{"created":"2021-03-01T06:06:30.955752+00:00","id":2786,"links":{},"metadata":{"_buckets":{"deposit":"d2503766-c9f8-4887-b42a-20280e3d8a01"},"_deposit":{"id":"2786","owners":[],"pid":{"revision_id":0,"type":"depid","value":"2786"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00002786","sets":["453:454","485:486:487"]},"author_link":["39045","39046","39047","39048"],"control_number":"2786","item_1627363077551":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_5_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"胚珠培養由来胚の子葉片培養法によるイヨウアジサイとアメリカノリノキとの種間雑種の作出","subitem_alternative_title_language":"ja"}]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1999-07","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"809","bibliographicPageStart":"803","bibliographicVolumeNumber":"68","bibliographic_titles":[{"bibliographic_title":"園芸学会雑誌","bibliographic_titleLang":"ja"}]}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A sexual incompatibility mechanism prevents reciprocal crosses between Hydrangea macrophylla f. hortensia (Lam.) Rehd. and H. arborescens L. No viable hybrid plants have been produced by conventional hybridization because the progenies exhibit hybrid lethality at a young seedling stage. For circumventing hybrid lethality, we cultured cotyledonary segments of embryos derived from crosses of H. macrophylla and H. arborescens. The cotyledonary segments formed callus. Fifteen callus lines, which regenerated plantlets, were established from crossings of H. macrophylla 'Blue sky'×H. arborescens 'Annabelle'. However, only a single callus line among them regenerated plantlets which could be transplanted to soil. Hybridity of the regenerated plants was examined by morphological, cytological characteristics, and RAPD markers: morphologically, the regenerated plants appeared intermediate to the parents; the regenerated plants were aneuploids having a chromosome number, 2n=42; whereas those of the parents H. macrophylla 'Blue sky' and H. arborescens 'Annabelle' were 2n=52 and 2n=38, respectively; the RAPD banding patterns of the regenerated plants exhibited the same hybrid bands as the parents. The hybridity of the regenerated plants was probably confirmed by the above methods.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"前報でセイヨウアジサイ[Hydrangea macrophylla f. hortensia (Lam.) Rehd.]とアメリカノリノキ(H. arborescens L.)の種間交雑において，交配後の胚珠培養で得られた実生が発芽直後あるいは幼苗期に枯死することを報告した．本研究では，雑種致死を回避し健全な雑種植物を得るため胚珠培養由来胚の子葉片を培養する方法を検討した．セイヨウアジサイ2品種を種子親，アメリカノリノキ1品種を花粉親にした種間交雑で胚珠培養により得た実生の子葉片を植物ホルモン添加培地で培養し，カルスを誘導した．‘ブルースカイ’を種子親にした場合，シュートを分化する15のカルス系統を選抜したが，鉢上げ後ガラス温室で順調に生育したのは1系統のみであった．再分化幼植物の雑種性を検定した結果，形態は両親のほぼ中間を示し，染色体数は，種子親の‘ブルースカイ’は2n=52本，花粉親の‘アナベル’は2n=38本であったのに対し，再分化植物のそれは2n=42本で異数性を示した．さらにRAPDのバンドパターンは両親のバンドを併せもった．以上から再分化植物の雑種性は高いと判断された．","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_5_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"39047","nameIdentifierScheme":"WEKO"}],"names":[{"name":"工藤, 暢宏","nameLang":"ja"}]},{"nameIdentifiers":[{"nameIdentifier":"39048","nameIdentifierScheme":"WEKO"}],"names":[{"name":"新美, 芳二","nameLang":"ja"}]}]},"item_5_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"園芸学会","subitem_publisher_language":"ja"}]},"item_5_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://doi.org/10.2503/jjshs.68.803","subitem_relation_type_select":"DOI"}}]},"item_5_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"園芸学会"}]},"item_5_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_5_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00025257","subitem_source_identifier_type":"NCID"}]},"item_5_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0013-7626","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kudo, Nobuhiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"39045","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Niimi, Yoshiji","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"39046","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-29"}],"displaytype":"detail","filename":"68(4)_803-809.pdf","filesize":[{"value":"1.9 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"68(4)_803-809.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/2786/files/68(4)_803-809.pdf"},"version_id":"d83f5384-c56b-4a35-bfb2-935d32bc5e00"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Hydrangea macrophylla","subitem_subject_scheme":"Other"},{"subitem_subject":"H. arborescens","subitem_subject_scheme":"Other"},{"subitem_subject":"cotyledonary segment culture","subitem_subject_scheme":"Other"},{"subitem_subject":"interspecific hybrid","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Production of Interspecific Hybrid Plants through Cotyledonary Segment Culture of Embryos Derived from Crosses between Hydrangea macrophylla f. hortensia (Lam.) Rehd. and H. arborescens L.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Production of Interspecific Hybrid Plants through Cotyledonary Segment Culture of Embryos Derived from Crosses between Hydrangea macrophylla f. hortensia (Lam.) Rehd. and H. arborescens L.","subitem_title_language":"en"}]},"item_type_id":"5","owner":"1","path":["454","487"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2008-10-17"},"publish_date":"2008-10-17","publish_status":"0","recid":"2786","relation_version_is_last":true,"title":["Production of Interspecific Hybrid Plants through Cotyledonary Segment Culture of Embryos Derived from Crosses between Hydrangea macrophylla f. hortensia (Lam.) Rehd. and H. arborescens L."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-15T04:33:12.862307+00:00"}