{"created":"2021-03-01T06:06:14.432236+00:00","id":2525,"links":{},"metadata":{"_buckets":{"deposit":"5e4f59ef-9264-4ff1-9342-09e7c1dbeb35"},"_deposit":{"id":"2525","owners":[],"pid":{"revision_id":0,"type":"depid","value":"2525"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00002525","sets":["453:454","485:486:487"]},"author_link":["37614","37615","37616","37617","37618","37619","37620"],"control_number":"2525","item_1627363077551":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2003-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"194","bibliographicPageStart":"187","bibliographicVolumeNumber":"20","bibliographic_titles":[{"bibliographic_title":"Plant biotechnology","bibliographic_titleLang":"en"}]}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Effects of phosphate on the Ca2+ uptake and the sucrose-controlled secretion of α-amylase molecules in cultured rice cells were investigated. Phosphate markedly stimulated Ca2+ uptake into rice cells, particularly at the outer cell layer of the cell cluster. Phosphate also increased the synthesis and extracellular liberation of α-amylase II-4 in sucrose-supplemented cells. The distribution pattern of enzyme in rice cell clusters induced by phosphate was similar to that of Ca2+ uptake. Phosphate did not increase the level of mRNA of α-amylase II-4, indicating that phosphate stimulates the translation and posttranslational secretory processes of α-amylase II-4 in the presence of sucrose. Furthermore, phosphate enhanced both the Ca2+ uptake and α-amylase II-4 synthesis in the microsomes. These results strongly suggested that the ratio of phosphate to sugar is important for regulating the Ca2+ uptake, and that phosphate and sugar precisely coordinate the Ca2+-mediated synthesis and extracellular liberation of α-amylase II-4.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_5_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本植物細胞分子生物学会","subitem_publisher_language":"ja"}]},"item_5_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://doi.org/10.5511/plantbiotechnology.20.187","subitem_relation_type_select":"DOI"}}]},"item_5_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_5_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11250821","subitem_source_identifier_type":"NCID"}]},"item_5_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1342-4580","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Asatsuma, Satoru","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"37614","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nanjo, Yohei","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"37615","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kashem, Mohammad A.","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"37616","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Itoh, Kimiko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"37617","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hori, Hidetaka","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"37618","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ohshima, Masahiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"37619","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mitsui, Toshiaki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"37620","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-29"}],"displaytype":"detail","filename":"ZL_20(3)_187-194.pdf","filesize":[{"value":"1.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ZL_20(3)_187-194.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/2525/files/ZL_20(3)_187-194.pdf"},"version_id":"f1aab8a6-3a9f-43d7-a976-76f821cbcec5"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"α-amylase","subitem_subject_scheme":"Other"},{"subitem_subject":"Ca2+","subitem_subject_scheme":"Other"},{"subitem_subject":"cell culture","subitem_subject_scheme":"Other"},{"subitem_subject":"Oryza sativa L.","subitem_subject_scheme":"Other"},{"subitem_subject":"phosphates","subitem_subject_scheme":"Other"},{"subitem_subject":"sucrose","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Phosphate modulates Ca2+ uptake and α-amylase secretion in suspension-cultured cells of rice","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Phosphate modulates Ca2+ uptake and α-amylase secretion in suspension-cultured cells of rice","subitem_title_language":"en"}]},"item_type_id":"5","owner":"1","path":["454","487"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2012-04-04"},"publish_date":"2012-04-04","publish_status":"0","recid":"2525","relation_version_is_last":true,"title":["Phosphate modulates Ca2+ uptake and α-amylase secretion in suspension-cultured cells of rice"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-15T03:35:44.018400+00:00"}