@article{oai:niigata-u.repo.nii.ac.jp:00002813,
 author = {新美, 芳二},
 issue = {1},
 journal = {園芸学会雑誌, 園芸学会雑誌},
 month = {Mar},
 note = {In vitro culture of just fertilized ovules with placenta of P. hybrida, W166H×K146BH (each of clone is of self-incompatible), was conducted on some inorganic nutrient medium. Normal and abnormal seedlings developed from ovules with placenta at the stage of just fertilized ovules on MURASHIGE and SKOOG’s inorganic medium containing large quantities of KNO_3 and NH_4NO_3, but no seedlings on other inorganic media. To obtain many normal seedlings, effects of concentration of inorganic nitrogen KNO_3 and/or NH_4NO_3 on the growth of ovules were examined on NITSCH’s nutrient medium. The medium of NITSCH’s nutrient plus KNO_3 500 ppm and NH_4NO_3 80 ppm was better for growth of embryo than other media containing high nitrogen salts added to NITSCH’s nutrient medium. In conclusion, there were combined effects of NO_3-N and NH_4-N on the growth and development in vitro of P. hybrid embryo. Nitrogen salt solution for better growth was in a concentration of 12 mM and in ratio of NO_3-N to NH_4-N in molar unit of 11 : 1., 自家不和合性Petunia hybridaの2系統，W166HとK146BHの交雑受粉3日後すなわち受精直後の胎座つき胚珠の培養を行ない，無機塩培地を検討した。MURASHIGE&SKOOGの無機塩培地が他の培地ととくに違うところは窒素濃度が高いこと，ならびに硝酸態窒素のほかにアンモニア態窒素を含むことにあるので，この点について検討した。NITSCH培地を基本培地としてKNO_3とNH_4NO_3の濃度の組合わせ効果を調べたところKNO_3 500ppm，NH_4NO_3　80ppm添加した培地でもっとも多くの発芽種子が得られた。すなわち窒素塩として約12mM，そのうちアンモニウム塩として1mM，そしてNO_3-NとNH_4-Nの比率は11：1くらいが適当であろう。これらの窒素濃度が高い場合，胚発達は阻害され胚は奇形となった。},
 pages = {56--63},
 title = {Petunia hybridaの胚珠培養における窒素の濃度および形態が胚発達におよぼす影響},
 volume = {40},
 year = {1971}
}