76ee\u7684\u3068\u3057\u3066\u3001locked nucleicacid\uff08LNA\uff09\u3092\u5229\u7528\u3057\u305f\u30ad\u30e1\u30e9\u30aa\u30ea\u30b4\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u30d7\u30ed\u30fc\u30d6\u3092\u4f5c\u88fd\u3057\u3001\u305d\u306e\u5b9f\u7528\u6027\u3092\u8a55\u4fa1\u3057\u305f\u3002\u5869\u57fa\u914d\u5217\u306790\uff05\u4ee5\u4e0a\u306e\u76f8\u540c\u6027\u3092\u793a\u3059\u30ab\u30d6\u306e\u30cb\u30c8\u30ea\u30e9\u30fc\u30bc\u30a2\u30a4\u30bd\u30d5\u30a9\u30fc\u30e0BrNIT-T1\u53ca\u3073BrNIT-T2\u3068\u306e\u9593\u3067\u76f8\u540c\u6027\u306e\u4f4e\u304424\uff5e25\u5869\u57fa\u306e\u9818\u57df\u306b\u76f8\u88dc\u7684\u306a\u30aa\u30ea\u30b4\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u3092\u8a2d\u8a08\u3057\u3001\u4e21\u8005\u9593\u3067\u7570\u306a\u308b\u5869\u57fa\uff08\uff19\uff5e10\u5869\u57fa\uff09\u3092LNA \u306b\u7f6e\u63db\u3057\u305fLNA-DNA \u30ad\u30e1\u30e9\u30aa\u30ea\u30b4\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u3092\u4f5c\u88fd\u3057\u305f\u3002\u914d\u5217\u4e2d\u306bLNA \u3092\u90e8\u5206\u7684\u306b\u5c0e\u5165\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001DNA \u306e\u307f\u3067\u306f55\uff5e57\u2103\u3067\u3042\u3063\u305f\u30aa\u30ea\u30b4\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u306eTm \u5024\u306f78\uff5e82\u2103\u306b\u4e0a\u6607\u3057\u3001ISH \u89e3\u6790\u306b\u5341\u5206\u306a\u5024\u3092\u793a\u3057\u305f\u3002BrNIT-T1\u53ca\u3073BrNIT-T2\u306e\u5168\u9577\u8ee2\u5199\u7523\u7269\u306b\u5bfe\u3059\u308b\u7d50\u5408\u6027\u3092\u30c9\u30c3\u30c8\u30d6\u30ed\u30c3\u30c8\u30ce\u30b6\u30f3\u30cf\u30a4\u30d6\u30ea\u30c0\u30a4\u30bc\u30fc\u30b7\u30e7\u30f3\u306b\u3088\u308a\u8abf\u67fb\u3057\u305f\u3068\u3053\u308d\u3001BrNIT-T1\u53ca\u3073BrNIT-T2\u306e\u305d\u308c\u305e\u308c\u306e\u914d\u5217\u306b\u5bfe\u5fdc\u3059\u308bLNA-DNA \u30ad\u30e1\u30e9\u30d7\u30ed\u30fc\u30d6\u306f\u4e92\u3044\u306e\u8ee2\u5199\u7523\u7269\u306b\u975e\u7279\u7570\u7684\u306b\u7d50\u5408\u3059\u308b\u3053\u3068\u306a\u304f\u3001\u76ee\u7684\u306e\u8ee2\u5199\u7523\u7269\u306e\u307f\u3092\u691c\u51fa\u3067\u304d\u308b\u3053\u3068\u304c\u78ba\u8a8d\u3055\u308c\u305f\u3002\u3055\u3089\u306b\u3053\u308c\u3089\u306e\u30ad\u30e1\u30e9\u30d7\u30ed\u30fc\u30d6\u3092\u30ab\u30d6\u6839\u3053\u3076\u75c5\u7d44\u7e54\u306eISH \u89e3\u6790\u306b\u5fdc\u7528\u3057\u3001\u5f93\u6765\u306ecRNA \u30d7\u30ed\u30fc\u30d6\u3092\u7528\u3044\u305f\u89e3\u6790\u3067\u306f\u5f97\u3089\u308c\u306a\u304b\u3063\u305f\u3001BrNIT-T1\u3068BrNIT-T2\u306e\u6642\u7a7a\u9593\u7684\u306a\u767a\u73fe\u7279\u7570\u6027\u3092\u660e\u3089\u304b\u306b\u3059\u308b\u3053\u3068\u306b\u6210\u529f\u3057\u305f\u3002", "subitem_description_type": "Abstract"}, {"subitem_description": "To achieve specific detection of highly-conserved sequences by in situ hybridization (ISH), locked nucleic acid(LNA)-containing oligonucleotide probes were designed and assessed. Two isoforms of turnip nitrilases, BrNIT-T1 andBrNIT-T2, that possess more than 90% homology at the nucleotide level, were targeted in this study. Oligonucleotidescorresponding to a relatively different region between BrNIT-T1 and BrNIT-T2 were generated to contain LNAs in placeof DNA bases different between the two sequences. The partial incorporation of LNA into DNA oligonucleotides providedsufficient Tm values for ISH analysis. Dot-blot hybridization assay confirmed that the two LNA-modified oligonucleotideprobes corresponding to sequences of BrNIT-T1 and BrNIT-T2 specifically hybridized with the desired RNA sequencebut not with another one. Advantages of the LNA-modified probes in ISH analysis were also observed as specific andsensitive detection of BrNIT-T localization. Based on these results, we here report that partial incorporation of LNA intooligonucleotide probe is useful for highly specific detection of similar genes in ISH analysis.", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "163139", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Ishikawa, Toshiki"}]}, {"nameIdentifiers": [{"nameIdentifier": "163140", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Okazaki, Keiichi"}]}, {"nameIdentifiers": [{"nameIdentifier": "163141", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Itoh, Kimiko"}]}, {"nameIdentifiers": [{"nameIdentifier": "163142", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Mitsui, Toshiaki"}]}, {"nameIdentifiers": [{"nameIdentifier": "163143", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Hori, Hidetaka"}]}]}, "item_7_publisher_7": {"attribute_name": "\u51fa\u7248\u8005", 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"63(1)_35-39.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/28363/files/63(1)_35-39.pdf"}, "version_id": "e2fa8c1a-f76a-4583-82a4-6512194c663c"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "locked nucleic acid (LNA)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "in situ hybridization (ISH)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u30ad\u30e1\u30e9\u30aa\u30ea\u30b4\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u30d7\u30ed\u30fc\u30d6", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u30cb\u30c8\u30ea\u30e9\u30fc\u30bc", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Brassica rapa", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Chimera oligonucleotide probe", "subitem_subject_scheme": "Other"}, {"subitem_subject": "nitrilase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Brassica rapa", "subitem_subject_scheme": "Other"}]}, "item_language": 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