@article{oai:niigata-u.repo.nii.ac.jp:00002791, author = {新美, 芳二 and 権平, 正 and 轡田, 圭孝 and 辻, ひろ}, issue = {1}, journal = {園芸学会雑誌, 園芸学会雑誌}, month = {Jan}, note = {1.DIBA法のウイルス検出感度を調べるため,DIBA法とELISA法でユリ‘エンチャントメント’のりん片を供試し,ウイルス(LSV,TBV-L,CMV)の検出を行った.DIBA法の検出感度はELISA法の約1/10∿1/100であったが,ウイルスの検出は可能であることがわかった. 2.ユリのウイルスの検出に好適な試料を決めるため,花被,苞葉と茎の下部,中部および上部に着生した葉についてELISAの吸光度値(405nm)を比較した結果,上部着生葉で最も高い値が得られた.そして2品種および1種を供試し,上部および下部着生葉を開花約1カ月前から開花まで採集してELISA検定を行った結果,葉のELISAの吸光度値はいずれのユリでも開花期頃に最高となり,2品種では上部着生葉,1種では下部着生葉でそれぞれ高かった. 3.ほ場に生育中の18種類のユリの550個体の上部着生葉の基部切片を試料として,DIBA法で3種類のウイルスの検出を行った.調査個体の約73%からいづれかのウイルスが検出され,LSV,TBV-LおよびCMVが単独で検出された株はそれぞれ約52,2と1%で,残りの18%からは複数のウイルスが検出された.調査した18種類中のうち10種類のユリでは,80%以上の個体からウイルスが検出された. 4.LSVのみが検出されたユリではウイルスによる外部病徴はほとんど現れなかった.しかし,モザイク症状を呈した1種の葉からはLSVとTBV-Lが検出され,LSVとTBV-LあるいはCMVに重複感染した3品種の株は,茎の伸長がLSVのみ検出された株と比べ抑制された., 1. The viruses, LSV, TBV-L, and CMV were made in the scale-segments of Lilium X 'Enchantment' by both a dot immunobinding assay (DIBA) and indirect enzyme-linked immunosorbent assay (ELISA). The sensitivity of DIBA to detect viruses is about 10 to 100 times lower than that of ELISA, but it is an easy, reliable method to detect these viruses in routine surveys. 2. Absorbance values of LSV in leaves which were collected from the lower, middle, and upper parts of stem, perianths, and bracts were determined at 405 nm by ELISA. The most reliable value was obtained from the basal parts of leaves, located on the upper part of the stem. Based on this result, the absorbance of leaves collected at various times during the growth period was determined. The LSV level in the leaves increased gradually toward anthesis. Whereas the leaves excised from the upper part of the stem had a higher concentration of LSV than had those of the lower part in L. X 'Enchantment' and L. X 'Star Gazer', the reverse trend was found in L. formosanum. 3. Individual plants (550) of 18 Lilium spp. growing in the field were examined for three viruses by DIBA. Leaves of test plants were collected from the upper part of stems at anthesis and their basal parts were analyzed for three viruses. One or more viruses were detected in 73% of the 550 plants investigated. The frequency of LSV, TBV-L, and CMV occurring alone in the population was 52, 2, and 1%, respectively; the remaining 18% was infected with two or three viruses. More than 80% of the plants in 10 Lilium spp. were infected with one or more viruses. 4. Lilium spp. infected with LSV were nearly symptomless. Plants of L. rubellum with the leaves which develop green stripe mosaic were infected with LSV and TBV-L. Stem elongation of plants of 'Casablanca', 'Hinomoto', 'Enchantment', and 'Star Gazer' multiple-infected with LSV and TBV-L or CMV was retarded compared with stem elongation of the same cultivars infected with LSV alone.}, pages = {176--183}, title = {ほ場で栽培中のユリのLSV, TBV-LおよびCMVのELISA法とDIBA法による検出}, volume = {68}, year = {1999} }