@article{oai:niigata-u.repo.nii.ac.jp:00028336, author = {Sasaki, Taku and Kawanabe, Takahiro and Fujimoto, Ryo}, issue = {1}, journal = {新潟大学農学部研究報告, 新潟大学農学部研究報告}, month = {Sep}, note = {DNA methylation is an important epigenetic modification regulating gene expression. In this study, we investigateddifferences of DNA methylation status between leaves, stamens, and pistils by methylation sensitive amplificationpolymorphism (MSAP) analysis in Brassica rapa. The difference of DNA methylation status was the largest between leavesand stamens/pistils. About half of the differentially methylated sequences were genic regions, but their expression levelsdid not differ between organs. The percentage of genic regions detected by MSAP analysis was higher than that of the totallength of the genic regions in the genome, suggesting that genic regions are differentially methylated between organs in B.rapa., DNA のメチル化は遺伝子の転写制御に重要なエピジェネティックな変化である。本研究では、Brassica rapa の葉、雄しべ、雌しべのDNA のメチル化レベルの違いについてMSAP 法を用いて調べた。DNA のメチル化は葉と雄しべ/ 雌しべで最も違っていた。DNA のメチル化が異なっていた配列の半分は遺伝子領域であったが、遺伝子の発現量は3つの器官で違いが見られなかった。MSAP 法で見出された領域の内、遺伝子領域が占める割合は、ゲノム中に占める遺伝子領域の割合よりも高かったことから、B. rapa において、器官特異的なDNA のメチル化の違いは遺伝子領域に起こりやすい可能性が示された。}, pages = {7--16}, title = {Analysis of Organ-specific Regulation of DNA Methylation in Brassica rapa by MSAP}, volume = {64}, year = {2011} }