{"created":"2021-03-01T06:31:35.317234+00:00","id":26047,"links":{},"metadata":{"_buckets":{"deposit":"4d4b64c9-83a6-4c0e-a62f-8ec955eb6ed1"},"_deposit":{"id":"26047","owners":[],"pid":{"revision_id":0,"type":"depid","value":"26047"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00026047","sets":["432:1291:1292:1335","453:456"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Localization of Interleukin-1 (IL-1) mRNA-expressing macrophages in human inflamed gingiva and IL-1 activity in gingival crevicular fluid"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1991-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"64","bibliographicPageStart":"53","bibliographicVolumeNumber":"21","bibliographic_titles":[{"bibliographic_title":"新潟歯学会雑誌"},{"bibliographic_title":"新潟歯学会雑誌","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The purpose of this study was to determine the exact cell type and site(s) involved in interleukin-1 (IL-1) production during gingival inflammation. By combining immunohistochemistry and in situ hybridization, IL-1messenger RNA (mRNA) –expressing cells in human inflamed gingival were identified as macrophages. The rate of IL-1α mRNA expression in these macrophages was the same as IL-1βmRNA expression. Additionally, the rate of IL-1 mRNA expression was higher in tissue sections furthest from sulcular areas than in those closest of sulcular lesions, although more macrophages were present at the sulcular site. The IL-1 activity in gingival crevicular fluid (GCF) obtained from inframed gingival was higher than that from healthy gingival and decreased after periodontal therapy. The IL-1 activity in GCF was almost completely abolished by the addition of anti-IL-1α antibody but not anti-IL-1βantibody, indicating that IL-1α is the predominant form in GCF. However, the IL-1 activity in GCF was unrelated to the number of IL-1 mRNA-expressing macrophages in the same gingival site where the GCF was obtained at the same time. The results suggest that macrophages in the oral side contribute to the production of IL-1 but those in the sulcular side play a different role in the generation of gingival inflammation.","subitem_description_type":"Abstract"},{"subitem_description":"歯周炎罹患者の歯肉組織中におけるインターリュウキン1 (IL-1) mRNA発現細胞の局在及び歯肉溝滲出液 (GCF) 中のIL-1活性について検索した｡歯周炎罹患者28名のうち19名から歯周外科時に歯肉組織を採取し､18名からGCFを採取した｡対照群としての歯肉組織は､本学矯正科に来院した患者から歯の便宜抜去時に採取し､GCFは歯肉健常者のボランティアから採取した｡採取後の歯肉組織はパラフィン包埋切片としPAP法による免疫組織化学的にマクロファージ､T細胞､B細胞､内皮細胞を染色し､同一切片上でin situ hybridizationをIL-1α､IL-1βについて^35S標識のプローブを用いて行った｡GCFのIL-1活性はC3H/HeJマウスの胸腺細胞の増殖を^3H-thymidinの取り込み量で測定した｡また､α/βをみるために中和実験を行った｡その結果､歯周炎罹患歯肉組織中のIL-1mRNA発現マクロファージはポケット側より口腔側に多く認められ､その局在に関しては､炎症局所における機能的対応が示唆された｡GCF中のIL-1活性は健常者に比べ歯周炎罹患者で有意に高く､歯周治療により有意に減少した｡また､α タイプが圧倒的に多くその活性を占めていた｡GCF中のIL-1活性は､歯周炎の病態変化と深い関係が認められることから､新しい臨床診断法としての可能性が期待される。","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"153852","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Matsuki, Yutaka"}]},{"nameIdentifiers":[{"nameIdentifier":"153853","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Yamamoto, Tadashi"}]},{"nameIdentifiers":[{"nameIdentifier":"153854","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Hara, Kohji"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟歯学会"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN0018300X","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03850153","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"松木, 裕"}],"nameIdentifiers":[{"nameIdentifier":"153849","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山本, 格"}],"nameIdentifiers":[{"nameIdentifier":"153850","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"原, 耕二"}],"nameIdentifiers":[{"nameIdentifier":"153851","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-19"}],"displaytype":"detail","filename":"NS_21(2)_53-64.pdf","filesize":[{"value":"2.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"NS_21(2)_53-64.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/26047/files/NS_21(2)_53-64.pdf"},"version_id":"13c98919-4f1c-4fd4-a27d-8c0839658687"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"インターリュウキソ1","subitem_subject_scheme":"Other"},{"subitem_subject":"マクロファージ","subitem_subject_scheme":"Other"},{"subitem_subject":"in situ hybridization","subitem_subject_scheme":"Other"},{"subitem_subject":"歯肉溝滲出液","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"歯周炎罹患歯肉組織中のインターリュウキン1(IL-1)mRNA発現マクロファージの局在と歯肉溝滲出液中IL-1活性","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"歯周炎罹患歯肉組織中のインターリュウキン1(IL-1)mRNA発現マクロファージの局在と歯肉溝滲出液中IL-1活性"},{"subitem_title":"歯周炎罹患歯肉組織中のインターリュウキン1(IL-1)mRNA発現マクロファージの局在と歯肉溝滲出液中IL-1活性","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","1335"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-07-29"},"publish_date":"2012-07-29","publish_status":"0","recid":"26047","relation_version_is_last":true,"title":["歯周炎罹患歯肉組織中のインターリュウキン1(IL-1)mRNA発現マクロファージの局在と歯肉溝滲出液中IL-1活性"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:56:06.826013+00:00"}