{"created":"2021-03-01T06:34:09.077966+00:00","id":28351,"links":{},"metadata":{"_buckets":{"deposit":"f52d2b3a-38a2-4707-8432-27482cce7a62"},"_deposit":{"id":"28351","owners":[],"pid":{"revision_id":0,"type":"depid","value":"28351"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00028351","sets":["453:456","485:872:1583:1595"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"モモアカアブラムシに殺虫活性を示すBacillus thuringiensisサイトトキシン、Cyt2Aa"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"81","bibliographicPageStart":"77","bibliographicVolumeNumber":"63","bibliographic_titles":[{"bibliographic_title":"新潟大学農学部研究報告"},{"bibliographic_title":"新潟大学農学部研究報告","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Aphid is a vector for various plant pathogenic viruses and gives severe damage various economically important cropsand vegetables. However, they are hardly controlled by biopesticides as they are living with phloem sap supplied by asucking the liquid. We investigated the aphidicidal activity of Bacillus thuringiensis toxin Cyt2Aa. The green peach aphid,Myzus persicae (Sulzer), were collected from field and reared with leaves of radish in a plastic column. In the bioassay, 100 μlof Cyt2Aa was given with sucrose solution at 200 μg/ml as final concentration through Parafilm and significant kill effectswas observed with 38% mortality at 72 h after the start of rearing. On the other hand, Cry1Aa, Cry1Ab and Cry1Ac as wellas PBS showed no activity. Admire, chemical pesticide (Bayer Cropsience, Monheim, Germany) which was used as positivecontrol kill all aphids tested. This is the first report showing aphidicidal activity of Cyt2Aa. Kill mechanism are also brieflydiscussed.","subitem_description_type":"Abstract"},{"subitem_description":"アブラムシは様々な植物病原ウイルスを媒介し、経済的に重要な作物や野菜に深刻な損害を与えている。持続的発展可能な農業の構築の為に生物農薬による防除体系の構築が求められるが、師管液を吸汁して生息するアブラムシに対して、生物農薬による有効な防除手段は報告されていない。そこで我々は、Bacillus thuringiensis が生産するCyt2Aa をアブラムシに吸汁させ、その殺虫活性について調査した。フィールドから採取したモモアカアブラムシ、Myzus persicae（Sulzer）をプラスチック製の柱状容器内で飼育し、Cyt2Aa の毒性試験を行なった。スクロース溶液と混合して最終濃度が200 μg/ml になるよう調整した100 μl のCyt2Aa を2重のパラフィルムで挟み、パラフィルム越しにアブラムシに吸汁させたところ、72時間で38% の有意な殺虫活性が示された。一方、Cry1Aa やCry1Ab、Cry1Ac は、ネガティブコントロールであるPBS 同様、アブラムシに対して殺虫活性を示さなかった。これに対し、化学農薬アドマイヤーは、用いた全アブラムシを殺虫した。アブラムシに吸汁された量を推論すると約0.2μg 以下のトキシンで殺虫されていると考えられた。本論文は、Cyt2Aa のアブラムシ殺虫活性を示した初めての報告であり、その殺虫メカニズムについても簡単に考察した。","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"163021","nameIdentifierScheme":"WEKO"}],"names":[{"name":"小山, 尚生"}]},{"nameIdentifiers":[{"nameIdentifier":"163022","nameIdentifierScheme":"WEKO"}],"names":[{"name":"佐野, 義孝"}]},{"nameIdentifiers":[{"nameIdentifier":"163023","nameIdentifierScheme":"WEKO"}],"names":[{"name":"田中, 未希"}]},{"nameIdentifiers":[{"nameIdentifier":"163024","nameIdentifierScheme":"WEKO"}],"names":[{"name":"萩野谷, 功輔"}]},{"nameIdentifiers":[{"nameIdentifier":"163025","nameIdentifierScheme":"WEKO"}],"names":[{"name":"プロモドンコイ, ブーヒアン"}]},{"nameIdentifiers":[{"nameIdentifier":"163026","nameIdentifierScheme":"WEKO"}],"names":[{"name":"アングスタナソンバット, チャナン"}]},{"nameIdentifiers":[{"nameIdentifier":"163027","nameIdentifierScheme":"WEKO"}],"names":[{"name":"三ツ井, 敏明"}]},{"nameIdentifiers":[{"nameIdentifier":"163028","nameIdentifierScheme":"WEKO"}],"names":[{"name":"高屋, 朋彰"}]},{"nameIdentifiers":[{"nameIdentifier":"163029","nameIdentifierScheme":"WEKO"}],"names":[{"name":"谷口, 正之"}]},{"nameIdentifiers":[{"nameIdentifier":"163030","nameIdentifierScheme":"WEKO"}],"names":[{"name":"岡崎, 桂一"}]},{"nameIdentifiers":[{"nameIdentifier":"163031","nameIdentifierScheme":"WEKO"}],"names":[{"name":"堀, 秀隆"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟大学農学部"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00183393","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03858634","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Koyama, Naoki"}],"nameIdentifiers":[{"nameIdentifier":"163010","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sano, Yoshitaka"}],"nameIdentifiers":[{"nameIdentifier":"163011","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tanaka, Miki"}],"nameIdentifiers":[{"nameIdentifier":"163012","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Haginoya, Kohsuke"}],"nameIdentifiers":[{"nameIdentifier":"163013","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Promodonkoy, Boonhiang"}],"nameIdentifiers":[{"nameIdentifier":"163014","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Angsuthanasombat, Chanan"}],"nameIdentifiers":[{"nameIdentifier":"163015","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mitsui, Toshiaki"}],"nameIdentifiers":[{"nameIdentifier":"163016","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Khoya, Tomoaki"}],"nameIdentifiers":[{"nameIdentifier":"163017","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tanigushi, Masayuki"}],"nameIdentifiers":[{"nameIdentifier":"163018","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okazaki, Keiichi"}],"nameIdentifiers":[{"nameIdentifier":"163019","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hori, Hidetaka"}],"nameIdentifiers":[{"nameIdentifier":"163020","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-20"}],"displaytype":"detail","filename":"63(2)_77-81.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"63(2)_77-81.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/28351/files/63(2)_77-81.pdf"},"version_id":"d3f5aca2-02bb-44f5-8378-f548f9c496fe"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Bacillus thuringiensis","subitem_subject_scheme":"Other"},{"subitem_subject":"Cyt2Aa","subitem_subject_scheme":"Other"},{"subitem_subject":"aphidicidal activity","subitem_subject_scheme":"Other"},{"subitem_subject":"Myzus persicae","subitem_subject_scheme":"Other"},{"subitem_subject":"Cry1Aa toxin","subitem_subject_scheme":"Other"},{"subitem_subject":"Cyt2Aa","subitem_subject_scheme":"Other"},{"subitem_subject":"アブラムシ殺虫活性","subitem_subject_scheme":"Other"},{"subitem_subject":"Cry1Aaトキシン","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Bacillus thuringiensis Cytotoxin, Cyt2Aa Killed Myzus persicae (Sulzer) (Hemiptera : Aphididae)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Bacillus thuringiensis Cytotoxin, Cyt2Aa Killed Myzus persicae (Sulzer) (Hemiptera : Aphididae)"},{"subitem_title":"Bacillus thuringiensis Cytotoxin, Cyt2Aa Killed Myzus persicae (Sulzer) (Hemiptera : Aphididae)","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","1595"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-07-02"},"publish_date":"2012-07-02","publish_status":"0","recid":"28351","relation_version_is_last":true,"title":["Bacillus thuringiensis Cytotoxin, Cyt2Aa Killed Myzus persicae (Sulzer) (Hemiptera : Aphididae)"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:58:21.209077+00:00"}