@article{oai:niigata-u.repo.nii.ac.jp:00025889, author = {鶴巻, 浩 and 大橋, 靖 and 鈴木, 誠}, issue = {2}, journal = {新潟歯学会雑誌, 新潟歯学会雑誌}, month = {Dec}, note = {The purpose of this study was to identify sequential histological alterations and changes in the vascular system in normal tongue of golden hamsters followlng hyperthermia treatment. Adult golden hamsters of both sexes were divided into 2 heated groups and a control group. The tongue was heated with a 13.56MHz radiofrequency (RF) capacitive heating system. In the 2 heated groups, the inside of the tongue was heated to 42.0℃ and 43.0℃, respectively, for 40 minutes. Histological changes were investigated by light microscopy, and vascular changes were observed by scanning electron microscopy of vascular casts. The results were as follows; 1. Histologically, in the 42℃ treated group, changes such as relatively mild edema, capillary dilatation, and congestion were observed, but there was almost complete recovery within 48 hours. In the 43℃ treated group, however, severe congestion, interstitial edema, focal hemorrhage and capillary rupture occurred immediately after heating, and interstitial fibrosis was present 4 weeks after heating. 2. Scanning electron microscopic examination in the 42℃ treated group showed avascular areas where the resin was not present in part of the tongue surface within 24 hours, but this was no longer observed 48 hours after heating. In the 43℃ treated group, there were very extensive avascular areas in the heated area from immediately after heating up to 48 hours later, and morphologic irregularities in the vascular network were observed 1 week after heating. 3.These results indicate that transient vascular injury may occur as a result of 42℃ hyperthermia, but that severe circulatory impairment and irreversible vascular injury occur when normal vessels are exposed to 43℃ hyperthermia. 4. This tongue model was very useful for observing vascular injury induced by hyperthermia., 本研究の目的は,正常舌組織をモデルとして,加温による血管障害の経時的変化を明らかにすることである。成熟ゴールデン-ムスターの正常舌を加温対象とし,加温装置は周波数13.56MHzのRF誘電型加温システムを使用した。設定温度は42.0℃および43.0℃とし,各々40分間1回加温を行った。その経時的変化について,組織学的観察および血管鋳型法を用いた電顕的観察を行い,以下の結論を得た。1.組織学的観察においては,42℃加温群では,血管拡張,うっ血,問質浮腫等の変化がみられたものの,ほぼ4間で回復した。43℃加温群では,強度のうっ血,問質浮腫,出血および一部で毛細血管壁の破綻が直後から観察され,4週後に一部線維化を後遺した。2.血管鋳型法による観察では,42℃加温群では,加温直後から24時間後まで表層の毛細血管の欠損が小範囲でみられたが,48時間後には欠損はみられなかった。43℃加温群では,直後から48時間後まで血管の欠損した部分が加温領域の広範囲に認められ,1週後にも血管網の形態変化がみられた。3.以上より,正常血管は42℃40分の加温では,一過性の血行障害が生じる可能性があるものの,48時間前後で回復し,非可逆的な血管障害は生じないが,43℃40分の加温では,相当の血行障害および一部で非可逆的な血管障害も生じることが示唆された。4.血管鋳型法を用いた本実験モデルは,-イパーサーミアによる血管障害を観察するうえで非常に有用であった0}, pages = {25--36}, title = {血管鋳型法を用いたハムスター正常舌のRFハイパーサーミアによる経時的変化に関する研究}, volume = {27}, year = {1997} }