@article{oai:niigata-u.repo.nii.ac.jp:00002785,
 author = {Han, Dong-Sheng and Niimi, Yoshiji and Nakano, Masaru},
 issue = {5},
 journal = {園芸学会雑誌, Journal of the Japanese Society for Horticultural Science},
 month = {Sep},
 note = {An effective method for producing doubled haploid plants of the Asiatic hybrid lily 'Connecticut King' was established with an in vitro colchicine treatment of haploid calli. With an increase in the concentration and duration of colchicine treatment for the haploid calli, both the survival rate and the shoot regeneration from the calli decreased, but the frequency of diploid cells in the callus increased. Treatments of 0.25 and 0.5 mM colchicine for 48 or 72 hrs induced doubled haploid cells and shoot differentiation from calli. Haploid and diploid plantlets were regenerated from colchicine-treated calli, and only haploid plantlets were formed in colchicine-free treatments. This result suggests that these diploid plantlets originated from doubled haploid cells. Double haploids developed bulblets with more scaly leaves with longer stomatal guard cells than did the haploid plantlets., 本研究ではコルヒチン処理によるアジアティックハイブリッドユリ‘コネチカットキング’の倍加半数体植物の獲得方法について検討した．葯由来の半数性カルスの生存率とシュート再生率は，コルヒチン濃度の上昇および処理時間の延長にともなって低下したが，2倍性カルス細胞の割合は増加した．半数性カルス細胞の倍加とシュート再生率には，0.25mMと0.5mMのコルヒチンを用いた48時間または72時間処理が良好であることが明らかとなった．コルヒチン処理された半数性カルスは半数体と2倍体植物を再生したが，対照区(コルヒチン無処理)のカルスは半数性植物のみを再生したことから，これらの2倍体植物は半数性細胞が倍加されたことにより得られた倍加半数体植物であることが推察された．倍加半数体植物では，葉の気孔サイズは半数体植物のものと比べ大きく，子球もよく発達した．},
 pages = {979--983},
 title = {Production of Doubled Haploid Plants Through Colchicine Treatment of Anther-derived Haploid Calli in the Asiatic Hybrid Lily ‘Connecticut King’},
 volume = {68},
 year = {1999}
}