@article{oai:niigata-u.repo.nii.ac.jp:00002783,
 author = {Han, Dong-Sheng and Niimi, Yoshiji and Nakano, Masaru},
 issue = {1},
 journal = {園芸学会雑誌},
 month = {Jan},
 note = {ユリにおいて単離小胞子からのカルスの誘導に初めて成功した．圃場で栽培した‘コネチカットキング’から花らい（長さ2-3cm）を採取し，やくから1核期の小胞子を単離し，25℃，暗黒下で培養した．小胞子の生存と発達に及ぼす炭素源（ショ糖およびマルトース）と培地更新の影響をMS培地を用いて検討した．培地の炭素源は培養小胞子の生存率および発達に影響した．小胞子の生存率はショ糖よりもマルトースを添加した培地で高かった．培地の更新は小胞子の生存率を改善しなかった．ショ糖培地では，多数の小胞子がデンプン粒を蓄積し，それは培養を継続しても分裂しなかった．一方，マルトース培地では，デンプン粒を含む，膨張した小胞子はほとんどなく，小胞子の分裂が観察された．これらの小胞子は分裂を繰り返し，多細胞小胞子やカルスに発達した．, Calli from microspores were induced for the first time in Lilium species. The uninucleate microspores of the Asiatic hybrid lily 'Connecticut King' were isolated and cultured in liquid medium, containing half strength of MS macronutrients, full strength of micronutrients, Fe-EDTA and vitamins of MS, 100mg・liter^-1 glutamine, 1mg・liter^-1 picloram and 0.25 M sucrose or maltose. Microspore viability and development of cultured microspores were influenced by the carbohydrate sources in cultre medium. A relatively high viability rate of microspores was observed in the maltose medium, compared with that of sucrose. Cell divisions of microspores and callus formation were found in the maltose medium, whereas in the sucrose medium, a large number of swollen microspores containing many starch grains remained undivided. Hence, maltose is the preferred carbohydrate source for microspore culture of 'Connecticut King'.},
 pages = {52--56},
 title = {Formation of Calli from Isolated Microspore Cultures of Asiatic Hybrid Lily ‘Connecticut King’},
 volume = {69},
 year = {2000}
}