{"created":"2021-03-01T06:06:32.598480+00:00","id":2812,"links":{},"metadata":{"_buckets":{"deposit":"b4decb86-0b19-40fd-b5c5-38004fc23e52"},"_deposit":{"id":"2812","owners":[],"pid":{"revision_id":0,"type":"depid","value":"2812"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00002812","sets":["453:454","485:486:487"]},"item_5_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Petunia hybridaのはい珠培養における鉄，ホウ酸，および糖が発芽種子数におよぼす影響"}]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1973-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"169","bibliographicPageStart":"163","bibliographicVolumeNumber":"42","bibliographic_titles":[{"bibliographic_title":"園芸学会雑誌"},{"bibliographic_title":"園芸学会雑誌","bibliographic_titleLang":"en"}]}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The present investigation deals with in vitro growth of ovules on a half of the placenta of petunia hybrid, W166H×K146BH (each clone is self-incompatible), with special reference to the influence of iron, boric acid, and sugars. The culture medium of NITSCH (1951), modified by the addition of 500,g/l KNO_3 and 80mg/l NH_4NO_3, was used as a basal medium. The optimal initial hydrogen-ion concentration was at pH of 4.5~5.0 in the basal medium and at pH of 6.0 in the basal medium with Fe-EDTA at an iron concentration of ca. 2.5 ppm instead of ferric citrate. The number of viable seeds per ovary increased with an increasing concentration of ferric citrate from 10 to 40 mg/l in the basal medium. Iron complex Fe-EDTA composed of 50mg/l FeSO_4・7H_2O and 40mg/l disodium ethylenediamineteraacetate, dehydrate (NA_2-EDTA) was also an effective compound and was favorable for the viable seed production. 10mg/l or above boric acid should be added to the basal medium containing either ferric citrate or Fe-EDTA. Medium containing 50mg/l boric acid was superior for the viable seed production to those with any other concentration. The efficancy of boron was influenced by the organic forms of iron. The medium with Fe-EDTA at an iron concentration of ca. 2.5 ppm was superior to that with 10mg/l ferric citrate. Basal medium containing 7.5 per cent sucrose or 5 per cent fructose produced the maximal viable seeds and that containing a mixture of sucrose and fructose or sucrose and glucose was inferior to the basal medium. From these results, experiments were conducted to clarify the interaction among three components, iron, boric acid, and sugars. About 5~6 normal seedlings with two cotyledons and roots per ovary were obtained in the medium containing ca. 10 ppm Fe as Fe-EDTA, 25mg/l boric acid, and 7.5 per cent sucrose. This was about six times as the number of seedlings produced in the basal medium. In the basal medium containing fructose instead of sucrose, however, the higher concentrations of boric acid and/or iron failed to bring about the noticeable effect.","subitem_description_type":"Abstract"},{"subitem_description":"自家不和合性Petunia hybridaの2系統，W116HとK146BHの交雑受粉3日後すなわち受精直後の胎座つきはい珠の培養を行ない，鉄，ホウ酸，および糖の種類ならびに濃度の発芽種子数におよぼす影響について検討した．基本培地が鉄源としてクエン酸第二化鉄を含む場合はpH 4.5～5.0，Fe-EDTAを含む場合は，pH 6.0付近が他のpH値よりもよかった．また鉄の濃度を従来より高めると発芽種子数が増加することもわかった．基本培地がクエン酸第二化鉄10mg/lまたはFe-EDTA（鉄として約2.5ppm）を含有した場合，ホウ酸の濃度を高めるとともに発芽種子数は増加し50mg/lが最適濃度であった．また鉄の種類が違うことによりホウ酸の効果に差が生じ，Fe-EDTAを含有する培地のほうがクエン酸第二化鉄を含む培地よりもその効果が大きかった．基本培地においてはい珠の発達に効果のあった糖はしょ糖と果糖であった．しょ糖7.5％と果糖5％を含有した培地間では実生数において差はなかったが，実生の大きさに関してしょ糖を含む培地から得られた実生は果糖を含む培地から得られたものより大きかった．以上の実験結果にもとづき，各要素の相互効果を検討したところ，Fe・EDTA（FeSO_4-7H_2O 50mg/l + Na_2-EDTA 40 mg/l），ホウ酸25mg/l，しょ糖7.5％を含む培地でもっとも多くの発芽種子がえられ，子房あたり約5から6本であった．それは基本培地から得られたものと比較し約6倍であった．また培地がしょ糖の代わりに果糖を含有する場合，ホウ素および鉄の濃度を高めても発芽種子数は増加しなかった．","subitem_description_type":"Abstract"}]},"item_5_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"39186","nameIdentifierScheme":"WEKO"}],"names":[{"name":"新美, 芳二"}]}]},"item_5_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"園芸学会"}]},"item_5_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"園芸学会"}]},"item_5_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_5_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00025257","subitem_source_identifier_type":"NCID"}]},"item_5_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00137626","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Niimi, Yoshiji"}],"nameIdentifiers":[{"nameIdentifier":"39185","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-30"}],"displaytype":"detail","filename":"42(2)_163-169.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"42(2)_163-169.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/2812/files/42(2)_163-169.pdf"},"version_id":"77f244a6-66fa-4667-8133-bc25de406ffe"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Influence of Iron, Boron, and Sugars on Viable Seed Production in Excised Ovule Culture in Petunia hybrida","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Influence of Iron, Boron, and Sugars on Viable Seed Production in Excised Ovule Culture in Petunia hybrida"},{"subitem_title":"Influence of Iron, Boron, and Sugars on Viable Seed Production in Excised Ovule Culture in Petunia hybrida","subitem_title_language":"en"}]},"item_type_id":"5","owner":"1","path":["454","487"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-10-21"},"publish_date":"2008-10-21","publish_status":"0","recid":"2812","relation_version_is_last":true,"title":["Influence of Iron, Boron, and Sugars on Viable Seed Production in Excised Ovule Culture in Petunia hybrida"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T04:34:22.982866+00:00"}