{"created":"2021-03-01T06:06:32.727496+00:00","id":2814,"links":{},"metadata":{"_buckets":{"deposit":"eead19ce-08e1-4ce1-aaad-6bbd06dcf187"},"_deposit":{"id":"2814","owners":[],"pid":{"revision_id":0,"type":"depid","value":"2814"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00002814","sets":["453:454","485:486:487"]},"item_5_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"In vitro fertilization in the self-incompatible plant, Petunia hybrida"}]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1970-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"62","bibliographicPageStart":"56","bibliographicVolumeNumber":"39","bibliographic_titles":[{"bibliographic_title":"園芸学会雑誌"},{"bibliographic_title":"園芸学会雑誌","bibliographic_titleLang":"en"}]}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"自家不和合性ペチュニア（Petunia hybrida）と対照植物として自家不和合性ハナビシソウ（Eschscholtzia californica）を用いて試験管内受精により種子形成ができるかどうかを試みた．ハナビシソウの花粉は基本培地でよく発芽し花粉管伸長も十分であるが，ペチュニアの花粉はほとんど発芽しなかった．しかし花柱（柱頭付き）を使った花柱培養法やたいい座に着生したはい珠表面に花粉を直接散布した場合には基本培地での後者の花粉発芽，管伸長はある程度改善された．試験管内受精を行なった場合，ハナビシソウのはい珠は肥大するが発芽種子は得られなかった．その一部は脱分化を起しカルスを形成した．ペチュニアでは自家受粉（W166H），他家受粉（W166H×K146BH）にかかわらず子房あたり2，3個のはい珠が発達し，そのうちから健全な実生が得られた．","subitem_description_type":"Abstract"},{"subitem_description":"The possibility to obtain the seeds by the method of test-tube fertilization was examined using a self-incompatible plant, Petunia hybrida, and a self-compatible one, Eschscholtzia californica, as a control.\\r\\\\n 1. Pollen grains from both tested plants germinated and grew well on the sucrose-borate medium. On the basal medium (Table 1), pollens of E. californica germinated considerably, while those of P. hybrida hardly germinated. Pollen germination and tube growth of the latter were improved, however, in following two methods ; (a) “style culture” or (b) culture of whole mass of bare ovules with placental tissue dusted with pollen grains.\\r\\\\n 2. With E. californica, the ovules developed normally at the early period of the culture, but well developed seeds were not obtained in spite of the subculturing. Some of well swelled ovules formed callus having bud primordial like protuberances.\\r\\\\n With. P. hybrida ovules developed well by culturing the whole mass of bare ovules with pollen grains in both cross (fertile) or self (sterile) combinations. Some of the seedlings were obtained by subculturing the developed seeds onto basal medium in the former combination and also in the latter by employing MURASHIGE and SKOOG medium.","subitem_description_type":"Abstract"}]},"item_5_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"39190","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Niimi, Yoshiji"}]}]},"item_5_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"園芸学会"}]},"item_5_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"園芸学会"}]},"item_5_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_5_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00025257","subitem_source_identifier_type":"NCID"}]},"item_5_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00137626","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"新美, 芳二"}],"nameIdentifiers":[{"nameIdentifier":"39189","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-30"}],"displaytype":"detail","filename":"39(4)_56-62.pdf","filesize":[{"value":"4.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"39(4)_56-62.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/2814/files/39(4)_56-62.pdf"},"version_id":"d706132b-1a7b-4b05-a593-3aea194aa3c9"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ペチュニア（Petunia hybrida）の試験管内受精","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ペチュニア（Petunia hybrida）の試験管内受精"},{"subitem_title":"ペチュニア（Petunia hybrida）の試験管内受精","subitem_title_language":"en"}]},"item_type_id":"5","owner":"1","path":["454","487"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-10-21"},"publish_date":"2008-10-21","publish_status":"0","recid":"2814","relation_version_is_last":true,"title":["ペチュニア（Petunia hybrida）の試験管内受精"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:36:03.815924+00:00"}