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While DSC measures the heat energy uptake that occurs in a protein molecule during thermal unfolding, ITCis used to measure the heat of binding of a protein to its ligand. The calorimetric approach enables accurate determinationof the thermodynamic parameters for thermal unfolding and binding reactions of proteins. In this manuscript, calorimetricanalysis of the effect of the disulfide bond on the structural stability and ligand binding capacity of the starch-binding domainof glucoamylase is described as an example.","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"\u8457\u8005\u5225\u540d","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"162959","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Sugimoto, Hayuki"}]}]},"item_7_publisher_7":{"attribute_name":"\u51fa\u7248\u8005","attribute_value_mlt":[{"subitem_publisher":"\u65b0\u6f5f\u5927\u5b66\u8fb2\u5b66\u90e8"}]},"item_7_select_19":{"attribute_name":"\u8457\u8005\u7248\u30d5\u30e9\u30b0","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00183393","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03858634","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"\u8457\u8005","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"\u6749\u672c, \u83ef\u5e78"}],"nameIdentifiers":[{"nameIdentifier":"162958","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"\u30d5\u30a1\u30a4\u30eb\u60c5\u5831","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-20"}],"displaytype":"detail","filename":"64(1)_87-92.pdf","filesize":[{"value":"919.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"64(1)_87-92.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/28346/files/64(1)_87-92.pdf"},"version_id":"8177c954-38d4-4661-a1f0-a8319af7e312"}]},"item_keyword":{"attribute_name":"\u30ad\u30fc\u30ef\u30fc\u30c9","attribute_value_mlt":[{"subitem_subject":"DSC","subitem_subject_scheme":"Other"},{"subitem_subject":"ITC","subitem_subject_scheme":"Other"},{"subitem_subject":"\u30bf\u30f3\u30d1\u30af\u8cea","subitem_subject_scheme":"Other"},{"subitem_subject":"\u71b1\u6e2c\u5b9a","subitem_subject_scheme":"Other"},{"subitem_subject":"\u71b1\u529b\u5b66","subitem_subject_scheme":"Other"},{"subitem_subject":"proteins","subitem_subject_scheme":"Other"},{"subitem_subject":"calorimetry","subitem_subject_scheme":"Other"},{"subitem_subject":"thermodynamics","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"\u8a00\u8a9e","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"\u8cc7\u6e90\u30bf\u30a4\u30d7","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"\u71b1\u6e2c\u5b9a\u6cd5\u306b\u3088\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u69cb\u9020\u3068\u6a5f\u80fd\u306e\u89e3\u6790","item_titles":{"attribute_name":"\u30bf\u30a4\u30c8\u30eb","attribute_value_mlt":[{"subitem_title":"\u71b1\u6e2c\u5b9a\u6cd5\u306b\u3088\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u69cb\u9020\u3068\u6a5f\u80fd\u306e\u89e3\u6790"},{"subitem_title":"\u71b1\u6e2c\u5b9a\u6cd5\u306b\u3088\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u69cb\u9020\u3068\u6a5f\u80fd\u306e\u89e3\u6790","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["453/456","485/872/1583/1594"],"pubdate":{"attribute_name":"\u516c\u958b\u65e5","attribute_value":"2012-07-02"},"publish_date":"2012-07-02","publish_status":"0","recid":"28346","relation_version_is_last":true,"title":["\u71b1\u6e2c\u5b9a\u6cd5\u306b\u3088\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u69cb\u9020\u3068\u6a5f\u80fd\u306e\u89e3\u6790"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2021-03-01T11:02:58.505785+00:00"}