@article{oai:niigata-u.repo.nii.ac.jp:00002616,
 author = {Nozawa-Inoue, Kayoko and Amizuka, Norio and Suzuki, Akiko and Maeda, Takeyasu},
 issue = {2},
 journal = {The Anatomical Record Part A: Discoveries in Molecular, Cellular, and Evolutionary Biology, The Anatomical Record Part A: Discoveries in Molecular, Cellular, and Evolutionary Biology},
 month = {Jun},
 note = {One series of our research has shown an intense expression of immunoreaction for heat shock protein 25 (Hsp25) in various cellular elements in the rat temporomandibular joint (TMJ). This protein is the major substrate of mitogen-activated protein kinase-activated protein kinase-2 (MAPKAPK-2), which mediates an intracellular stress-activated signaling pathway to stimulate cytosolic actin reorganization under various stresses. The present study was undertaken to examine the localization of MAPKAPK-2 in the rat TMJ by immunocytochemical techniques. Furthermore, confocal microscopy with double staining was employed to demonstrate the co-localization of MAPKAPK-2 and Hsp25. Immunocytochemistry for MAPKAPK-2 showed an intense immunoreaction in the cytoplasm of the synovial lining cells, the endothelial cells, and the fibroblasts in the synovial membrane of the rat TMJ. Double immunostaining under a confocal microscope succeeded in demonstrating the co-localization of MAPKAPK-2- and Hsp25-immunoreactions in the cytoplasm of fibroblastic type B synoviocytes in the TMJ. On the other hand, the macrophage-like type A cells expressed MAPKAPK-2-immunoreactions but lacked Hsp25-immunoreactivity. The cells in the articular disc and the chondrocytes in the maturative and hypertrophic layer of the mandibular cartilage also showed intense immunoreactions for MAPKAPK-2 and Hsp25. In addition to cytoplasmic localization, MAPKAPK-2- immunoreactions were found in the nucleus of some synovial lining cells, cells in the articular disc, and chondrocytes. Current observations imply that the presence of the phosphorylation of Hsp25 via activated MAPKAPK-2 in the cytoplasm. The MAPKAPK-2 and Hsp25 possibly participate in the induction of cytoskeletal changes to the various cellular elements in rat TMJ under normal conditions.},
 pages = {522--528},
 title = {Immunocytochemical Localization of MAPKAPK-2 and Hsp25 in the Rat Temporomandibular Joint},
 volume = {284A},
 year = {2005}
}