{"created":"2021-03-01T06:31:41.121210+00:00","id":26134,"links":{},"metadata":{"_buckets":{"deposit":"46a7ab32-41a4-4c2a-9d66-c13edeca0f2d"},"_deposit":{"id":"26134","owners":[],"pid":{"revision_id":0,"type":"depid","value":"26134"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00026134","sets":["432:1291:1292:1344","453:456"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Studies on the osteogenicity of bone-forming cells derived from newborn mouse calvaria"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1987-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"6","bibliographicPageStart":"1","bibliographicVolumeNumber":"17","bibliographic_titles":[{"bibliographic_title":"新潟歯学会雑誌"},{"bibliographic_title":"新潟歯学会雑誌","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"我々は,マウス新生仔頭蓋骨より分離した骨細胞をアルカリホスファターゼ(ALP)活性を維持した状態で6カ月以上35代まで継代培養できたことについて報告する｡造骨細胞は, マウス新生仔頭蓋骨よりcoll-genase / dispase II 処理によって分離し,α-MEM+15%FCSで5%CO_2, 37℃ 条件下で培養した｡継代数3-6代で培加時間が最も長くなったが,それ以降は増殖率も高まると同時に形態的にも安定した｡この細胞のALP活性は,コンフルエントシート形成後,経時的に上昇する傾向を持ち,それに伴い石灰化物の形成が観察された｡また,insulin, prostaglandin E_2(PGE_2),血清に応答してALP活性の上昇が観察された｡以上の結果から,我々の維持している細胞は骨芽細胞様であることが示唆された｡","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"154356","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Kawase, Tomoyuki"}]},{"nameIdentifiers":[{"nameIdentifier":"154357","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Ishikawa, Ichijiro"}]},{"nameIdentifiers":[{"nameIdentifier":"154358","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Suzuki, Akitoshi"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟歯学会"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN0018300X","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03850153","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"川瀬, 知之"}],"nameIdentifiers":[{"nameIdentifier":"154353","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"石川, 市次郎"}],"nameIdentifiers":[{"nameIdentifier":"154354","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鈴木, 暲俊"}],"nameIdentifiers":[{"nameIdentifier":"154355","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-19"}],"displaytype":"detail","filename":"NS_17(1)_1-6.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"NS_17(1)_1-6.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/26134/files/NS_17(1)_1-6.pdf"},"version_id":"897e26d0-b6f0-4ad6-bd68-5d085b4d0f22"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Bone-forming cells","subitem_subject_scheme":"Other"},{"subitem_subject":"Serial digestion","subitem_subject_scheme":"Other"},{"subitem_subject":"Alkaline phosphatase","subitem_subject_scheme":"Other"},{"subitem_subject":"Nodule","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"マウス新生仔頭蓋骨より分離･培養した造骨細胞の石灰化能に関する研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"マウス新生仔頭蓋骨より分離･培養した造骨細胞の石灰化能に関する研究"},{"subitem_title":"マウス新生仔頭蓋骨より分離･培養した造骨細胞の石灰化能に関する研究","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","1344"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-07-13"},"publish_date":"2012-07-13","publish_status":"0","recid":"26134","relation_version_is_last":true,"title":["マウス新生仔頭蓋骨より分離･培養した造骨細胞の石灰化能に関する研究"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:56:10.587922+00:00"}