{"_buckets": {"deposit": "6713775c-afc9-40f7-a0dd-69cdf53d654b"}, "_deposit": {"id": "26020", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "26020"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00026020"}, "item_7_alternative_title_1": {"attribute_name": "\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "Expression of TIMP-1, TIMP-2 and collagenase mRNA in periodontitis-affected human gingival tissue"}]}, "item_7_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1992-12", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "13", "bibliographicPageStart": "1", "bibliographicVolumeNumber": "22", "bibliographic_titles": [{"bibliographic_title": "\u65b0\u6f5f\u6b6f\u5b66\u4f1a\u96d1\u8a8c"}, {"bibliographic_title": "\u65b0\u6f5f\u6b6f\u5b66\u4f1a\u96d1\u8a8c", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Collagenolysis in periodontitis is thought to be modulated by the expression of three genes, one for collagenase and the two others for tissue inhibitors of metalloproteinases-1 and-2(TIMP-1and-2). We assessed possible difference in TIMP-1, TIMP-2 and collagenase mRNA levels between gingival samples from patients with periodontitis and those from healthy subjects by reverse transcription-polymerase chain reaction (RT-PCR). The technique allows detection of transcripts from a very small sample quantity. The experiments showed levels of TIMP-1 and collagenase transcripts relative to \u03b2-actin to be significantly higher in the diseased group than in healthy controls (8.11\u00b10.83 versus 1.38\u00b10.28% for TIMP-1 and 0.50\u00b10.10 versus 0.0075\u00b10.0024% for collagenase, respectively). The difference in TIMP-2 was not significant between the two groups (2.91\u00b10.46 versus 1.84\u00b10.87%). Our results suggest that the host responded to the increase in collagenase level by preferentially producing TIMP-1 to overcome tissue destruction due to periodontitis. The differential gene expression of TMP-1 and TIMP-2 in our study may be accounting for a distinct genetic regulation of TIMP-1 and -2 in vivo.", "subitem_description_type": "Abstract"}, {"subitem_description": "\u6b6f\u5468\u708e\u7f79\u60a3\u6b6f\u8089\u53ca\u3073\u5065\u5e38\u6b6f\u8089\u306b\u304a\u3051\u308bcollagenase, TIMP-1,-2\u306emRNA\u767a\u73fe\u3092\u691c\u7d22\u3059\u308b\u305f\u3081\u306b\uff64\u9006\u8ee2\u5199PCR(reverse transcription-polymerase chain reaction: RT-PCR)\u6cd5\u3068\u3044\u3046\u6975\u3081\u3066\u9ad8\u611f\u5ea6\u306emRNA\u691c\u7d22\u6cd5\u3092\u7528\u3044\u3066\uff64\u6b6f\u5468\u708e\u60a3\u800510\u540d\u53ca\u3073\u5065\u5e38\u80055\u540d\u3088\u308a\u5f97\u305f\u5fae\u91cf\u6b6f\u8089\u30b5\u30f3\u30d7\u30eb\u4e2d\u306e\u5404mRNA\u767a\u73fe\u30ec\u30d9\u30eb\u3092\u540c\u6642\u306b\u691c\u7d22\u3057\u305f\uff61mRNA\u306e\u691c\u51fa\u306b\u969b\u3057\u3066\u306f\uff64\u03b2-actin (house.-keeping\u907a\u4f1d\u5b50\u306e\u3072\u3068\u3064\u3067\u3042\u308a\u305d\u306e\u767a\u73fe\u304c\u6bd4\u8f03\u7684\u4e00\u5b9a\u3067\u3042\u308b)\u3092\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3068\u3057\u3066\u7528\u3044\uff64\u5404mRNA\u306e\u767a\u73fe\u30ec\u30d9\u30eb\u3092\u03b2-actin\u306emRNA \u30ec\u30d9\u30eb\u306b\u5bfe\u3059\u308b\u6bd4\u7387%\u3068\u3057\u3066\u6c42\u3081\u305f\uff61\u305d\u306e\u7d50\u679c\uff64\u6b6f\u5468\u708e\u60a3\u8005\u6b6f\u8089\u306b\u304a\u3051\u308bTIMP-1\u3068collagenase\u306emRNA \u30ec\u30d9\u30eb\u306f\uff64\u5065\u5e38\u8005\u6b6f\u8089\u306b\u6bd4\u3079\u6709\u610f\u306b\u9ad8\u3044\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u306a\u3063\u305f(mean\u00b1SE ; TIMP-1 : 8.11\u00b10.83vs1.38\u00b10.28%, collagenase : 0.50\u00b10.10vs0.0075\u00b10.0024%)\u3002\u305d\u308c\u306b\u5bfe\u3057\uff64TIMP-2\u306emRNA \u30ec\u30d9\u30eb\u306b\u3064\u3044\u3066\u306f\u60a3\u8005\u3068\u5065\u5e38\u8005\u3067\u6709\u610f\u5dee\u306f\u5f97\u3089\u308c\u306a\u304b\u3063\u305f(2.91\u00b10.46vs1.84\u00b10.87%)\u3002\u3053\u308c\u3089\u306e\u7d50\u679c\u304b\u3089\uff64\u708e\u75c7\u6b6f\u8089\u306b\u304a\u3044\u3066\u306f\uff64collagenase \u7523\u751f\u306e\u4e0a\u6607\u306b\u3088\u308b\u7d50\u5408\u7d44\u7e54\u7834\u58ca\u304c\u751f\u3058\u3066\u3044\u308b\u3068\u540c\u6642\u306b\uff64\u7834\u58ca\u306e\u9032\u884c\u3092\u6291\u3048\u3088\u3046\u3068\u3059\u308b\u751f\u4f53\u306e\u9632\u5fa1\u53cd\u5fdc\u3068\u3057\u3066TIMP-1\u7523\u751f\u306e\u4e0a\u6607\u304c\u8d77\u3053\u3063\u3066\u3044\u308b\u3053\u3068\uff64\u307e\u305f\uff64TIMP-1\u3068TIMP-2\u306f\u7570\u306a\u308b\u907a\u4f1d\u5b50\u8abf\u7bc0\u3092\u53d7\u3051\u3066\u304a\u308a\uff64\u7d44\u7e54\u7834\u58ca\u306b\u5bfe\u3059\u308b\u9632\u5fa1\u6a5f\u69cb\u3068\u3057\u3066\u306fTIMP-2\u3088\u308a\u3082TIMP-1\u306e\u65b9\u304c\u9078\u629e\u7684\u306b\u52d5\u54e1\u3055\u308c\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b\u3053\u3068\u304c\u793a\u5506\u3055\u308c\u305f\u3002", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "153627", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Nomura, Takashi"}]}, {"nameIdentifiers": [{"nameIdentifier": "153628", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Takaahshi, Tokuya"}]}, {"nameIdentifiers": [{"nameIdentifier": "153629", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Hara, Kohji"}]}]}, "item_7_publisher_7": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "\u65b0\u6f5f\u6b6f\u5b66\u4f1a"}]}, "item_7_select_19": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AN0018300X", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "03850153", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u91ce\u6751, \u9686"}], "nameIdentifiers": [{"nameIdentifier": "153624", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "\u9ad8\u6a4b, \u5fb3\u4e5f"}], "nameIdentifiers": [{"nameIdentifier": "153625", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "\u539f, \u8015\u4e8c"}], "nameIdentifiers": [{"nameIdentifier": "153626", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-19"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "NS_22(2)_1-13.pdf", "filesize": [{"value": "2.2 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 2200000.0, "url": {"label": "NS_22(2)_1-13.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/26020/files/NS_22(2)_1-13.pdf"}, "version_id": "65f260d0-4476-4d81-bc94-7754f504f026"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "TIMP-gene", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Collagenase-gene", "subitem_subject_scheme": "Other"}, {"subitem_subject": "RT-PCR", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Periodontitis", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "\u30d2\u30c8\u6b6f\u5468\u708e\u7f79\u60a3\u6b6f\u8089\u306b\u304a\u3051\u308bTIMP-1, TIMP-2 \u304a\u3088\u3073Collagenase mRNA\u767a\u73fe\u306e\u691c\u7d22", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "\u30d2\u30c8\u6b6f\u5468\u708e\u7f79\u60a3\u6b6f\u8089\u306b\u304a\u3051\u308bTIMP-1, TIMP-2 \u304a\u3088\u3073Collagenase mRNA\u767a\u73fe\u306e\u691c\u7d22"}, {"subitem_title": "\u30d2\u30c8\u6b6f\u5468\u708e\u7f79\u60a3\u6b6f\u8089\u306b\u304a\u3051\u308bTIMP-1, TIMP-2 \u304a\u3088\u3073Collagenase mRNA\u767a\u73fe\u306e\u691c\u7d22", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["453/456", "432/1291/1292/1333"], "permalink_uri": "http://hdl.handle.net/10191/19213", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2012-07-30"}, "publish_date": "2012-07-30", "publish_status": "0", "recid": "26020", "relation": {}, "relation_version_is_last": true, "title": ["\u30d2\u30c8\u6b6f\u5468\u708e\u7f79\u60a3\u6b6f\u8089\u306b\u304a\u3051\u308bTIMP-1, TIMP-2 \u304a\u3088\u3073Collagenase mRNA\u767a\u73fe\u306e\u691c\u7d22"], "weko_shared_id": null}