{"created":"2021-03-01T06:30:54.626767+00:00","id":25441,"links":{},"metadata":{"_buckets":{"deposit":"1cd06a29-e2ff-47ac-8219-458045fa5d59"},"_deposit":{"id":"25441","owners":[],"pid":{"revision_id":0,"type":"depid","value":"25441"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00025441","sets":["432:1291:1292:1298","453:456"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Analysis of interaction effect between vascular endothelial growth factor and their receptors in human oral squamous cell carcinoma cell lines : mRNA expression levels for VEGF-A and VEGF-C"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"17","bibliographicPageStart":"11","bibliographicVolumeNumber":"40","bibliographic_titles":[{"bibliographic_title":"新潟歯学会雑誌"},{"bibliographic_title":"新潟歯学会雑誌","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ヒト口腔扁平上皮癌細胞株（HSC-2，HSC-3，HSC-4，OSC-19）においてVEGF-A mRNA 発現量が高い細胞株（HSC-2，OSC-19）ではVEGF-C mRNA 発現量も高いとされている。そこで本研究は，ヒト口腔扁平上皮癌細胞株における癌細胞が発現するVEGF-A，VEGF-C， およびVEGF 受容体（VEGFR（VEGFR-1，VEGFR-2，VEGFR-3））の相互作用を調べることを目的として行った。　Reverse transcription polymerase chain reaction（RT-PCR）法を用い，ヒト口腔扁平上皮癌細胞株（HSC-2，HSC-3，HSC-4，OSC-19）におけるVEGFR 発現の有無を確認した。さらに４種全てのヒト口腔扁平上皮癌細胞株に外部刺激としてヒトrecombinant VEGF-A およびVEGF-C 添加することによるVEGF-A，VEGF-C， およびVEGFR mRNA 発現量の増加をreal-time RT-PCR 法で定量した。　その結果VEGFR は４種全てのヒト口腔扁平上皮癌細胞株においてVEGFR-1 のみ発現しており，またヒトrecombinant VEGF-A およびVEGF-C 添加によるVEGF-A，VEGF-C，およびVEGFR mRNA の発現量に明らかな相互作用はみられなかった。　このことからヒト口腔扁平上皮癌細胞株においてはVEGF-A，VEGF-C，およびVEGFR mRNA 発現量に相互作用はないものと考えられた。　今後はヒトにおける口腔扁平上皮癌が発現するVEGF-A，VEGF-C，およびVEGFR の相互作用を明らかにするために，更なる研究が必要である。","subitem_description_type":"Abstract"},{"subitem_description":"Among in human oral squamous cell carcinoma (OSCC) cell lines HSC2, HSC-3, HSC-4, and OSC-19, HSC-2 and OSC-19 cell lines that were highly expressed VEGF-A mRNA showed high of VEGF-C mRNA expression. The purpose of the present study was to clarify that interaction of VEGF-A, VEGF-C, and VEGF receptors (VEGFR(VEGFR-1, VEGFR-2, and VEGFR-3)) in human OSCC cell lines.　We investigated the expression of VEGFR in human OSCC cell lines (HSC-2, HSC-3, HSC-4, and OSC-19) by reverse transcription polymerase chain reaction (RT-PCR). Furthermore, whether the expression levels of VEGF-A, VEGF-C, and VEGFR mRNA may be changed adding the human recombinant VEGF-A and VEGF-C, we examined the expression levels of VEGF-A, VEGF-C, and VEGFR mRNA by real-time RT-PCR in cultured human OSCC cell lines.　In each cell line expression of VEGFR-1 mRNA was detected, however, that of VEGFR-2 and VEGFR-3 mRNA were not detected. Expression levels of VEGF-A, VEGF-C, and VEGFR-1 mRNA when added to human recombinant VEGF-A and VEGF-C did not have statistically significant differences.　In OSCC cell lines, the expression levels of VEGF-A, VEGF-C, an VEGFR did not have any correlations in each other.　Further studies were required to clarify the correlation of VEGF-A, VEGF-C, and VEGFR in human OSCC.","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"149976","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Kanemaru, Shohei"}]},{"nameIdentifiers":[{"nameIdentifier":"149977","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Nakazato, Takayuki"}]},{"nameIdentifiers":[{"nameIdentifier":"149978","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Kitamura, Nobutaka"}]},{"nameIdentifiers":[{"nameIdentifier":"149979","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Shingaki, Susumu"}]},{"nameIdentifiers":[{"nameIdentifier":"149980","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Saito, Chikara"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟歯学会"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN0018300X","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03850153","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"金丸, 祥平"}],"nameIdentifiers":[{"nameIdentifier":"149971","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"中里, 隆之"}],"nameIdentifiers":[{"nameIdentifier":"149972","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"北村, 信隆"}],"nameIdentifiers":[{"nameIdentifier":"149973","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"新垣, 晋"}],"nameIdentifiers":[{"nameIdentifier":"149974","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"齊藤, 力"}],"nameIdentifiers":[{"nameIdentifier":"149975","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-19"}],"displaytype":"detail","filename":"NS_40(1)_11-17.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"NS_40(1)_11-17.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/25441/files/NS_40(1)_11-17.pdf"},"version_id":"c8a64dd2-9e95-4044-84ca-cfc0986a9155"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"口腔扁平上皮癌","subitem_subject_scheme":"Other"},{"subitem_subject":"VEGF-A","subitem_subject_scheme":"Other"},{"subitem_subject":"VEGF-C","subitem_subject_scheme":"Other"},{"subitem_subject":"VEGF 受容体","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ヒト口腔扁平上皮癌細胞株における血管内皮細胞増殖因子と血管内細胞増殖因子受容体の相互作用 : VEGF-A およびVEGF-C 刺激によるmRNA 発現レベルの動態","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ヒト口腔扁平上皮癌細胞株における血管内皮細胞増殖因子と血管内細胞増殖因子受容体の相互作用 : VEGF-A およびVEGF-C 刺激によるmRNA 発現レベルの動態"},{"subitem_title":"ヒト口腔扁平上皮癌細胞株における血管内皮細胞増殖因子と血管内細胞増殖因子受容体の相互作用 : VEGF-A およびVEGF-C 刺激によるmRNA 発現レベルの動態","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","1298"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-09-04"},"publish_date":"2015-09-04","publish_status":"0","recid":"25441","relation_version_is_last":true,"title":["ヒト口腔扁平上皮癌細胞株における血管内皮細胞増殖因子と血管内細胞増殖因子受容体の相互作用 : VEGF-A およびVEGF-C 刺激によるmRNA 発現レベルの動態"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:55:21.579124+00:00"}