@article{oai:niigata-u.repo.nii.ac.jp:00028344,
 author = {Nakai, Hiroyuki and Nihira, Takanori and Svensson, Birte and Otsubo, Kenichi},
 issue = {1},
 journal = {新潟大学農学部研究報告, 新潟大学農学部研究報告},
 month = {Sep},
 note = {A gene cluster involved in maltose/maltodextrin metabolism was identified in the genome of Lactobacillus acidophilusNCFM. Enzymatic properties was described for MalP belonging to glycoside hydrolase family 65 (GH65), which is encodedby malP (GenBank: AAV43670.1) located in the gene cluster. MalP catalyses phosphorolysis of maltose with inversion ofthe anomeric configuration releasing β-glucose 1-phosphate and glucose. Broad acceptor specificity was demonstrated byreverse phosphorolysis using various carbohydrate acceptors and β-glucose 1-phosphate as donor. MalP showed strongpreference for monosaccharide acceptors with equatorial 3-OH and 4-OH, and reacted also with the 2-deoxy amino sugars.By contrast none of the tested di- and trisaccharides served as acceptor. Disaccharide yields obtained from 50 mM β-glucose 1-phosphate and 50 mM glucose, glucosamine, N-acetyl glucosamine, mannose, xylose, or L-fucose were 99, 80, 53,93, 81, and 13%, respectively. Product structures were determined by NMR and ESI-MS to be α-D-glucopyranosyl-(1→4)-Dglucopyranose,α -D-glucopyranosyl-(1→4)-D-glucosaminopyranose,α -D-glucopyranosyl-(1→4)-N-acetyl-D-glucosaminopyranose,α -D-glucopyranosyl-(1→4)-D-mannopyranose, α-D-glucopyranosyl-(1→4)-D-xylopyranose, and α-D-glucopyranosyl-(1→4)-L-fucopyranose. Additionally MalP catalysed synthesis of the α-(1 → 4)-glucosidic disaccharides from maltose in a coupledphosphorolysis/reverse phosphorolysis one-pot reaction. Thus phosphorolysis of maltose to β-glucose 1-phosphatecircumvented addition of costly β-glucose 1-phosphate for reverse phosphorolysis with different monosacchaide acceptors.This strategy can be applied to large-scale production of valuable oligosaccharides from low-cost carbohydrates as catalysedby phosphorylases with different substrate specificity., Lactobacillus acidophilus NCFM のゲノム内に、マルトース/マルトデキストリン代謝に関与する遺伝子クラスターを見出した。本クラスター中には、グルコシドハイドロレースファミリー65に属する機能未知な糖質関連酵素（MalP）をコードする遺伝子が含まれている。そこで大腸菌を宿主として組み換え酵素を生産し、その酵素化学的諸性質を調査した結果、MalP はマルトースに特異的に作用する糖質加リン酸分解酵素であることが明らかになった。加えてMalP が触媒する糖質合成反応を用いて、６種のα（- 1→4）- グルコ二糖を高収率生産に成功した。},
 pages = {71--79},
 title = {Efficient Enzymatic Synthesis of α-(1→4)-glucosidic Disaccharides by Maltose Phosphorylase from Lactobacillus acidophilus NCFM},
 volume = {64},
 year = {2011}
}