{"created":"2021-03-01T06:34:02.458354+00:00","id":28252,"links":{},"metadata":{"_buckets":{"deposit":"64ca6461-19cd-493d-b254-521e6d52f69b"},"_deposit":{"id":"28252","owners":[],"pid":{"revision_id":0,"type":"depid","value":"28252"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00028252","sets":["453:456","485:872:1583:1584"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"液胞型プロトン輸送性ピロフォスファターゼ（AVP1）遺伝子抑制変異体アラビドプシスの窒素集積に及ぼす硝酸およびアンモニア供給の効果"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-02","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"23","bibliographicPageStart":"15","bibliographicVolumeNumber":"70","bibliographic_titles":[{"bibliographic_title":"新潟大学農学部研究報告"},{"bibliographic_title":"新潟大学農学部研究報告","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"To investigate nitrogen accumulation in a transposon-silenced transgenic mutant of Arabidopsis vacuolar H^+-PPase (AVP1), ^<15>N-labeled nitrate and/or ammonium was supplied in the culture solution. When the mutant plants were supplied with ^<15>NO_3^-, ^<15>N accumulation in the shoots and roots was almost the same as in Columbia plants (Col). However, when the mutant plants were supplied with ^<15>NH_4^+, ^<15>N accumulated more highly in the cationic fraction in shoots compared with in Col. This increased accumulation was not detected when mutant plants were supplied with both ^<15>NO_3^- and ^<15>NH_4^+. Furthermore, it was observed that transposon-containing mutant plants could survive in low pH conditions (pH 2) for 12 days, suggesting that the pH in cell might be different from that in Col. These results suggested that the metabolism and utilization of nitrogen might be related to AVP1 in addition to PPi hydrolysis.","subitem_description_type":"Abstract"},{"subitem_description":"アラビドプシスのトランスポゾン挿入による液胞型H^+-PPase（AVP1）遺伝子抑制変異株を用い、水耕培地に与えた^<15>N で標識した硝酸態あるいはアンモニア態窒素の供給が窒素集積に及ぼす影響を調査した。遺伝子抑制変異株に^<15>N 標識硝酸を与えると、地上部および根における^<15>N の集積はコロンビアと同様であった。しかし、^<15>N 標識アンモニウムを遺伝子抑制変異株に与えると、^<15>N はコロンビアよりも地上部の陽イオン画分に多く集積した。この^<15>N 集積の増加は、遺伝子抑制変異株に^<15>N 標識硝酸と^<15>N 標識アンモニウム両方を供給した場合には認められなかった。さらに遺伝子抑制変異株は、低いpH 状態での12日間の培養でも生き残ることから、細胞内のpH がコロンビアと異なっていることが考えられた。これらの結果から、AVP1はピロリン酸の加水分解のみならず、窒素の利用や代謝にも関連することが示唆された。","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"162274","nameIdentifierScheme":"WEKO"}],"names":[{"name":"後藤, 大輝"}]},{"nameIdentifiers":[{"nameIdentifier":"162275","nameIdentifierScheme":"WEKO"}],"names":[{"name":"小野, 雄基"}]},{"nameIdentifiers":[{"nameIdentifier":"162276","nameIdentifierScheme":"WEKO"}],"names":[{"name":"伊藤, 紗織"}]},{"nameIdentifiers":[{"nameIdentifier":"162277","nameIdentifierScheme":"WEKO"}],"names":[{"name":"大竹, 憲邦"}]},{"nameIdentifiers":[{"nameIdentifier":"162278","nameIdentifierScheme":"WEKO"}],"names":[{"name":"末吉, 邦"}]},{"nameIdentifiers":[{"nameIdentifier":"52","nameIdentifierScheme":"WEKO"}],"names":[{"name":"大山, 卓爾"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟大学農学部"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00183393","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03858634","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Goto, Daiki"}],"nameIdentifiers":[{"nameIdentifier":"162268","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ono, Yuki"}],"nameIdentifiers":[{"nameIdentifier":"162269","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ito, Saori"}],"nameIdentifiers":[{"nameIdentifier":"162270","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ohtake, Norikuni"}],"nameIdentifiers":[{"nameIdentifier":"162271","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sueyoshi, Kuni"}],"nameIdentifiers":[{"nameIdentifier":"162272","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ohyama, Takuji"}],"nameIdentifiers":[{"nameIdentifier":"52","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-20"}],"displaytype":"detail","filename":"70_15-23.pdf","filesize":[{"value":"1.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"70_15-23.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/28252/files/70_15-23.pdf"},"version_id":"5c20380c-9d7e-40b3-9ff8-2df498f828f4"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"AVP1","subitem_subject_scheme":"Other"},{"subitem_subject":"silenced mutant","subitem_subject_scheme":"Other"},{"subitem_subject":"nitrate","subitem_subject_scheme":"Other"},{"subitem_subject":"ammonium","subitem_subject_scheme":"Other"},{"subitem_subject":"low pH","subitem_subject_scheme":"Other"},{"subitem_subject":"遺伝子抑制変異体","subitem_subject_scheme":"Other"},{"subitem_subject":"硝酸","subitem_subject_scheme":"Other"},{"subitem_subject":"アンモニア","subitem_subject_scheme":"Other"},{"subitem_subject":"低pH","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Effects of nitrate and ammonium application on nitrogen accumulation in an Arabidopsis gene silencing mutant of vacuolar pyrophosphatase1 (AVP1)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effects of nitrate and ammonium application on nitrogen accumulation in an Arabidopsis gene silencing mutant of vacuolar pyrophosphatase1 (AVP1)"}]},"item_type_id":"7","owner":"1","path":["456","1584"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-04-23"},"publish_date":"2018-04-23","publish_status":"0","recid":"28252","relation_version_is_last":true,"title":["Effects of nitrate and ammonium application on nitrogen accumulation in an Arabidopsis gene silencing mutant of vacuolar pyrophosphatase1 (AVP1)"],"weko_creator_id":"1","weko_shared_id":2},"updated":"2022-12-15T03:58:07.383402+00:00"}