@article{oai:niigata-u.repo.nii.ac.jp:00002525,
 author = {Asatsuma, Satoru and Nanjo, Yohei and Kashem, Mohammad A. and Itoh, Kimiko and Hori, Hidetaka and Ohshima, Masahiro and Mitsui, Toshiaki},
 issue = {3},
 journal = {Plant biotechnology},
 month = {Jun},
 note = {Effects of phosphate on the Ca2+ uptake and the sucrose-controlled secretion of α-amylase molecules in cultured rice cells were investigated. Phosphate markedly stimulated Ca2+ uptake into rice cells, particularly at the outer cell layer of the cell cluster. Phosphate also increased the synthesis and extracellular liberation of α-amylase II-4 in sucrose-supplemented cells. The distribution pattern of enzyme in rice cell clusters induced by phosphate was similar to that of Ca2+ uptake. Phosphate did not increase the level of mRNA of α-amylase II-4, indicating that phosphate stimulates the translation and posttranslational secretory processes of α-amylase II-4 in the presence of sucrose. Furthermore, phosphate enhanced both the Ca2+ uptake and α-amylase II-4 synthesis in the microsomes. These results strongly suggested that the ratio of phosphate to sugar is important for regulating the Ca2+ uptake, and that phosphate and sugar precisely coordinate the Ca2+-mediated synthesis and extracellular liberation of α-amylase II-4.},
 pages = {187--194},
 title = {Phosphate modulates Ca2+ uptake and α-amylase secretion in suspension-cultured cells of rice},
 volume = {20},
 year = {2003}
}